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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 304 results for: CO

Showing: 41 - 60 of 304 results

  1. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...for methanol usage. This gene promoter, AOX1, was co-opted for the expression of recombinant proteins ...Saccharomyces cerevisiae to human: The important gene co‑expression modules. Biomedical Reports 7.2 (2017)...

  2. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...trans-protein splicing. Note, however, that this will require co-delivery of two AAVs at a high MOI, often resulting...microglia in vivo.   Q5: What is the efficiency of co-delivering different AAV particles into the same ...

  4. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing the...first round of translation While ribosome profiling co-opts the physical location of the ribosome, TRICK...

  5. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ... rule, plasmids from the same group should not be co-transformed, so if you need two plasmids for an experiment...from the same incompatibility group should not be co-transformed. +Actual copy number varies. See below...

  7. CRISPR Plasmids - Yeast

    Type
    Collection
    ...etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  8. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...number of new platforms (such as openUC2, which I am a co-founder of), that aim to support the community in...the Heintzmann Lab at the Leibniz IPHT Jena. He is co-founder of the open-hardware startup “openUC2” and...

  9. Antibodies 101: Isotypes

    Type
    Blog Post
    ... with its variable chain domain. B cells start by co-expressing IgM and IgD antibodies, but later in an...recombination of the antibody gene locus. B cells start by co-expressing IgM and IgD isotypes antibodies which ...

  12. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...all organisms and cell types, (ii) no need to add a co-factor, (iii) easy visualization with standard filter...conditions, proteins that bind endogenous fluorescent co-factors can be used. Examples include the LOV domains...Kumagai, 2013). Next to these naturally occurring co-factor binding proteins, systems wherein a protein...

  13. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...and Women’s Hospital (BWH). Based on her experience co-directing Mentor Circle Programs at BWH and providing...is a Junior Faculty at Harvard Medical School/BWH, co-Director of the Mentor Circle Program at BWH, and...health strategy, and global health equity. She is also Co-founder/director of the non-profit foundation IMFAHE...

  14. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...trans-protein splicing. Note, however, that this will require co-delivery of two AAVs at a high MOI, often resulting...microglia in vivo.   Q5: What is the efficiency of co-delivering different AAV particles into the same ...

  15. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...for methanol usage. This gene promoter, AOX1, was co-opted for the expression of recombinant proteins ...Saccharomyces cerevisiae to human: The important gene co‑expression modules. Biomedical Reports 7.2 (2017)...

  17. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...Concentation*** Blasticidin bsd  HeLa, NIH3T3, CHO, COS-1, 293HEK Inhibits termination step of translation...termination 1-10 ug/mL Zeocin Sh bla HeLa, NIH3T3, CHO, COS-1, 293HEK, Jurkat T cells Complexes with DNA; causes...

  18. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...promoter sequence and transcription is ON. Once a co-repressor protein binds the repressor protein, the...promoter is less leaky than UAS in the basal state, and co-expression of the inducer QF can increase QUAS driven...human HeLa cells as compared to basal expression. Co-expression of repressor QS along with QF leads to...

  19. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...independently packaged into an AAV. When a cell is co-infected with both part A and part B AAVs, the fragments...of these variables influence whether cells will be co-transduced with multiple copies of both vectors. ...and “part B” of the transgene. These two AAVs are co-transduced in a single step and serve as donors for...

  20. Worm Expression Resources

    Type
    Collection
    ...Link opens in a new window) Seydoux lab website . Co-CRISPR plasmids that facilitate detection of genome-editing...Developed in Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis...
Showing: 41 - 60 of 304 results