...sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...viral genome, and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a...customizable DNA or RNA label for fluorescence microscopy. RNA aptamers, like MS2 or PP7, can also be fused to...to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can...dCas9 and S. aureus dCas9) tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal...imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent CRISPR systems have been used for dynamic tracking...

...measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely with... cells were labelled with a green or violet, fluorescent dye, respectively. WT and KO cells were mixed... specific detection of human TRPA1. Scientific Reports, 9(1), 18500. https://doi.org/10.1038/s41598-019...use in western blot, immunoprecipitation, immunofluorescence and flow cytometry. F1000Research, 13, 817...

... the commonly used cassettes, such as fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre, Flp... make. For example, large insertions, like a fluorescent protein knock-in, suffer from low efficiency ...with your desired insertion feature (e.g., a fluorescent protein), you can amplify the desired region ..., et al. "One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated...

...elements. These plasmids contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the...recognizes the different fluorescent labels. The machine detects which fluorescently labeled nucleotide is...the control of a non-bacterial promoter) or a fluorescent protein (that can be used to select or sort the...information on CRISPR check out Addgene’s CRISPR guide . Reporter Plasmids - Used for studying the function of genetic...relies on the random incorporation of modified, fluorescently tagged bases onto the growing DNA strand in ...process occurs when the polymerase incorporates a fluorescently tagged nucleotide. Because these special bases...nucleotide, the reaction is halted once the fluorescently tagged base is incorporated. Sanger sequencing...

...: Tet- or Cre-based regulation and fluorescent fusions or reporters. Browse lentivirus plasmids available...another marker, such as GFP. A researcher can use FACS to sort cells expressing GFP and later expand these...

...sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...viral genome, and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a...customizable DNA or RNA label for fluorescence microscopy. RNA aptamers, like MS2 or PP7, can also be fused to...to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can...dCas9 and S. aureus dCas9) tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal...imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent CRISPR systems have been used for dynamic tracking...

...optical switches Sensors are genetically-encoded reporters of molecular signals; e.g., calcium indicators...plasmids allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb and ePDZb1 as tags. pDawn/pDusk...activator FixJ in the absence of blue light. 470 Fluorescent Protein domains Dronpa based Light-inducible ...engineered from a green-to-red photoconvertible fluorescent protein 400 UVR8 domains UVR8 plant photoreceptor...

... as well as the type of transgene delivered: fluorescent proteins, optogenetic sensors, CRISPR elements...expression from mouse to primate neocortex. Cell Reports, 34(13). https://doi.org/10.1016/j.celrep.2021.108754...relatively even distribution of target gene expression (EGFP, in the depositing manuscript) across the brain....