We narrowed to 114 results for: CHL
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TypeBlog Post... Susanna Bachle...
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Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post... Susanna Bachle... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...standard method for purification of rAdV uses cesium chloride (CsCl) density gradients combined with ultracentrifugation... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...Roth's and Takafumi Minamimoto's lab called deschloroclozapine (DCZ) shows promise as a CNO alternative ... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post... apramycin, blasticidin, bleocin (zeocin), chloramphenicol, erythromicin, gentamicin, hygromycin, kanamycin... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post...JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev ... -
Hot Plasmids: Winter 2025
TypeBlog Post... gene to confer resistance to the herbicide chlorsulfuron (Homo line 2) or wild type (WT), two weeks after... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post... Susanna Bachle... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post...e.g. p15A, ColE1) can be increased by adding chloramphenicol to the culture medium. Improve the culture ... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post..., Chug, H., Görlich, D., Lee, C., Pleiner, T., Schliep, J.E., Stark, H., Trakhanov, S., & Urlaub, H. (... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post...Additional Resources Zhang lab Cpf1 blog post on Benchling ... -
CRISPR-mediated Plant Base Editors
TypeBlog Post...base editing. They first created a defective chloramphenicol resistance gene (CamR) by introducing a point... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...Kaze, Christina Kellogg, Heather Martinez, Pat Schloss, Tara Smith, Marc Sze, Victoria McGovern, and Julie... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...cannot be studied in hairy roots, as functional chloroplasts and leaf specific cell types are missing in ... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...Cepko, C., Guerin, K. I., Rego, M. A., Ersing, I., Bachle, S. M., Kamens, J., & Fan, M. (2019). Adeno-Associated... -
Transferable Skills Guide: External Collaborations
TypeBlog Post... Susanna Bachle... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...the inner membrane space of mitochondria and chloroplasts, the extracellular milieu, and gram negative... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog Post...Snell KD, Seo JS, Chua N-H, Strano MS (2019) Chloroplast-selective gene delivery and expression in planta... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog Post...hsdR17(rK- mK+) XL10 Gold Tetracycline and Chloramphenicol High competency cloning and propagation of ... -
Viral Vectors 101: Optogenetic Tools
TypeBlog Post...light-activated cation channel, from the archaebacteria Chlamydomonas reinhardtii. When exposed to blue light, this... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...27966527. PubMed Central PMCID: PMC5171807. 6. Machleidt, T., et al. (2015) NanoBRET-A novel BRET platform... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post... guide you in gRNA design. We have been using Benchling, but there are many other gRNA design tools available... -
Brain Initiative Collection
TypeCollection...AAV9 pAAV_hSynapsin_psychLight2 To generate Adeno-Associated viruses for expression of psychLight2 under... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...pyogenes Chloramphenicol Marraffini pCas9 42876 Bacteria BsaI yes, cut S. pyogenes Chloramphenicol Marraffini... -
CRISPR Guide
TypeCollection...alternatives Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J., Belanto, J. J., Verdine...Gootenberg, J. S., Abudayyeh, O. O., Lee, J. W., Essletzbichler, P., Dy, A. J., Joung, J., Verdine, V., Donghia..., O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., Van Der Oost, ... -
Typing CRISPR Systems
TypeBlog Post...References Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J., Belanto, J. J., Verdine... -
Selecting Your Plasmid Purification Kit
TypeBlog Post...kits are quick, easy, and don’t involve phenol-chloroform. The default option is to use the kit that ... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post... of two plasmids: pCas9: carries Cas9 and chloramphenicol resistance pCRISPR: carries a spacer targeting... -
Optogenetics Plasmid Collection
TypeCollection... widely used Channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii . ChR2 is a membrane channel that... -
Brzezinski Lab CRISPR Collection
TypeCollection.... Usage Guide sequences can be designed using Benchling or using similar design tools and ordered as standard...