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Showing: 101 - 150 of 535 results

  1. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...protocols, and, of course, plasmids. Today we’re trying a new way to present plasmid info with a new segment...

  2. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    ...great place to start if you’re new to the topic. Or, try the search bar Now, you can search for the articles...

  3. Kit Free RNA Extraction

    Type
    Protocol
    ...instead of incubating at -20°C for 1 hour, you can try incubating at -80°C overnight. Centrifuge for 20 ...

  4. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...your data, too!   Using the Data Hub If you're trying to decide which AAV is right for your experiment...

  6. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ... The PAM sequence is of particular concern when trying to edit a gene using homology directed repair, ...of reagents available through Addgene! If you're trying to decide which CRISPR tools are best for your ...

  7. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...taking a look at what works and what doesn’t, and trying all over again. Here’s that process!   Figure...Shot without anyone else in the office.    I try to build every setup with an eye to the future. I...

  9. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...to probe interactions in locations of interest.  Try BioID for your proximity labeling experiment! APEX...favorite protein or RNA motif, give these methods a try.  References:  Branon, Tess C., et al. "Efficient...antibody based affinity purification and mass spectrometry approaches. Recently however, scientists have...classic antibody based affinity purification/mass spectrometry methods.  BioID: a promiscuous biotin ligase...purified using streptavidin and separated via flow cytometry.  Find plasmids for EXCELL here! Since BioID was...

  10. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...lentiviral preps unless absolutely necessary. Many labs try to use freshly collected lentivirus for downstream...well to other cell types. To overcome this issue, try transducing with a range of dilutions of your lentiviral...detection kits that use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels...

  11. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ... associations across different universities are trying to compile more comprehensive demographic information...beyond those required at the lab bench. At Addgene we try to do our part in boosting awareness of various career...

  18. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ... to have as much context as possible. When I am trying to resolve a conflict between two different teams... of conflict resolution. In compromise, you are trying to come to a consensus that all conflicted parties...

  19. A Control for All Seasons

    Type
    Blog Post
    ... knockout.  If knockout samples are unavailable, try substituting with knockdown approaches using siRNA...weak, then it is best to reassess the protocol and try to find a way to reduce, if not eliminate, the noise...W. “Controls for Immunocytochemistry.” Journal of Histochemistry & Cytochemistry, vol. 59, no. 1, ...al. “Controls for Immunohistochemistry.” Journal of Histochemistry & Cytochemistry, vol. 62, no. 10...that the target protein was measured on.    Flow cytometry In addition to the controls listed above, viability...compensation controls are recommended for flow cytometry. A viability control allows you to detect dead...fluorophore and are critical to analyze multiplex flow cytometry experiments. We hope that this blog post will ...

  20. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...GATC) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes (ex: ClaI...CCWGG) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes that are...

  21. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...for career skills. Download a summary PDF here or try out the online Researcher Development Framework Planner...lab that has multiple collaborations, even with Industry labs Work on a big project that relies on a division...inviting others (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the ...

  23. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...freezer. No more issues deciphering handwriting or trying to find to where the binder was moved. You’ll have...and you can’t remember where you put your flow cytometry protocol. Or maybe you can’t find the molecular...

  24. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...growth after overnight incubation. What went wrong? Try growing the culture for more time. Some bacterial...following into a 500 mL glass bottle: 4 g NaCl 4 g Tryptone 2 g Yeast Extract and dH 2 O to 400 mL Note: If...

  25. Validated gRNA Sequences

    Type
    Collection
    ...GAGTCCGAGCAGAAGAAGAA 47508 cut S. pyogenes 23792628 Joung ETC2, TRY, and CPC A. thaliana 71288 cut S. pyogenes 26193878...

  26. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...one you use, of course, depends on what you are trying to do – there’s no “best” tool among them. While...best” gRNA depends an awful lot on what you are trying to do: gene knockout, a specific base edit, or ...Rees HA, Liu DR (2018) Base editing: precision chemistry on the genome and transcriptome of living cells...

  27. Antibodies 101: Validation

    Type
    Blog Post
    ...sensitivity, which can be problematic if you are trying to show that your antibody can distinguish between...Additional resources on the Addgene blog:  Still trying to choose an antibody? Check out our Selecting ...This is a good option for applications like immunohistochemistry on human tissues, when (1) other strategies...correspond to protein levels, so you perform immunohistochemistry with your antibody on these tissues (B)....recognized by your antibody. You perform immunocytochemistry on these mutant cells as well as a non-mutant...epitopes of this protein.   You perform flow cytometry using both antibodies, as well as a third unrelated...sample using the antibody and then uses mass spectrometry to identify the captured proteins. If the antibody...

  28. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...unpublished evidence shows otherwise. Others will go on to try to apply these findings in their own work ultimately...likely that we’ll find a fluke when we’re simply trying to get our experiments to work, but part of good... sciences and featured speakers from academia, industry, nonprofits, and publishing. The livestream video...publishing community views reproducibility, how industry relies on reproducibility, and how you can push...

  29. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...secondary antibody may be binding non-specifically – try optimizing your blocking and washing conditions or...are always new and improved variations you could try. We hope this post helped you start planning for ...referred to as immunocytochemistry (ICC) when the sample is cultured cells or immunohistochemistry (IHC) with....3001161 McLaughlin, K. (2019). Multiplexing Immunohistochemistry. Materials and Methods, 9. https://doi.org...antibodies available at Addgene Protocols for Immunocytochemistry Protocols for other antibody applications...

  30. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...or two hanging out around the lab and especially try to attend a lab meeting. Observe how the lab members...scientific meetings (and present)? Making the decision Try to rotate or interview in more than one lab when ...(topic and/or technical aspects) applicable to industry if this path interests you  (e.g. human disease...drug/device development? Does the advisor have industry connections such as serving on Boards? Does the...the publication record show evidence of industry collaboration? If you are considering a career at a small...

  32. Science as Art

    Type
    Blog Post
    ...in an Open Source Lab Resources on Addgene.org Try the Plasmid Land Game ...

  38. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ...with high marker expression level. Labs have been trying different approaches to improve CTC capture, but... is small, highly stable and has this amazing chemistry locking the domain together - no one knows what.... Many other Gram-positive bacteria have this chemistry in their surface proteins. It is best studied ...

  39. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...fewer and fewer people use plastic bags.  We also try to recycle our discards according to the Cambridge...plastic materials are fairly inexpensive in this country and we are quick to throw out chipped or imperfect...

  40. Antibody Guide

    Type
    Collection
    ...select a new antibody using the above process and try again. In some cases, it may be preferable to buy...Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting - used to...immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (ICC) use labeled antibodies... one protein of interest. Immunohistochemistry (IHC) and immunocytochemistry (ICC) IF can refer to both...both immunohistochemistry and immunocytochemistry when fluorescent signaling molecules (also called dyes...Applications - Cell Sorting Methods Flow cytometry Flow cytometry, used commonly in immunology, allows you...capture, and sort single cells. Includes: Flow cytometry Read on to learn more about the applications common...

  42. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ... the protein as it exists in your assay?  First, try to understand, to the best of your ability, what ...protein that is being used as a marker for flow cytometry, for example, can only be recognized by antibodies...samples. If, however, you are performing an immunohistochemistry assay, you may want to rule out monoclonal...

  46. Hot Plasmids - October 2022

    Type
    Blog Post
    ...actively testing it in other applications! If you try it out in a new assay, let us know. We’d love to ...
Showing: 101 - 150 of 535 results