We narrowed to 76 results for: ARP
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TypeBlog Post...Hantman, A. W., Looger, L. L., Schaffer, D. V., & Karpova, A. Y. (2016). A Designer AAV Variant Permits Efficient...
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Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post... M., Gotthard, G., Tünnermann, J., Tänzer, T., Karpenko, J., Mertes, N., Xue, L., Uhrig, U., Reinstein... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...Prize included Jennifer Doudna and Emmanuelle Charpentier for their pioneering CRISPR/Cas9 work (now recognized... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects include... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog Post... focus). This means that the images will appear sharp without interference from blurry out-of-focus light... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...Fonfara I, Bouazza B, Schumacher CH, Bratovič M, Charpentier E, Möglich A (2013) Engineering of temperature... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post...mbio.00019-20 Singh AK, Carette X, Potluri L-P, Sharp JD, Xu R, Prisic S, Husson RN (2016) Investigating... -
Using AAV for Neuronal Tracing
TypeBlog Post...Hantman, A.W., Looger, L.L., Schaffer, D.V., and Karpova, A.Y. (2016). A designer AAV variant permits efficient... -
Gibson Assembly Protocol
TypeProtocol...one reaction. However, some labs have observed a sharp decrease in success rate when assembling more than... -
Trimmer Lab NeuroMab Collection
TypeCollection... Anti-TARPGamma2/Stargazin [N245/1R] TARPGamma2/Stargazin Rat Mouse IgG2a 177522 Anti-TARPGamma2/4/8 [... Mad3 Human Mouse IgG2a 220426 TARPGamma2/4/8 [N245/36R-2b] TARPGamma2/4/8 Rat Mouse IgG2a 220427 Kir2.3...IgG2a 231843 TARPGamma2/4/8 (Rattus norvegicus) recombinant mouse monoclonal antibody TARPGamma2/4/8 Rat Chimera... [N245/36R] TARPGamma2/4/8 Rat Mouse IgG2a 177523 Anti-Cav1.2 Ca2+ channel [N263/31R] Cav1.2 Ca2+ channel... -
CRISPR Library Amplification
TypeProtocol...Do so by gentle spreading. Some spreaders have a sharp edge that can scrape plates more abrasively at a... -
Retrograde AAV viral preps
TypeCollection... viral service. Retrograde construct from Alla Karpova at Janelia Research Institute....preparations in a retrograde serotype from the Alla Karpova and David Schaffer laboratories. These viral vector... these viral preparations was performed by the Karpova lab. Results for our quality control experiments... -
Neurodegeneration Plasmid Collection
TypeCollection...Parkinson's Wade Harper 22594 Flag-HA-OTUD4 OTUD4 HA, FLAG CMV Gordon Holmes Syndrome Wade Harper 23026 pET9d-hnRNP-A1...pHAGE-eGFP-OPTN OPTN ALS Wade Harper 175760 pHAGE-FLAG-APEX2-OPTN OPTN Flag CMV ALS Wade Harper 175762 pHAGE-eGFP-...GFP CMV ALS Wade Harper 175763 pHAGE-eGFP-OPTN S473A 513A OPTN GFP CMV ALS Wade Harper 175764 pHAGE-eGFP-OPTN...OPTN GFP CMV ALS Wade Harper 175765 pHAGE-eGFP-OPTN E50K OPTN GFP CMV ALS Wade Harper 176267 pAAV-FLEX-P301L...177094 pHAGE-APPSw;T719N APP CMV Alzheimer's Wade Harper 177511 Anti-Frataxin [N191/7R] FXN CMV Friedreich...Cookson 193772 pENTR221-APP751 APP Alzheimer's Wade Harper 194004 VPS13D(deltaATG2-C/PH)^EGFP VPS13D GFP CMV... -
Immunology Research Plasmids and Resources
TypeCollection...ARMET arginine-rich, mutated in early stage tumors ARP, MANF, MGC142148, MGC142150 ARMETL1 arginine-rich...ANG3, ANGPT3, ARP1, AngY, KIAA0351, UNQ162, dJ595C2.2 ANGPTL2 angiopoietin-like 2 ARP2, HARP, MGC8889 ANGPTL3...ANGPT5 ANGPTL4 angiopoietin-like 4 ANGPTL2, ARP4, FIAF, HFARP, NL2, PGAR, pp1158 ANGPTL5 angiopoietin-like...angiopoietin-like 5 - ANGPTL6 angiopoietin-like 6 AGF, ARP5 ANGPTL7 angiopoietin-like 7 AngX, CDT6, RP4-647M16.2, dJ647M16.1... nuclear receptor subfamily 2, group F, member 2 ARP1, COUP-TFII, COUPTFB, MGC117452, SVP40, TFCOUP2 NR2F6...HHM, MGC14611, PLP, PTHR, PTHRP PTN pleiotrophin HARP, HBGF8, HBNF, NEGF1 PYY peptide YY PYY1 QRFP pyroglutamylated... -
CRISPR History and Development for Genome Engineering
TypeCollection... Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. 2012. A programmable dual-RNA-guided DNA endonuclease...Shah SA, Saunders SJ, Barrangou R, Brouns SJ, Charpentier E, Haft DH, Horvath P, Moineau S, Mojica FJ, ...Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. 2015. In vivo genome editing using ... -
CRISPR Guide
TypeCollection...K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012). A programmable Dual-RNA–Guided DNA...Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, D. H., Horvath, P., Moineau, S., Mojica...Shalem, O., Wu, X., Makarova, K. S., Koonin, E. V., Sharp, P. A., & Zhang, F. (2015). In vivo genome editing...Biotechnology . 38 (7), 824–844. PMID: 32572269 Charpentier, M., Khedher, A. H. Y., Menoret, S., Brion, A... -
Zhang Lab CRISPR Page
TypeCollection...Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. Nature . 2015 Apr 9;520(7546):186-91...Langer 9, Anderson DG, Hacohen N, Regev A, Feng G, Sharp PA, Zhang F. Cell . 2014 Oct 9;159(2):440-55. doi... -
Antibody Guide
TypeCollection...weak signals due to their monovalent nature. Darpins - Darpins are genetically engineered small proteins ... -
All Antibodies
TypeCollection...other types of affinity reagents such as scFvs, DARPins, Fabs, and secondary antibodies. To learn more ... -
Recombinases AAV Preps
TypeCollection...produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory...