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We narrowed to 253 results for: Mac

Showing: 81 - 120 of 253 results

  1. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ... which is then read by a detector panel in the machine. Fluorophores are light sensitive, so antibodies...idea to make sure that laser is available in your machine before you make your purchase. Picking a single...

  2. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...recombineering, bacteria express phage recombination machinery, which facilitates easy genetic modification by...electrocompetent cells in which the phage recombination machinery is induced using L-arabinose. These cells can ...

  3. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...instead use a preexisting, conserved cellular machinery to process gRNAs. Hijacking tRNA processing to...plants. Yang was intrigued by the tRNA processing machinery present in almost every living organism. Precursor...

  4. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...unless autophagy is induced. Then non-selective macroautophagy of the cytosol leads to accumulation of Pho8Δ60.... E. (2015). Rapid parallel measurements of macroautophagy and mitophagy in mammalian cells using a single...

  10. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ... in order to turn Twitter into a paper-finding-machine. There are four general types of accounts you can...Scholar analyzes publications and figures using machine learning to extract important features such as ...

  11. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...inherited the genomic edit but not the editing machinery. Together, these advances have the potential to.... Example applications include large-scale pharmacological profiling or interrogation of context-dependent...

  12. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...partially because they contain some of the necessary machinery to make these viruses. The cells are also easy...they require cloning specific AAV or lentiviral machinery into a baculovirus plasmid. Production of baculovirus...

  13. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...to run larger panels: five laser spectral flow machines have 64 detectors, allowing for the use of up ...be distinguished using spectral flow since the machine uses the full spectral signature, not just a narrow...

  14. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...range of proteins including antibodies and pharmaceuticals, specifically interleukins. Yeast expression...components of the transcription and translation machinery. These include ribosomes, RNA polymerase, tRNAs...

  21. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...RNA that bind to a cell’s DNA, telling the Cas machinery where to cut.      Fig. 2: Heatmap indicating...): 250-252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter the weaponization of genetics research...

  24. CRISPR Antimicrobials

    Type
    Blog Post
    ...bacteria have poor non-homologous end joining machinery, so a CRISPR-induced double-stranded break (DSB...into the bacterial genome, and it carries CRISPR machinery targeting 1) the given resistance gene and 2) ...

  26. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...plasmids rely on their host cell's replication machinery in order to replicate—but not always to the same..., as they only require the host's replication machinery for elongation and termination. These types of...

  27. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ... plasmids to utilize diverse host replication machinery. For instance, the ori typically contains iterons...However, if plasmids have their own replication machinery such as Rep or other initiator proteins, they ...

  28. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...these approaches, such as delivery of the editing machinery to the brain, minimizing off-target editing, and...https://doi.org/10.3389/fgene.2019.00625 McGraw CM, Samaco RC, Zoghbi HY (2011) Adult Neural Function Requires...

  30. Evolution of Lab Techniques

    Type
    Blog Post
    ...generally considered first generation DNA sequencing machines (9). Today, similar technologies, sometimes referred...older technologies nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing...

  31. A Control for All Seasons

    Type
    Blog Post
    ...Submission to the British Journal of Pharmacology.” British Journal of Pharmacology, vol. 175, no. 3, Wiley, 19...

  33. Typing CRISPR Systems

    Type
    Blog Post
    ...involved in plasmid competition or can hijack machinery from other CRISPR systems to carry out various..., L., Kocalar, S., Oshiro, R., Makarova, K. S., Macrae, R. K., Koonin, E. V., & Zhang, F. (2023). Uncovering...

  34. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ... produces modified dendritic cells (DCs) and macrophages (M1 and M2) that help create welcoming environments...over-express CD47 to escape recognition by circulating macrophages. Tumor cells also downregulate the expression...

  36. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...observations: Rapamycin is a membrane-permeable pharmacological agent that can rapidly induction heterodimerization...the Roth lab to be activated by otherwise pharmacologically inert drug-like small molecules, allowing ...

  37. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... industry, the bioethanol industry, and the pharmaceutical industry, depend on the yeast, Saccharomyces..., including fermented beer and wine, fuels, pharmaceutical ingredients, and recombinant proteins. In order...

  38. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...prime editing systems through manipulating MMR machinery. On the fifth day of CRISPR, Addgene gave to me...

  39. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...that will keep the lab running like a well-oiled machine. Lab tip #1: Glove trick Putting on lab gloves ...

  40. Deck the Lab 2022!

    Type
    Blog Post
    ...everything from Falcon tube trees to giant paper mache Krampus performing routine lab maintenance tasks...
Showing: 81 - 120 of 253 results