We narrowed to 154 results for: 11
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TypeCollection...Rinehart J, Huttlin EL, Gygi SP. Nat Methods 2022 Nov;19(11):1371-1375. doi: 10.1038/s41592-022-01638-5. PubMed...
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CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post...Mycobacterial species, Sarah Fortune’s lab screened 11 other Cas9 orthologs to identify a more efficient... Phage-Delivered CRISPR-Cas3 Antimicrobials. mBio 11: . https://doi.org/10.1128/mbio.00019-20 Singh AK... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...expression of human IL1RN, the researchers saw an 11-fold increase in mRNA levels after 2 hr and a 400...control of endogenous gene activation. Nat Chem Biol 11:198–200 . https://doi.org/10.1038/nchembio.1753 Richter... -
Delivery Methods for Generating iPSCs
TypeBlog Post...generating iPSCs by plasmid transfection where 2 of 11 clones tested had plasmid integration. Another challenge...Nonstructural C Protein and Endogenous MicroRNAs." Plos One 11, no. 10 (2016). PubMed PMID: 27764162. PubMed Central... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...12005-15. Hernandez et al., Autophagy 2013 Nov 1;9(11):1852-61. A quicker method for protein inactivation...variants. Klapoetke et al., Nat Methods 2014 Mar 11;(3):338-46. For more information and descriptions....3179. Martell et al., Nat Biotechnol 2012 Nov;30(11):1143-8. A new tool for CRISPR gRNA validation... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...non-human primate brain. Nature Nanotechnology, 1–11. https://doi.org/10.1038/s41565-023-01419-x Deverman...cellular receptor for AAV-PHP.B capsids. PloS One, 14(11), e0225206. https://doi.org/10.1371/journal.pone.0225206...prioritization for cell type-specific enhancer design. eLife,11, e69571. https://doi.org/10.7554/eLife.69571 Liguore...Primates Compared with Mice. Molecular Therapy, 27(11), 2018–2037. https://doi.org/10.1016/j.ymthe.2019.07.017... -
Summer SciComm Series: Cognitive Load
TypeBlog Post...-page genre fiction novella as it does to read an 11-page Cell paper - and sometimes, it might actually... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...other labs discovered that the in vitro functions of 11 different Arg-mutant enzymes could be rescued by ... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...subtypes, based on their Gα subunit: Gαs, Gαi/o, Gαq/11, and Gα12/13. Gβ and Gγ are closely associated and... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...PMID: 26280411. PubMed Central PMCID: PMC4785103. 11. Wong, Alan SL, et al. "Multiplexed barcoded CRISPR-Cas9... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog Post...EMX1 locus in human embryonic kidney (HEK) cells, 11 of these initial mutants retained the on-target activity... -
Plasmids 101: Golden Gate Cloning
TypeBlog Post...Engler C, Kandzia R, Marillonnet S. PLoS One 2008;3(11):e3647. doi: 10.1371/journal.pone.0003647. Epub 2008... -
Protocol - How to Run an Agarose Gel
TypeProtocol... you want to load 1.0 μg in 10 μL, make 1.1 μg in 11 μL. Reference Page Top Index... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...of the chimeric and fully albino mice ranging from 11% to 100%, a level similar to other approaches. Nanoblades... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...Dujon, B. Yeast evolutionary genomics. Nat Rev Genet 11, 512-524. (2010). PubMed PMID: 20559329. 8. Reeves... -
Virus Protocol - Generating Stable Cell Lines
TypeProtocol...Sanjana NE, Shalem O, Zhang F. Nature Methods. 2014 Aug;11(8):783-4 (Link opens in a new window) . Last reviewed... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...genome editing with engineered nucleases. Nat Methods 11:1051–1057 . https://doi.org/10.1038/nmeth.3075 Lukashev... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog Post...in science and scientists (as discussed in Chapter 11)? Even if some of the problems are addressed in a... -
Plasmids 101: Degron Tags
TypeBlog Post...via a self-excising degron. Nat Chem Biol. 2015 Sep;11(9):713-20. doi: 10.1038/nchembio Nabet B, Roberts... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post... CRISPR-Cas9 with the PITCh systems.” Nat Protoc. 11(1): 118-33. PMID: 26678082. Find the plasmids from... -
Rett Syndrome
TypeCollection...correlate with neuronal maturation. Hum Mol Genet . 11, 115–124. PMID: 11809720 Tarquinio et al. 2015. Age... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post... windows as 01/15/20 -- 03/15/20 for China and 03/11/20 -- 05/19/20 (the last date in our dataset) for... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...organization deciphering. Molecular Cytogenetics, 11. https://doi.org/10.1186/s13039-018-0368-2 Liu X... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog Post... scientific disciplines. Scientific Data, 8(1), 1–11. https://doi.org/10.1038/s41597-021-00981-0 Wright... -
Hot Plasmids: Winter 2025
TypeBlog Post...members of all four G protein families: Gs, Gi/o, Gq/11, and G12/13. Find the ONE-GO Biosensors plasmids ... -
Degrading DNA with Cascade-Cas3
TypeBlog Post...CRISPR–Cas systems. Nature Reviews Microbiology, 13(11), 722–736. https://doi.org/10.1038/nrmicro3569 Additional... -
Magnetic Control of Proteins: More than a Dream
TypeBlog Post...Fields Using a Radical Pair Reaction. Nano Letters, 11(12), 5367–5372. https://doi.org/10.1021/nl202950h... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog Post...Data-optimized Assembly Design. ACS Synthetic Biology, 11(6), 2036–2042. https://doi.org/10.1021/acssynbio.1c00525... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post...Analytical Chemistry 27(2):108-117 · February 2008. 11. Svensen, Nina and Jaffrey, Samie R. “Fluorescent... -
MXS Chaining
TypeBlog Post...with high throughput capability. PloS one. 2008;3(11):e3647 doi: 10.1371/journal.pone.0003647. Pubmed ... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog Post... T, Fujii H. Biochem Biophys Res Commun. 2013 Aug 11. pii: S0006-291X(13)01329-6. doi: 10.1016/j.bbrc.2013.08.013... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post...;9(6):658-66. doi: 10.1038/ni.1611. Epub 2008 May 11.) PubMed. Read more Plasmids 101 posts or browse... -
Transferable Skills Guide: Creativity
TypeBlog Post...Retrieved January 14, 2022, from https://hbr.org/2019/11/5-mental-mistakes-that-kill-your-productivity Finkelstein... -
Viral Vectors 101: Pseudotyping
TypeBlog Post... 100:823–832 . https://doi.org/10.1182/blood-2001-11-0042 Takeuchi Y, Liong SH, Bieniasz PD, Jäger U, ... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog Post...biosynthesis, we constructed a strain library with 1-11 copies of integrated genes. We obtained a stable ... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog Post...accuracy of western blots. Expert Review of Proteomics, 11(5), 549–560. https://doi.org/10.1586/14789450.2014.939635... -
An Introduction to Adenovirus
TypeBlog Post...Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https://doi.org/10.1371/journal.ppat... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...Phosphotyrosyl Linkage.” Molecular and Cellular Biology 5(11): 3274–79. PubMed PMID: 2427927. PubMed Central PMCID... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...CRISPR-Cas9 system. Ran FA, et al. Nature Protocols Nov;8(11):2281-308; (2013). PubMed. A Mouse Geneticist's... -
Site Directed Mutagenesis by PCR
TypeBlog Post...Experimental guidelines Primer Design As a rule of thumb, 11 bp of complementary sequence on either side of the...