... extreme sports enthusiast. He recently obtained his Ph.D. in neuroscience from the University of Rochester...inflammation and neurodegeneration. He now spends his time learning and writing about science whenever ...

...Northeastern University in Boston, MA. He obtained his BSc and MSc in Plant Biotechnology from Leibniz Universität... Universität Hannover (Germany), before starting his work in the Lee-Parsons Lab.     References D'Aoust...below. The GUS gene (β-glucuronidase) with histochemical GUS-staining is a very useful visual reporter...

.... So when Dave Westenberg taught this concept in his microbiology lab course, he decided to add a bit ...

...from John Chodera's Lab - Get high expression of His-tagged human kinases in bactera. Listen to Our Segment...

...E GAA, GAG Glycine Gly G GGU, GGC, GGA, GGG Histidine His H CAU, CAC Isoleucine Ile I AUU, AUC, AUA Leucine...Tag Amino Acid Sequence FLAG DYKDDDDK HA YPYDVPDYA His HHHHHH Myc EQKLISEEDL V5 GKPIPNPLLGLDST Xpress DLDDDDK...

...Matthijs Dorst’s Deck the Lab Nativity scene below his Christmas tree! It is that time of the year again...

...coverage on the subject in the New York Times and on his blog, where he has offered to answer readers' questions...

...in Molecular Plant Sciences since December 2020. His research focuses on the efficiency of transposon-... with low efficiency.       Sheng Yang obtained his Ph.D. from Shanghai Institute of Biochemistry, Chinese...

...member of the Addgene Scientific Advisory Board. His laboratory at Columbia University is focused advancing...the creation of innovative technologies. To date his group has develop several popular CRISPR-based tools...

...Scientist Jessica Welch showed some highlights of his travels from 2015, including a great European photo...

...Mammalian Sato 123128 pET-His-Cre 6xHis-Cre T7 Bacterial Liu 123131 pET-His-VCre 6xHis-VCre T7 Bacterial Liu...9xGGS-Cre-6xHis Cre fused to highly cationic GFP variant T7 Bacterial Liu 62939 pET-Cre-6xHis Cre-6xHis T7 Bacterial...Nxph4 Mammalian Zeng 62372 pET-(-30)GFP-9xGGS-Cre-6xHis Cre fused to supernegatively charged GFP variant...; Targeting vector for Pax7 Tanaka 111187 pBADZ-HisCre Cre; arabinose inducible. PBAD promoter Bacterial...

...minorities to study STEM subjects. Here, Sadangi and his team answer a few questions about The Addictive Brain...

...school, I came across Joseph Campbell, and read about his "follow your bliss' mantra and never forgot it. Usually...

...methods for cellular imaging with ultrasound. In his research he applies naturally occurring bacterial...

...out our interview with Michael Koeris to learn how his company uses phage to test for Listeria in food products...surpassed 60,000 views for the month of September! Historically we do better in September than in the summer...

... the Genetic Perturbation Platform at the Broad. His mom always told him that he has the best words and...

...techniques researchers are using. The detailed results of his survey can be found in the full post but TLDR: many...

...supplement of this paper), help each person organize his or her thoughts to facilitate honest conversation...

...not just to share your riches but to reveal to him his own." -Benjamin Disraeli Mentor, Sponsor, Advisor...

..." He successfully transitioned to industry after his PhD and a couple of postdocs focusing on molecular...

...the National Academy of Sciences in March, he and his colleagues took another important step forward by...

...development, teaching, etc. Church also encourages his mentees to follow their passion. “There are many ...

...guest blogger Robert Orr! Robert Orr recently earned his Ph.D. in Biology and Biotechnology from Worcester...polarized cell growth in the lab of Luis Vidali. For his next adventure, he will join the lab of Darren Gilmour...technologies, one might incorrectly relegate RNAi to history. The straightforwardness of RNAi is unmatched—unlike...cannot be used to investigate essential genes. Historically, gene silencing methods were thought to be afflicted...

...Communication where he completed a master's degree in 2020, his research explores risk-taking behavior among collision...

...or setting up commercial and nonprofit companies. His lab focuses on gene therapy work for genetic diseases...

...segment Similarly to the Carr lab, Erik Sontheimer and his group from the University of Massachusetts Medical...

...under a lot of pressure recently which had affected his judgement. Eventually, we published the paper in ...

... in the scientific aspects of writing. Following his advice, I applied to the class. Receiving the email...

...Additionally, I would like to thank Le Cong for all his hard work creating amazing resources for the scientific...your HR by doing Restriction Fragment Length Polymorphism (RFLP) (see Figure 4 of the Cong, et. al, 2013...

...Takeshi Imai is a professor at Kyushu University. His lab has been studying functional and anatomical aspects...

...academic journal for exactly the critiques leveled at his CCR5 project. It was recognized as an unethical experimentation...to argue otherwise). To be sure, throughout the history of medical research, there have always been people...

... and conceived SnapGene because he needed it for his own lab.     Aline Glick is VP of Product Management...

... a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter known to bind to the HMO of...

...solving critical sequencing problems. He received his PhD in Bioinformatics from Boston University, and...quick turn-around time of Sanger sequencing have historically matched the needs of most molecular biology ...

...steps to fluorescently tag a C. elegans protein. The his-72 locus is shown as an example. Used with permission...

...he directs the Nikon Imaging Center. He received his PhD in biophysics from UCSF in the laboratory of ...

...Division of Life Sciences at Rutgers University. His dissertation and research background is in citizen...

...team used nickel-coated agarose beads coated with his-tagged OptoNBs. OptoNB coated beads were then imaged...

...the Ragon Institute of MGH, MIT, and Harvard, and his lab found that the neutralization effectiveness of...

...diseases, Alzhiemer’s disease (AD).  Troy Rohn and his lab are researching a way to treat AD using CRISPR...

..." He successfully transitioned to industry after his PhD and a couple of postdocs focusing on molecular...

...bacteria interact with each other and plant surfaces. His system of choice is the Arabidopsis thaliana - Pantoea...serotonin receptor to study how single nucleotide polymorphisms present in the human population can affect drug...

...ComSciCon and Chair of its Leadership Team.  Nathan did his PhD in astronomy and works as a data scientist in...communities gather at ComSciCon's local and specialized franchise workshops throughout the year.  Find workshops...

...our guest blogger Jon Chow. Jon Chow is finishing his immunology PhD at Harvard University. He has had ...

...functions as one of the “healthy human volunteers” in his studies! This was followed by a talk from Professor...Immunosurveillance and Defense With increasingly sophisticated visualization techniques it is possible to follow...

... can be found climbing mountains or playing with his two cats. Keira Havens grew up in Hawaii where she...

...the kit now makes possible, and how it compares to his CRISPR/Cas9 system. Addgene: For people unfamiliar...modeling in stem cells. Addgene: Tell me about the history. How had investigators done this kind of work before...

...traditional “one at a time” approach. According to his math, it takes five hours of hands-on time to make...

...   About the author: Benedict Diederich received his PhD from the Heintzmann Lab at the Leibniz IPHT Jena...

..., courtesy of Addgene depositor Stephen Elledge. His new method, named sequence- and ligation-independent...