Skip to main content
Addgene
Showing: 101 - 134 of 134 results

  12. Genetic Code Expansion

    Type
    Collection
    ...Ryan Mehl 85496 pDule-Tet2.0 Tetrazine2.0 tRNA synthetase M. jannaschii Tetrazine 2.0 Bacterial TAG Ryan...Ryan Mehl 85497 pDule2-Tet2.0 Tetrazine2.0 tRNA synthetas M. jannaschii Tetrazine 2.0 Bacterial TAG Ryan...Bacterial TAG Ryan Mehl 164580 pUltraI-Tet3.0[TAA] Tet3.0RS M. barkeri Tet3.0 Bacterial TAA Ryan Mehl 172482 ...TAG Ryan Mehl 174080 pDule-Tet3.0 Tet3.0 tRNA synthetase M. barkeri Tetrazine 3.0 Bacterial TAG Ryan Mehl...NES-Flag-R2-84-MbRS Tetrazine3.0 NES-Flag-R2-84 tRNA synthetase M. barkeri Tetrazine 3.0 Mammalian Ryan...aminoacyl-tRNA synthetase M. jannaschii 1,2,4,5-tetrazine ncAAs Bacterial TAG Ryan Mehl 217361 pIDTSmart-MbPylRS...

  13. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...retrograde transsynaptic transfer of a protein, tetanus toxin, subsequent to its retrograde axonal transport...

  14. Bacterial Expression Systems

    Type
    Collection
    ... pdCas9-bacteria 44249 pTetO Anhydrotetracycline Stanley Qi Anhydrotetracycline inducible expression of... light. pCph8 50552 pLtetO-1 Anhydrotetracycline Jeffrey Tabor Anhydrotetracycline inducible expression... Plasmids pBAD, OR2-OR1-PR, pLtetO, pLlacO Arabinose, Anhydrotetracycline, lactose, IPTG Richard Murray...pwtCas9-bacteria 44250 CRISPR Stanley Qi Anhydrotetracycline inducible expression of wild-type Cas9 from...

  15. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...sensors, ER-targeted CEPIA1er ER-mitochondria tethering by PDZD8 regulates Ca(2+) dynamics in mammalian..., reagentless biosensor for ADP based on tetramethylrhodamine-labeled ParM A fluorescent, reagentless ...reagentless biosensor for ADP based on tetramethylrhodamine-labeled ParM. ACS Chem Biol. 2010 Apr 16;5(4):415-25...single cells. Angew Chem Int Ed Engl. 2018 Jun 27. Tetsuya Kitaguchi ATP Variants of ATP sensor iATPSnFR1.0...dual-color imaging. PLoS One. 2014 Jun 24;9(6):e100252. Tetsuya Kitaguchi cAMP (cyclic AMP) Red fluorescent protein-based...in vivo imaging. Sci Rep. 2017 Aug 4;7(1):7351. Tetsuya Kitaguchi cAMP (cyclic AMP) Fluorescent sensor ...and PDE5alpha. ACS Sens. 2017 Jan 27;2(1):46-51. Tetsuya Kitaguchi cGMP (cyclic GMP) FlincG3 (GFP-based ...

  16. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...eqFP611 559 611 35 Tetramer eqFP611-N1 - Mammalian Expression DsRed2 563 582 24 Tetramer pCAG-DsRed2 - Mammalian...CYPet-C1 - Mammalian Expression AmCyan1 453 486 11 Tetramer AmCyan1-N1 - Mammalian Expression MiCy (Midoriishi-Cyan...Expression (cysteine-free SGFP2) ZsGreen 493 505 39 Tetramer pHIV-Zsgreen - Mammalian Expression (this is a...YPet-pBAD - Bacterial Expression ZsYellow1 529 539 8 Tetramer ZsYellow1-N1 - Mammalian Expression mPapaya1 530...Expression Kaede 508 / 572 518 / 580 87 / 20 5.6/5.6 Tetramer Kaede-N1 - Mammalian Expression Kaede-C2 - Mammalian...

  17. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TTGAGGACCATGACGTGCagctggagactgaagaGAGCAAGAAAGTTGGGAA tet2(ENSDART00000113020) TAL3376 & TAL3377 TCAAGACCAGATACTATCcccaagcttcctttccCAGCTACCACCTACTGAA...TCAAGACCAGATACTATCcccaagcttcctttccCAGCTACCACCTACTGAA tet3(ENSDART00000137355) TAL3378 & TAL3379 TTGCTCCTTGGTTGCATCtctgtcttcattatcaCAAACATTTTCTTCATTA...TTGCTCCTTGGTTGCATCtctgtcttcattatcaCAAACATTTTCTTCATTA tetmethylcytosinedioxygenase 1 TAL3572 & TAL3573 TCAACTCGCGCATCCACAaagcgaaatgttaagaGGGTGAAGGCTTCCATGA...TCAACTCGCGCATCCACAaagcgaaatgttaagaGGGTGAAGGCTTCCATGA tetmethylcytosinedioxygenase 3 TAL3574 & TAL3575 TTATTAGGCACTTTTTTGtaaacgctgtattccaTGCACTTTCCTGTCCACA...TTATTAGGCACTTTTTTGtaaacgctgtattccaTGCACTTTCCTGTCCACA tetmethylcytosinedioxygenase 3 (previous si:ch211-220a11.1) TAL3576...

  18. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...-5aa linker-Cerulean-6aa linker-Venus ACAV Heterotetrameric construct consisting of Amber-5aa linker-Cerulean...linker-Cerulean-5aa linker-Amber-6aa linker-Venus ACVA Heterotetrameric construct consisting of Amber-5aa linker-Cerulean...linker-Cerulean-5aa linker-Venus-6aa linker-Amber VCAA Heterotetrameric construct consisting of Venus-5aa linker-Cerulean...linker-Cerulean-5aa linker-Amber-6aa linker-Amber VCVV Heterotetrameric construct consisting of Venus-5aa linker-Cerulean...

  19. Qi Lab CRISPR Page

    Type
    Collection
    ... plasmid ( pdCas9_bacteria ) contains an anhydrotetracycline (aTc)-inducible dcas9 gene on a p15A vector...targeting endogenous TEF1 promoter 46923 pSNR52-sgTET Yeast CEN/ARS vector (Ura3) that contains sgRNA ... promoter, targeting endogenous TRE elements of pTET07 promoter...

  20. Zhang Lab CRISPR Page

    Type
    Collection
    ...sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stemloop 2 The MS2-P65-HSF1 activation helper...61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop - Contains BbsI sites for insertion...lentiviral sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker....

  21. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...188572 Tet-off TauRD (P301L/V337M) MAPT Myc TRE Parkinson's, FTD Franz-Ulrich Hartl 188573 Tet-off Full...Ataxia Richard Youle 176485 Piggybac-TetO-hNurr1-blast NR4A2 TetO Parkinson's Marius Wernig 176852 UBQLN2...Ronald Hart 190811 pTet-O-APOE-E2-T2A-Puro APOE UbC Alzheimer's Ronald Hart 190812 pTet-O-APOE-E3-T2A-Puro... CMV Ataxia telangiectasia Stephen Elledge 43918 tetO-ALN NR4A2 His, V5 TRE Parkinson's John Gearhart ...T2A-Puro APOE UbC Alzheimer's Ronald Hart 190813 pTet-O-APOE-E4-T2A-Puro APOE UbC Alzheimer's Ronald Hart ...

  23. TALEN Plasmids and Kits

    Type
    Collection
    ...comprised of 834 plasmids of pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly....and Chad Cowan Ekker Lab TALEN Kit All possible tetramers (264) of RVDs, compatible with Golden Gate TALEN...

  24. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...methylation by DNMT3A or MQ1 cytosine demethylation by Tet1 These modifications persist over time and are potentially...

  25. AAV Viral Preps

    Type
    Collection
    ...Spagehetti Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell...

  26. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Publication 64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-1 Fujii Efficient isolation of specific genomic...

  28. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...p-bacteria Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify...

  29. CRISPR Guide

    Type
    Collection
    ...- activation through increased H3K27 acetylation TET1 - activation through cytosine demethylation DNMT3A... a companion CRISPRon to undo repression using a TET1-dCas9 fusion. Figure 10: Overview of epigenetic ...

  30. Deisseroth INTRSECT Collection

    Type
    Collection
    .... PubMed (Link opens in a new window) Wick ZC, Tetzlaff MR, Krook-Magnuson E. 2019. A novel population...

  31. Luciferase Plasmid Collection

    Type
    Collection
    ...enchancer regions in Drosopholia Herman Wijnen 126033 pTETRIS-cargo Firefly For use as a reporter of the ability...

  32. Molecular Biology Reference

    Type
    Guide
    ...England Biolabs F' proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (TetR) ∆(ara-leu) 7697 araD139 fhuA ∆lacX74 galK16 galE15.../mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL Tetracycline 10 mg/mL 10 µg/mL *Note: Carbenicillin can be...

  33. CRISPR Guide

    Type
    Guide
    ...- activation through increased H3K27 acetylation TET1 - activation through cytosine demethylation DNMT3A... a companion CRISPRon to undo repression using a TET1-dCas9 fusion. Figure 10: Overview of epigenetic ...

  34. Promoters

    Type
    Guide
    ...from phospholycerate kinase gene TRE Inducible Tetracycline response element promoter U6 Constitutive Human...
Showing: 101 - 134 of 134 results