...Glycol 8000 (PEG) + 0.5 M NaCl Cell lysis buffer (50 mM Tris HCl, 150 mM NaCl, 2 mM MgCl2) Reagent Preparation...solution: Dissolve 400 g of PEG 8000 and 24 g of NaCl into deionized water and adjust to a final volume... °C. Cell Lysis Buffer : 50 mM Tris HCl, 150 mM NaCl, 2 mM MgCl 2 Add the following to the 2 L sterile...

...system from Michael Bassik’s lab, overcomes this obstacle. With CRISPR-X, dCas9 is used to recruit an sgRNA...

...purposes of isolating one specific DNA sequence. This obstacle has been overcome by the field of recombinant ...

... in annealing buffer (10mM Tris, pH7.5-8.0, 50mM NaCL, 1mM EDTA) and mixed in equimolar concentrations...

...out the following into a 500 mL glass bottle: 4 g NaCl 4 g Tryptone 2 g Yeast Extract and dH 2 O to 400...

...polybrene (Sigma-Aldrich catalog #H9268) in 0.9% NaCl. Autoclave to sterilize. Stock solution is stable...