We narrowed to 46 results for: ACL

...system from Michael Bassik’s lab, overcomes this obstacle. With CRISPR-X, dCas9 is used to recruit an sgRNA...

... in annealing buffer (10mM Tris, pH7.5-8.0, 50mM NaCL, 1mM EDTA) and mixed in equimolar concentrations...

...out the following into a 500 mL glass bottle: 4 g NaCl 4 g Tryptone 2 g Yeast Extract and dH 2 O to 400...

...of recombinant DNA technology has overcome this obstacle by enabling the preparation of more manageable...

...polybrene (Sigma-Aldrich catalog #H9268) in 0.9% NaCl. Autoclave to sterilize. Stock solution is stable...

...elements are needed. One strategy to overcome this obstacle, has been to use smaller Cas9 orthologs, such ...