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Showing: 41 - 60 of 1087 results

  1. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...Addgene blog Antibodies 101: Introduction to AntibodiesAntibodies 101: IsotypesAntibodies 101: Chimeric...secondary antibodies to bind. We also know that changing the Fc domain can impact an antibody's binding ... affect antibody applications like ELISAs or Western blots, they are key in helping antibodies drive an...responses? We got you! You can also review antibodies in our Antibodies 101 animation.)  Many types of immune...receptors that bind to the Fc domain of an antigen-bound antibody and induce (or, in the case of the Fc receptors...a viral infection a) antibody-dependent complement deposition (ADCD) b) antibody-dependent cellular cytotoxicity... et al., 2019.    Antibody-dependent complement deposition (ADCD) Antibodies can bind to a pathogen...

  2. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...Addgene.org Addgene’s General Use Antibodies Antibody guide Antibody protocols Additional resources ...of finding a ‘good’ antibody is something we’ve all experienced. Finding an antibody that works for your... common tags, the antibodies used to detect them, and how they can fix your antibody woes.   What are...without the need for an antibody. Tags can enable the separation of detection of a mutant protein vs. endogenous...monoclonal antibodies. Below we review the most commonly available tags and their antibodies along with...Addgene blog Antibodies 101: Epitope availability Plasmids 101: Protein Tags Antibodies: Of Myc and Men...Epitope tags eliminate the need for target-specific antibodies and have been widely used across species and ...

  3. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post
    ...single antibody to visualize their target. These antibodies are generated against a specific antigen and ...two antibodies: a primary and secondary. For indirect IF, the primary antibody targets the antigen of ...each antibody performs differently and some antibodies impact the performance of other antibodies. But...secondary antibodies on hand to mix and match with a larger collection of primary antibodies. This strategy...target and sample type. Primary Antibody This is the first antibody applied to your sample, which will...incubation time. Secondary Antibody Unsurprisingly, this is the second antibody you apply, which binds ...experimental context.  Choosing antibodies for your assay The antibodies you choose for your experiment...

  4. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...expression, measured using antibody conjugated fluorophores Fluorescent antibodies get around the challenges...staining a primary antibody binds to a target and a fluorochrome-conjugated secondary antibody binds to the ...are low as multiple secondary antibodies can bind to a primary antibody thus amplifying the signal.   ...should also include a secondary antibody control in which the primary antibody has been omitted from the staining... about antibodies Resources on Addgene.org Visit the Fluorescent Protein Guide Find antibody plasmids...Have you ever wanted to measure expression of your protein of interest in a single cell? Or perhaps, ...surface receptor are stained with a fluorescent antibody against the receptor and analyzed on a flow cytometer...

  5. Corralling and Cornering the Goldilocks Antibody

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    Blog Post
    ...their recombinant antibodies. Since recombinant antibodies are a newer technology, it is important to show... blog Antibodies 101: ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies    ...and cost-effective) antibody for my experiment – the “goldilocks antibody.” Antibodies are one of the most... the antibody crisis, one must corral and corner the goldilocks antibodies. A goldilocks antibody is highly... popular and useful antibodies are, they are in a midst of a crisis. The “antibody crisis” came to light...light as antibodies became commercialized, revealing the unreliability of antibodies. There are three main...ability – i.e., the antibody can no longer bind to the target). Unreliable antibodies cause research that...

  6. Antibodies 101: Affinity Reagents

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    Blog Post
    ....org  Antibody GuideRecombinant antibodies available from AddgeneBrowse our recombinant antibody plasmid...as is antibody recognition, since antibody-specific antibodies bind to and recognize the constant region... (the antibody region that binds to the antigen) from existing antibodies. Each cleaved antibody will ...from camelid antibodies to antibody fragments to tools completely unrelated to antibodies.  Why use an...co-crystalize, a major advantage over antibodies (Kwon, 2019).  Single-domain antibodies (sdAbs), sold under...colors.  To antibody or not to antibody?  That is the question… or is it? This post isn’t meant to convert...101: Aptamer FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction to Antibodies Additional resources...

  7. Our New Antibody Mascot is....

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    Blog Post
    .... Name Our New Antibody Friend We need your help naming our latest mascot, an antibody joining us on ...following along on our journey to name our new antibody mascot, who's joining us in preparation for our...for Abi as they help us prepare for our upcoming antibody launch in late March!      *If you submitted ...Blugene and Aavery's newest friend, our Addgene antibody mascot, here to help us with our upcoming affinity...suggestions here or by using the hashtag #AntibodyMakesThree on Twitter. As with Blugene and Aavery, we...

  8. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...s blog Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction...Introduction to Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene...collection of antibodies in your lab. Perhaps you’re even in that mythical place where antibodies are well-...hides the epitope that the antibodies used to recognize the protein, your antibody will no longer be effective...immunohistochemistry assay, you may want to rule out monoclonal antibodies that have been validated solely...Validation  Once you’ve selected an antibody, or multiple antibodies, you’ll need to validate them for ...which antibodies are suitable for which assays and conditions when you’re building your antibody library...

  9. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...library to a significant degree using different types of AAV. Expression Another advantage of AAV vectors...ones. In those cases, AAV vector screens may be AAVantageous!       In vivo, in vitro, ex vivo CRISPR screens...cells with the CRISPR library in vitro before transplanting them into mice. For in vivo screens, mice are...locations that could cause deleterious effects. It is important to note, however, that while AAV rarely integrates...the amount of cloning, another option is to use variants of Cas9, like split Cas9, or a smaller Cas9 ortholog...Laboratory). https://doi.org/10.1101/2023.06.13.544831 Santinha, A. J., Klingler, E., Kuhn, M., Farouni, R., Lagler...

  10. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...Resources on Addgene.org Addgene antibodies Addgene’s antibody guide Antibody protocols  Resources on the...especially if downstream antibody detection is desired… and yes, we have an antibody for that!   Fig....of these tags also have commercially available antibodies, resins, beads, and other purification aids readily...are trying to purify The expression system If you want the tag to remain on your protein for downstream... to remove the cleaved tag from solution. MBP antibodies are available for detection and/or purification...purification of MBP-tagged proteins. The antibody is an alternative to amylose resin and also provides a visual...you are having solubility issues!). Need a GST antibody? Addgene has you covered! His His tags, often ...

  11. Luminescent Imaging with Nano-lanterns

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    Blog Post
    ... developed Nano-lantern technology. Nano-lanterns contain a Renilla luciferase variant fused to an FP;...contexts. Improving Nano-lanterns In 2015, Takai et al. expanded the Nano-lantern color palette to include...producing a constant supply of substrate. Using this compound to excite the Nano-lanterns, Takai et al... Nano-lanterns (eNL) described in Suzuki et al. improve the brightness of previous Nano-lanterns by 2-...appropriate for use with Nano-lantern technology. Multicolor imaging using Nano-lanterns requires a linear unmixing... signals of various Nano-lanterns, but it does not isolate each Nano-lantern, so Takai et al. used a linear...explained further in Takai et al. Nano-lanterns and ready-made Nano-lantern biosensors are available from Addgene...

  12. 3 Challenges in Plant Synthetic Biology

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    Blog Post
    ...limited understanding of plant biology is a significant technical obstacle to plant synthetic biology. We...pigments (anthocyanins), and the plant circadian clock. Petunias have been a model organism for plant research...here. The first transgenic plant was engineered over 30 years ago, but plant synthetic biology is still...because working with plants can be challenging, there are a lot of unexplored areas in plant biotechnology that...themselves if they want this beautiful flower in their front yard or not. By developing plant aesthetics with...pivot towards plant biotechnology and provide a much needed boost to basic molecular plant research, more...the lack of resources associated with plant synthetic biology. Plant molecular biology has traditionally...

  13. Six Spooky Science Stories and Halloween at Addgene

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    Blog Post
    ...science stories Zombie ants Ophiocordyceps unilateralis is a fungus that infects ants and controls their ...atrophy. After the ant’s death, the fungal fruiting bodies being to sprout out of the ant’s body. The fruiting...their behavior. These ants become “zombies” and leave their foraging trail. They climb upwards until they ...temperature and humidity favors fungal growth. The ants bites down and locks their jaws into a leaf, and... onto the forest below for the next unsuspecting ant to stumble upon. Vampire bacteria Vampirovibrio chlorellavorus...genes were appropriately named as spook, spookier, phantom, disembodied, shadow, and shade. Tongue-eating ...

  14. Antibody Data Repositories and Search Engines

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    Blog Post
    ... validated antibody, we recommend using antibody search engines and antibody data repositories to find... that can help: Antibody data repositories, which share validation data, and antibody search engines, ...many different antibody catalogs at the same time.  If you're looking for an antibody, especially a validated...improving antibody quality throughout research, consider checking out the Only Good Antibodies community...If you’re looking for an antibody that works for your experiment, you may be overwhelmed by options —...find an antibody for your experiment. Search engines let you easily find and compare available antibodies...and experimental data to help you decide if the antibody is a good fit for your experiment. They come in...

  15. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    ...looking to use antibody-based applications in their research.  Like all our eBooks, the Antibodies 101 eBook...101 blog posts on an antibody-specific topic, allowing you to easily access relevant information for planning...re very new to antibodies as research tools, you may find “Chapter 1: What is an Antibody” quite useful...to find an antibody that works, we highly recommend “Chapter 3: Finding the Right Antibody for Your Experiment...Experiment.” Whatever your antibody application question, the Antibody 101 eBook is a great place to start...excited to share the very first edition of our Antibodies 101 eBook! The latest addition to our eBook collection...

  16. Antibodies 101: Beyond Surface Labeling

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    Blog Post
    ...Ready-to-use recombinant antibodies at Addgene Addgene's Antibody Guide Addgene's Antibody Plasmid Collection...Addgene blog Antibodies 101: Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...single-fluorophore-conjugated antibodies. These methods can include labeling markers with multiple proteins/antibodies, targeting... to antibodies and 'staining' to refer to small molecule dyes. Indirect labeling While antibodies used...fluorophore-conjugated antibody commercially available. In other cases, fluorophore-conjugated antibodies may exist...fluorophore-conjugated secondary antibody is added that binds the conserved, host species-specific (“anti-rabbit”) region...region of the primary antibody (Figure 1b). As above, the advantage of this system is increased flexibility...

  17. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ... from your antibody panel. They are made up of compensation beads and a conjugated antibody. Compensation...your sample. The isotype antibody will be virtually identical to the antibody for your marker of interest...Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Conventional vs. Spectral...Resources on Addgene.org Addgene's Antibody Guide Browse Addgene's Antibody Collection        ...Depending on the markers you are looking at and the antibodies you are using, you may need to include additional... synthetic beads that can bind to a conjugated antibody and beads that are unable to bind to them: like...The compensation beads can bind to any primary antibody you add. Single color controls are used to help...

  18. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... IPI anti-integrin toolset IPI’s anti-integrin toolset comprises 26 recombinant antibodies (rAbs) either...Addgene's Antibody Guide Antibodies 101: Flow Cytometry Antibodies 101: Introduction to Antibodies...Table 1: IPI antibodies  Antibody Target Proteins Reactivity Isotype Source Species Anti-Integrin...unique antibodies that bind discrete subunits or particular subunit pairs. Some of the antibodies in the...property in an antibody is extremely rare. While many function-blocking integrin antibodies exist, almost...are chimeric antibodies composed of rat or mouse variable regions fused with rabbit constant domains. All...monoclonals, we anticipate that our chemically identical hybridoma-derived antibodies will also work in...

  19. Antibodies 101: Choosing the Right Isotype

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    Blog Post
    ...Addgene The Antibody Guide Antibodies 101: Isotypes Antibodies 101: Choosing the Right Antibody  ...polyclonal antibody is better for your experiment. Monoclonal antibodies contain a single antibody clone and...anti-mouse antibody. Others recognize specific isotopes within that species, like an anti-mouse IgG antibody. Some...subclass specific, like an anti-mouse IgG2a antibody. When using a monoclonal antibody be sure to use a highly...general anti-mouse IgG. In a broadly specific secondary antibody only a fraction of the antibody molecules...example, some goat anti-mouse secondary antibodies recognize some rat primary antibodies (Erickson 1993)....primary antibodies raised in different, and distantly related, species, and secondary antibodies raised...

  20. CRISPR-mediated Plant Base Editors

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    Blog Post
    ...editor would restore antibiotic resistance. To find such a protein, they created a mutant library of E. coli...improving base editing platforms for plant systems.  Figure 2: Plant base editor platforms and their...components. Adenine base editors for plant We developed plant adenine base editor (ABE) vectors to install... Cytosine base editors for plant There are several CBEs available for plant systems. Target-AID based ... Figure 3: Activity windows of plant base editors. Plant CBE and ABE with expanded PAM compatibility...constructed a number of plant base editors replacing the SpCas9 with the mutant versions of SpCas9 and ...the base editing scope in rice by using Cas9 variants." Plant biotechnology journal (2018). PubMed PMID:...
Showing: 41 - 60 of 1087 results