We narrowed to 46 results for: ACL

1. 27 Hot Plasmids from 2016

   Type

   Blog Post

   ...system from Michael Bassik’s lab, overcomes this obstacle. With CRISPR-X, dCas9 is used to recruit an sgRNA...

2. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

   Type

   Protocol

   ... in annealing buffer (10mM Tris, pH7.5-8.0, 50mM NaCL, 1mM EDTA) and mixed in equimolar concentrations...

3. Protocol - How to Inoculate a Bacterial Culture

   Type

   Protocol

   ...out the following into a 500 mL glass bottle: 4 g NaCl 4 g Tryptone 2 g Yeast Extract and dH 2 O to 400...

4. Protocol - pLKO.1 – TRC Cloning Vector

   Type

   Protocol

   ...polybrene (Sigma-Aldrich catalog #H9268) in 0.9% NaCl. Autoclave to sterilize. Stock solution is stable...

5. Molecular Biology Reference

   Type

   Guide

   ...purposes of isolating one specific DNA sequence. This obstacle has been overcome by the field of recombinant ...

6. Adeno-associated virus (AAV) Guide

   Type

   Guide

   ...elements are needed. One strategy to overcome this obstacle, has been to use smaller Cas9 orthologs, such ...