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We narrowed to 185 results for: INV-E

Showing: 81 - 120 of 185 results

  1. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ... phosphorylated amino acid to the genetic code of E. coli (Park et al., Science 2011). The Rinehart lab... methodology in two differents types of bacteria (E. coli and Salmonella typhimurium) by integrating DNA...expression and purification of Cas9 protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor...complex constructs constrained only by the ability of E. coli to maintain the final plasmid after transformation... complex DNA constructs, particular for those involving recombination elements such as FRT or Lox sites...of sequences in bacteria are time-consuming and involve many steps. The Drew Endy and Keith Shearwin labs...molecules, allowing labs to precisely and non-invasively control neuronal signaling. For more information...

  2. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...library of E. coli tRNA adenosine deaminases (ecTadA), fused it with dCas9, and transformed it into E. coli... (Komor et al., 2016 and Nishida et al., 2016) invented CRISPR-base editors by tethering cytosine deaminase...

  3. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...University of Pennsylvania.     References: 1. Lee HJ, Kim E, Kim JS. Targeted chromosomal deletions in human cells...efficiency of the method. One observation was an inverse relationship between deletion size and frequency...outcome to deletions following paired DSBs is inversions [3]. These rearranged segments may also be informative...nondeleted allele is likely to bear an indel or inversion mutation [3]. In the CRISPR era, the genomic landscape...Bauer is a pediatric hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical...

  4. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...recombination persists even in recombinase deficient E. coli and under conditions used to minimize recombination...Bourges D, Ersing I, Haery L, Harten DeMaio K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L...reading frame is inverted in relation to the promoter, hence the name, double-floxed inverted open-reading...transgene is no longer able to flip back into the inverse orientation (See Plasmids 101: FLEx Vectors). While...opposite orientation (direction of arrows) the inverted transgene is reverted into the sense orientation...

  5. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...10.1534/genetics.111.131433 Doudna JA, Charpentier E (2014) The new frontier of genome engineering with...CS, Ralston EJ, Lee AH, Amora R, Miller JC, Leung E, Meng X, Zhang L, Rebar EJ, Gregory PD, Urnov FD, ...testing and modifications. The premise of the novel involves Dr. Moreau who creates human-animal hybrids. This... the discovery of meganucleases. The storyline involves the resurrection of dinosaurs by extracting the...Tom Hanks as the protagonist (Langdon). The plot involves Langdon chasing an anti-villain geneticist named...which goes horribly wrong. The plot of the movie involves The Rock and his super giant albino gorilla fighting...

  6. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...greatly simplified the process of generating multiple E. coli gene expression plasmids with the Cross-disciplinary...gate cloning that results in the creation of many E. coli constructs with greatly varied levels of expression...well as a dominant-negative mutator allele of the E. coli MMR protein MutL, all under the control of the... MutL is highly conserved in distant relatives of E. coli so it can be used in a broad range of strains...domains, but these domains often do not express well in E. coli without their partner regulatory domains. The...library and used this library to screen for genes involved in West Nile Virus (WNV) induced cell death. The...for their gene of interest. Although the parts involved in altering gene expression are relatively well...

  7. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ... Sommella A, Iodice C, Cesi G, Simone S, Marrocco E, Rossi S, Giunti M, Palfi A, Farrar GJ, Polishchuk...doi.org/10.1002/emmm.201302948 Trapani I, Toriello E, de Simone S, Colella P, Iodice C, Polishchuk EV, ...results in effective gene delivery in mice. J Clin Invest 118:1955–1964 . https://doi.org/10.1172/jci34316...

  8. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ....org/10.1126/science.1231143 Doench JG, Hartenian E, Graham DB, Tothova Z, Hegde M, Smith I, Sullender...doi.org/10.1038/s41598-018-33408-9 Mizuno N, Mizutani E, Sato H, Kasai M, Ogawa A, Suchy F, Yamaguchi T, Nakauchi...easy. In the 1980s, gene targeting technology was invented to introduce specific changes into the mouse genome...

  9. Reflections and Looking Ahead

    Type
    Blog Post
    ..., A. I., Saper, C. B., Errington, T. M., Turn, R. E., Bandrowski, A., Trimmer, J. S., Rego, M., Freedman... plasmids — as DNA or bacterial stabs — ‌and reinventing our search experience so you can find exactly...

  11. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...grow in the coming decades.  References 1. Kowarz E, et al. Optimized Sleeping Beauty transposons rapidly...) The transposon consists of a mirrored set of inverted repeats (green) flanking the gene of interest ... enzyme is expressed (red stars) and binds the inverted repeats (green); and an endonuclease reaction ...

  16. What the HEK?

    Type
    Blog Post
    ...2002) (8):869-71. DOI: 10.1096/fj.01-0995fje Tan, E., et al., HEK293 Cell Line as a Platform to Produce...interests include receptor biology, ion channels, or involve human protein preparations with PTMs. Why the HEK...

  18. How Addgene Creates Content

    Type
    Blog Post
    ...website, social media, videos, newsletters, guides, and e-books – comes into play. Our goal is to provide informative...post, we invite you behind the scenes in our content writing process, and to even get involved yourself...standards. It’s always a work in progress! Get involved A lot of our content already comes from you and...depositors and customers are asking. So how can you get involved? Keep asking your burning questions and tell us...

  19. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide. It was designed based on MYC-ChIP-seq...lab targets human genes known or suspected to be involved in DNA damage response and DNA repair.  A new ...

  20. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...10.1039/C8CS00710A Sahasrabudhe, A., Rupprecht, L. E., Orguc, S., Khudiyev, T., Tanaka, T., Sands, J., ...animals being monitored were physically hooked up to invasive, cumbersome systems during experiments – neither...

  21. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...Standards Synthetic Photobiology: Optogentics for E. coli Browse All Synthetic Biology Blog Posts Resources... into modules that students and teachers can investigate together. Teachers decide which BioBuilder lessons...of teachers around the country. How you can get involved with BioBuilder Details for joining BioBuilder...

  22. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...gene function. RNAi is commonly achieved by feeding E. coli expressing dsRNA to C. elegans. These dsRNAs...vector, L4440, places a target gene between two inverted T7 promoters. Transcription from each promoter...Src homology 3 (SH3) domain family, known to be involved in cytoskeletal control, cell growth, and endocytosis...carry out any biochemical and biophysical study involving protein stability or binding experiments. Potential...

  23. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ..., Carr, S., Edwards, A., Ellenberg, J., Lundberg, E., Rimm, D. L., Rodriguez, H., Hiltke, T., Snyder, ...M. S., Smallwood, D. T., Weston, C. A., Castells, E., Bowman, V. W., McCarthy, J., Amrani, Y., Duffy, ...not in the iso-genic knockout control. This can involve cell surface labeling and/or intracellular labeling...overexpress the target of interest. This approach often involves the transient transfection of expression plasmids...

  24. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...References 1. Bressan RB, Dewari PS, Kalantzaki M, Gangoso E, Matjusaitis M, Garcia-Diaz C, Blin C, Grant V, Bulstrode..., Southgate B, Mccarten K, Monogarov G, O'Duibhir E, Quinn N, Tyrer A, Leitner MC, Plumb C, Kalantzaki...purchased directly from commercial suppliers without investing time and labour on reagent preparation in the ...

  25. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...can easily sort them by other parameters, such as E-value for protein sequence searches. Additionally,... an evolving database, and all researchers are invited to contribute to it! The CasPEDIA Consortium will...update entries as research progresses, but you are invited to reach out to suggest new entries or even new...

  26. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...2016). PubMed PMID: 26814968. McCarty, D. M., Paul E. Monahan, and Richard J. Samulski. "Self-complementary...gutted”, meaning that all viral genes except ITRs (inverted terminal repeats – the sequences that flank the...a Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal circuits of the retina using viral ...

  27. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ... Hendrickson EA, Giovannangeli C, Jasin M, Brunet E (2014) Chromosomal Translocations in Human Cells Are...the initial cut, the next steps in the process involve repairing chromosomal DSBs. It is important to ...

  28. Summer SciComm: Preprints

    Type
    Blog Post
    .../2021.09.07.459259 Sarabipour S, Debat HJ, Emmott E, Burgess SJ, Schwessinger B, Hensel Z (2019) On the...details that you couldn’t fit on your poster, or invite attendees to read and comment online! Want the ...

  29. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...allow students to propagate the plasmids in either E. coli (ori and ampR) or S. cerevisiae (2µm ori and...answer questions about the evolution of the genes involved in methionine synthesis (5). The budding yeast...and instructor resources is available at our Investigations in Molecular Cell Biology Website. Many thanks...

  30. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...genome editing tool.   References Mangeot, Philippe E., et al. "Genome editing in primary cells and in vivo...approaches.  Nanoblades can also be used to non-invasively gene edit the liver of adult mice. When mice ...generating transgenic mice strains and for non-invasive in vivo gene editing. The bottom line on Nanoblades...

  31. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...expansion.  References and Resources References Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A...in cell-free systems. These pathways typically involve multiple steps in which a precursor molecule is...

  32. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...Let us know about it in the comments or shoot us an e-mail at [email protected] and maybe we can write a ...blog post: Synthetic Photobiology: Optogenetics for E. coli. Schmidl, et al. ACS Synth Biol. 2014 Nov...aggregation propensity in four expression systems: E. coli, wheat germ, HeLa, and Leishmania (Gagoski, ...origin of replication increases plasmid copy number in E. coli. The authors used pXPG to study the human GM-CSF...nucleases, recombinases) which irreversibly excise or invert a specific chunk of DNA. However, a limitation ...genome-engineering. One particularly attractive application involves using dCas9-activator fusion proteins to induce...a “neuron-like” phenotype when targeting genes involved in neurogenesis, which had proven challenging ...

  33. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...Saloman, J. L., Scheff, N. N., Snyder, L. M., Ross, S. E., Davis, B. M., & Gold, M. S. (2016). Gi-DREADD Expression...a popular tool in the field as it provides a noninvasive method for manipulating cell activity, especially...provided by chemogenetics are instead that it is noninvasive and has longer-lasting effects. Building your...specific circuits. Methods for targeting expression involve tissue specific promoters, AAV serotypes, and the...

  34. Of Myc and Men

    Type
    Blog Post
    ...(87)90086-9  Schüchner S, Behm C, Mudrak I, Ogris E (2020) The Myc tag monoclonal antibody 9E10 displays...Pelham’s first published peptide tagging experiment involved a peptide from the neuropeptide substance P. This...suitable pair for protein tagging. In two studies investigating mechanisms of endoplasmic reticulum localization...versions of Myc1-9E10 mentioned above basically involve taking the Myc1-9E10 variable regions and sticking...

  35. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...currently shared amongst the zebrafish community. Don E, et al. Zebrafish. 2017. PubMed PMID: 27631880 Kwan...Bacterial Expression Construct SpCas9 Inhibition (E. coli) Mammalian Expression Construct SpCas9 Inhibition...improve the study of specific neuronal cell types involved in neurodegenerative diseases, the Cole Lab selected...to premature aging and cancer. The Denchi lab investigated an aspect of telomere length regulation called...

  36. Hot Plasmids February 2024

    Type
    Blog Post
    ...doi.org/10.21203/rs.3.rs-2941917/v1.  Ivorra-Molla, E., et al. (2023). A monomeric StayGold fluorescent ...adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene delivery in non-human primate (NHP...This new capsid will be a valuable tool for investigating NHP brains. Figure 3: Overview of library...

  38. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...Pubmed Central PMCID: PMC1222268. 7. Aronson, Deborah E., Lindsey M. Costantini, and Erik L. Snapp. "Superfolder...the cytoplasm. That is, unfortunately, some investigators realize too late that they have missed the impact...behaving properly, FPs enable researchers to investigate the localization and dynamics of fluorescent ...environment where it will localize [4]. Most investigators are surprised to learn that FPs are often poorly...overestimation of fusion protein activity. We encourage investigators to characterize FP-fusion proteins functionally...

  39. CRISPR Guide

    Type
    Collection
    ...36593413 Vo, P. L. H., Ronda, C., Klompe, S. E., Chen, E. E., Acree, C., Wang, H. H., & Sternberg, S. H... R., Hanna, R. E., Hegde, M., Donovan, K. F., Strand, C., Sullender, M. E., Vaimberg, E. W., Goodale, ...edits. PASSIGE ( P rime- E diting- A ssisted S ite- S pecific I ntegrase G ene E diting), developed by the.... PMID: 26735016 Lee, J. K., Jeong, E., Lee, J., Jung, M., Shin, E., Kim, Y., Lee, K., Jung, I., Kim, ..., K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013). RNA-Guided Human...Siouve, E., Becavin, C., Depardieu, F., & Bikard, D. (2018). Genome-wide CRISPR-dCas9 screens in E. coli...e1007749. PMID: 30575746 Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S.,...

  40. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ... CBE Life Sciences Education. 21(4):ar74. Duboue, E., Kowalko, J., Keene, A. et al. 2022. Course-based...ownership of their work and become more actively involved in the learning process. As trainees become more... and effort and/or perceived professional risk involved in setting up a CURE, especially if it’s a new...
Showing: 81 - 120 of 185 results