We narrowed to 1,054 results for: STI

...PMID: 25398340 Walton, R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained...Publications CRISPR Overview Bacteria have an interesting adaptive immune response that detects and eliminates...DNA with no homology elsewhere in the genome. Realistically, most gRNA targeting sequences will have additional..., a large portion of the Cas9-induced DSBs will still be repaired via NHEJ. The resulting population of...integrase landing site ( attB ) and the other to stimulate DNA repair. This can result in more efficient ...activity. Knockout libraries often target 5′ constitutively expressed exons, while activation and repression...repression, many CRISPR epigenetic tools require constitutive expression of the dCas9 fusion and/or expression...

...syndrome is currently based on specific clinical diagnostic criteria, defined as the presence of relatively...gait. Greater than 95% of individuals who meet diagnostic criteria have disease-causing mutations in the...switched on regardless of age or symptom severity, suggesting that restoring MECP2 expression in humans could...that can be differentiated into neurons Coriell Institute: Patient-specific lines (Link opens in a new window... database of clinical features and genetic characteristics of Rett syndrome (Link opens in a new window...Repositories (Link opens in a new window) Coriell Institute - cell line repository, including patient-derived...18337588 Neul et al. 2010. Rett syndrome: revised diagnostic criteria and nomenclature. Ann Neurol . 68, 944...

...but they still have potential targetable sites approximately every 35bp. Researchers are still determining...dimer, cutting in the spacer region between two distinct ZF target sites. (B) A TAL effector nuclease (...available as a kit from Addgene , custom arrays consisting of 12-31 repeats can be assembled and inserted...Toolbox", from Dr. Feng Zhang’s group at the Broad Institute, will be available from Addgene before the end...to mutate a specific gene or your anxiously investigating gene therapy options for a rare disease in humans...constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia...

...GCE bulletin board (GCEbb) listserve and posting your questions there. This peer-to-peer GCE community ...provided in the media can be incorporated using the existing protein translation machinery. A synthetase plasmid...a protein so that it is responsive to specific stimuli, such as light. On a broader scale, the expanded...made when the ncAA is included. For any and all questions you have while designing, implementing, or troubleshooting...127411 pEVOL-pylT-N346A/C348A PylRS M. mazei mono-substituted phenylalanine derivatives and tyrosinyl ethers...pMAH-POLY-eRF1(E55D) POLY-eRF1(E55D) E. coli para-substituted phenylalanine analogs Mammalian TAG Huiwang Ai...ylamino)-2-amino-propanoic acid (Anap) Mammalian TAG Justin Taraska 242002 pSEVA621-AzFRS AzFRS M. jannaschii...

...pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive or inducible expression of cDNAs...Garry Nolan 12257 pWPXL 2nd EF-1alpha-driven constitutive transgene expression. Contains intron that gives...Didier Trono 12254 pWPI 2nd EF-1alpha driven constitutive transgene expression and EGFP coexpression. ...shRNA or cDNA expression, gateway plasmid, and constitutive hygromycin selection. See Shin et al. (2006)...Fraser 15948 pLOVE 3rd Tet inducible gateway destination plasmid for cDNA expression. Miguel Ramalho-Santos...Content last reviewed: 23 October 2025 Do you have suggestions for other plasmids that should be added to this...

...feedback and suggestions for new antibodies to add to the collection using our Antibody Suggestion Form . Browse...recommended applications based on our in-house testing and data provided by trusted outside labs – with...targeting proteins for neuroscience research. Institute for Protein Innovation Collection : Antibodies...Recommended Applications PI Return to Top Do you have suggestions for other antibodies that should be added to ... represent the official views of the National Institutes of Health. Need Some Help? Browse some of our...

...with the delivery of stochastic combinations of cell-filling antigenically distinct protein tags (or "bits...plasmids expressing the barcode proteins under the constitutive CAG promoter (Addgene #242764–242781), but E11...individual synapses. Figure 2: PRISM workflow. A) Stochastic infection of pooled AAVs delivered in vivo generates...window) , in collaboration with The Francis Crick Institute (Link opens in a new window) , MIT (Link opens...opens in a new window) , and The Max Planck Institute for Biological Intelligence (Link opens in a new window...

...such as titering, purity assessment, sterility testing, and viral genome sequencing.... process: Titering Purity assessment Sterility testing Viral genome sequencing (VGS) Find detailed pricing...Additional steps, including request approval, your institution’s approval of the MTA, and shipping, mean that...order. MTA Approval 1+ business days, varies by requesting organization Viral vector preps remain subject... typically takes 1–4 days, depending on your institution. AAV Production 6–8 weeks Quality is our priority...Addgene does not perform functional or in vivo testing. We recommend that you consult the original paper...

...212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription Debrya Groskreutz...hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating regions controlling transcription Pete Stecha...212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating regions controlling transcription Pete Stecha...Expression Constructs Luciferase driven by a strong constitutive promoter can be used to monitor transfection...NF-kb, TGF-b, c-Myc, p53, and MAPK/JNK plus a constitutive control. Koen Venken 133315 pMKV060 Firefly ...proteome stress. pHLuc : An extracellular pH sensor consisting of a pH-sensitive GFP (superecliptic pHluorin...items are updated more frequently. Do you have suggestions for other plasmids that should be added to this...

...activation plasmids and screening library CRISPR/Cas9 Synergistic Activation Mediator (SAM) is an engineered protein...the Zhang Lab established Cre-dependent and constitutively expressing Cas9 knockin mice (Platt et al ....sgRNA to a Cre-dependent mouse or sgRNA to a constitutively Cas9-expressing mouse. Described here are AAV...from Jackson Labs: Cre-dependent Cas9 mouse Constitutively active Cas9 mouse Available plasmids are described...plasmid can be digested using SapI, which will reveal sticky ends to enable the rapid ligation of annealed and...

...site) 165508 pOpen-HindIIIR HindIII 165526 pOpen-PstIR PstI 165528 pOpen-SfiIR (cDNA) SfiI (used to create...pOpen-Eco31IB Eco31IB DNA methyltransferase 165536 pOpen-PstIM M.PstI DNA methyltransferase 165538 pOpen-SfiIM M.SfiI...basic and applied fields as diverse as genomics, diagnostics, biodiversity, synthetic biology, DNA origami...pOpen-BovDNTT Bovine DNTT 165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top Additional Resources...

...years from symptom onset. Disease mechanisms are still poorly understood, but several genes and mapped ...other large scale studies. Researchers are now investigating the role that these additional genes may play...Zhuo et al. Nat Methods. 2023) Browse the Allen Institute's Enhancer Collection to target different cell ...and other difficult-to-target antigens in the Institute for Protein Innovation Antibody Collection . Explore...endogenous gene tagging in human cells from the Allen Institute for Cell Science Collection . Express EGFP-tagged...accelerate discovery and advance development of diagnostics and treatments for Alzheimer’s disease and related...

... distributed by Addgene were developed by the Institute for Protein Innovation (IPI). For these antibodies...more about these antibodies, please visit the Institute for Protein Innovation Antibody Collection webpage...Quality Control Each lot of antibody undergoes testing, either in-house or through outside labs, to confirm..., freezing at -80 °C, thawing on ice, and then testing for loss of potency against a non-treated control...recommended assays. If an antibody has passed stability testing, it may ship at room temperature. However, Addgene...

...HR700_TP53 Exon Mutation Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9...agreement between the recipient and the depositing institution(s). It governs the transfer of biological materials...distribute plasmids on behalf of the depositing institutions. These agreements indicate the language which... by an authorized signatory at the recipient institution. An authorized signatory is someone who can execute...execute legal documents on behalf of the recipient institution. Once the MTA has been signed, it can be submitted...

...defines 5 types and 16 subtypes based on shared characteristics and evolutionary similarity. These are grouped...easily packaged in AAV. Cas9 orthologs also have distinct PAM sites that do not cross react, allowing multiple...researchers to further develop the CRISPR toolkit by posting lab protocols , providing tips from experts in ...:1479-91. PMID: 24360272 Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg.... Science . 337(6096):816-21. PMID: 22745249 Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT...effects. Nature . 529(7587):490-5. PMID: 26735016 Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng...

... target genes or regulatory regions, where it stimulates transcription by recruiting activators or modifying...protocol for the best results. If you have any questions about the library protocols, feel free to contact...outline for using lentiviral gRNA libraries in postivie or negative screens. The use of appropriate controls...

...Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance...61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64...vector, CMV promoter, Hygro Campeau Viral Service Suggestions Don't see what you are looking for? Please let...viral format by filling out our Viral Service Suggestion Form (Link opens in a new window) . Please note...

...Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS), bearing either amino- or carboxyl-terminal...Neurodegenerative Disorders - Nicholas Cole Lab. A kit consisting of plasmids with four promoters, six fluorophores...resource center based in China focused on collecting existing zebrafish resources and developing new lines and...Center (EZRC) - A repository at the Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish...genomics in zebrafish. Return to top Do you have suggestions for other plasmids that should be added to this...

... plasmid tools generated by IGVF Consortium investigators to examine how genomic variation affects genome...phenotype. The National Human Genome Research Institute (NHGRI) is funding this collaborative program ...program that brings together teams of investigators who will use state-of-the-art experimental and computational... funded by the National Human Genome Research Institute (NHGRI) (Link opens in a new window) . Find IGVF...

...Mazzone CM, D'Agostino G, Halladay LR, Hardaway JA, DiBerto JF, Navarro M, Burnham N, Cristiano C, Dorrier...following resources may be of interest for groups interesting in implementing intersectional experimental design... in a new window) for the molecular design and testing of novel INTSRECT plasmids for groups interested...Wang W, Robertson K, Osten P, Sabatini BL. 2019. Distinct Cortical-Thalamic-Striatal Circuits through the...

...teaching different types of courses, at any type of institution, and at different points in their careers to ...inexpensive to assay, and easy to purify using histidine-tagged constructs and routine protein and molecular...facilitates scientific collaborations between institutions. With these plasmids, faculty can begin a sustainable...watermelon, Streptomyces ) Subcellular compartment (chloroplastic, cytoplasmic, glyoxysomal, mitochondrial, peroxisomal...

...plasmids Addgene distributes cannot be used to reconstitute self-replicating microbes or recreate the diseases...Synechococcus sp. Thermus sp. Vibrio sp. Yersinia pestis Plasmids for Fungi Aspergillus sp. Cryptococcus...community by depositing your useful tools , or by suggesting plasmids for the collection. Return to top Plasmid...opens in a new window) Return to top Do you have suggestions for other plasmids that should be added to this...

...Pathway Plasmids mTOR Gene List Resources The m echanistic or m ammalian t arget o f r apamycin (mTOR) is...known as STK11; serine/threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 PRAS40...Mitogen-activated protein kinase associated protein 1 mTOR Mechanistic target of rapamycin p53 TP53; tumor protein p53...nrd4505. PubMed PMID: 25743081 . Do you have suggestions for other plasmids that should be added to this...

...Parkinson's Christien Merrifield 27700 Hip1R-tDimerRFP-N1 Hip1R RFP CMV Parkinson's Christien Merrifield...Hurley 237478 pLCKO-CMV-TMEM106B-P2A-Blasticidin TMEM106B P2A, Blasticidin CMV Frontotemporal dementia in GRN...ClinVar) and is meant to be representative but not exhaustive. If you do not see a particular gene of interest...114410 AICSDP-64: FUS-mEGFP FUS GFP ALS Allen Institute for Cell Science 114601 pTWIN1-His6-Ssp-Htt18-...Ward 178171 SGP11_Halo_N_allele SGP11 Halo ALS, Spastic paraplegia Michael Ward 178172 TBK1_Halo_C_allele... pFB-EPCP1 LRRK2 His, Z, TEV polH Parkinson's Sebastian Mathea 227004 pAAV-CMVenh synapsin-intron-aSYNUCLEIN... pFB-EPCP5 LRRK2 His, Z, TEV polH Parkinson's Sebastian Mathea 228917 sg_Atm_i3-ipUSEPR-TR657 ATM TagRFP...

...includes titering, purity assessment, sterility testing, and viral genome sequencing (VGS). In-stock AAV...Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing Samples Small samples of control AAV vectors for...CAP-B10, CAP-B22 University of Florida Serotype Testing Panel for the Eye and Brain For enhanced transduction...

...interference (RNAi) experiments at Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA... silencing mechanism. It is a useful tool to investigate the roles of specific genes or to knockdown genes...retroviral and AAV backbone options. Plasmids for constitutive expression as well as those that allow for conditional...

...Phosphoprotein Kit S. cerevisiae Advanced Gateway Destination Vectors TAL Effectors Zinc Finger Consortium ...Link opens in a new window) — Joint Bio-Energy Institute Synberc (Link opens in a new window) — Synthetic...new window) — Open access journal Do you have suggestions for other plasmids that should be added to this...

...NPY2R Human Mouse IgG2a 177465 Anti-Collybistin [L120/30R] Collybistin Rat Mouse IgG2a 177466 Anti-nNOS/...Alpha1 Human Mouse IgG2a 177575 Anti-Collybistin [L120/12R] Collybistin Rat Mouse IgG2a 182096 Anti-Kv2.1...IgG2a 225365 Collybistin (Rattus norvegicus) recombinant monoclonal antibody. Collybistin Rat Mouse IgG2a...BAIAP2 Human Mouse 190507 Collybistin scFv [L120/12] L120/12 scFv Collybistin Rat Mouse 190508 Bassoon ...R-mAbs have been engineered such that they are a distinct IgG subclass from the conventional mAb to facilitate...

...) PDLP1 mCherry Maria Harrison 61200 pCMU-PLAr Plastids (Plants) Rubisco1 mCherry Maria Harrison 24898...-pmCherryC1 Actin filaments Cortactin mCherry Christien Merrifield 36201 pLifeAct-mTurquoise2 Actin filaments...structure of interest. Additional Resources Allen Institute for Cell Science Plasmid Collection Michael Davidson...items are updated more frequently. Do you have suggestions for other plasmids that should be added to this...

...NeuroMab/Trimmer Lab Recombinant mAb Collection Institute for Protein Innovation Antibody Collection Other...expression vector. bioRxiv 2026.03.18.712111. Institute for Protein Innovation (IPI) One-step assembly...Description Gene/Insert PI Return to top Do you have suggestions for other plasmids that should be added to this...

...community by depositing your useful tools , or by suggesting plasmids for the collection. Return to top Plasmid...on miniMos and MosSCI plasmids can be found on Christian Frøkjær-Jensen's Wormbuilder website. General ...organism C. elegans. Return to top Do you have suggestions for other plasmids that should be added to this...

... simplifies gene silencing and genome editing logistics. Return to top Reporter Plasmids The table below...fluorescent protein-based markers expressed from constitutive and arbuscular mycorrhiza-inducible promoters...items are updated more frequently. Do you have suggestions for other plasmids that should be added to this...

...credit: Maya Kostman for the Innovative Genomics Institute The Coronavirus Disease-2019 (COVID-19) pandemic...2019 novel coronavirus SARS-CoV-2 using CRISPR diagnostics: DETECTR (Link opens in a new window) SHERLOCK...last reviewed on 26 September 2025. Do you have suggestions for other plasmids that should be added to this...

...Genetics 2014 Allen Institute for Cell Science Plasmid Collection The Allen Institute for Cell Science has...and EGFP are available at Addgene. Do you have suggestions for other plasmids that should be added to this...

...changed the way researchers can explore important questions surrounding serine phosphorylation by adding this...improved phosphoprotein yield, rEcoli growth characteristics, and an overall improved ease of use when expressing...precise knowledge of phosphorylated positions for testing phosphoproteomics methods, proteomics search algorithms...

...them. Most SSR enzymes are categorized into two distinct families, serine recombinases and tyrosine recombinases...Expression of both fragments in the same cell reconstitutes a functional protein, allowing recombination...Content last reviewed on 31 July 2025. Do you have suggestions for other plasmids that should be added to this...

... the absorbance of a liquid you can accurately estimate the concentration of a substance in that liquid...

...construct from Alla Karpova at Janelia Research Institute....staining, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector ...vector preparation. In some cases, in vivo testing of these viral preparations was performed by the Karpova...

...spreading of antibiotic makes it easy for an investigator to conveniently plate and select transformed...

...the scale later on as otherwise, the paper could stick to the surface. Tare the weighing boat or paper....