Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 184 results for: CO

Showing: 41 - 60 of 184 results

  1. Rett Syndrome

    Type
    Collection
    ...ability to bind to methylated DNA and recruit known co-repressors or other transcriptional modifiers suggests...D omain (TRD) the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic acid and...

  2. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...HiBiT were added to LgBiT-expressing hepatocytes or co-cultured across very narrow filters, complementation... has been hampered by transient interactions with co-receptors (like NRP1) and by endocytic trafficking...sites and various isoforms led to subtle changes in co-receptor dynamics, including NanoBRET assays to monitor...

  3. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...independently packaged into an AAV. When a cell is co-infected with both part A and part B AAVs, the fragments...of these variables influence whether cells will be co-transduced with multiple copies of both vectors. ...and “part B” of the transgene. These two AAVs are co-transduced in a single step and serve as donors for...

  4. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...promoter sequence and transcription is ON. Once a co-repressor protein binds the repressor protein, the...promoter is less leaky than UAS in the basal state, and co-expression of the inducer QF can increase QUAS driven...human HeLa cells as compared to basal expression. Co-expression of repressor QS along with QF leads to...

  6. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...viruses to express genes or gene sets over 4.7 kb. Co-infection rates for AAVs are generally high enough...packaged in them and use elements such as IRES or 2A to co-express them from one promoter. Specificity The ...

  9. dTAG - You're it!

    Type
    Blog Post
    ...that bind an E3 ligase. These bifunctional degraders co-opt E3 ligases such as cereblon (CRBN) or von Hippel-Lindau...refer to as the degradation tag (dTAG) system, to co-opt the endogenous ubiquitin proteasome machinery...

  10. Addgene at SfN23!

    Type
    Blog Post
    ...workshop. Specifically, Jo, Brook, and Angela are co-leading "Making a Greater Impact Using Clear and ...

  11. Antibody Production

    Type
    Collection
    ... recombinant antibody preps contain low levels of co-purified plasmid from transfection. This residual...

  12. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    .... In this method, the major capsid protein VP1 is co-expressed with a fusion protein containing VP2C and... research, which required protein compartments to co-localize multiple enzymes in a reaction pathway, ...

  13. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... firefly luciferase activity in the Co-IP compared of a control Co-IP indicates stronger interaction. ...catalytic domain from DNA methyltransferase, DNMT3A. Co-expression of this modified Cas9 along with a gRNA... new method called DULIP (dual luminescence-based co-immunoprecipitation) to overcome the limitations ...Firefly luciferase. The bait and prey plasmids are co-transfected and proteins are immunoprecipitated from...tet-inducible OsTIR1 plasmid is also available). Second, they co-transfected short-arm donor vectors containing mAID..., and Cas9 activator expressing plasmids that can co-express 1-8 gRNAs. Ordon et al., 2016 tested the ...

  14. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6-pegRNA-GG-...

  15. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ... filaments with mGold2. Top: TIRF-SIM imaging of COS-7 cells expressing LifeAct fused to mVenus, mGold2s... plant model Arabidopsis (Weiss et al., 2025). By co-delivering TRV1 with TRV2 that encodes ISYmu1 and...

  19. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ... DBDs (see Figure 2a) (4). These fusions are then co-expressed in a yeast strain that also contains a ...your proteins are not post-translationally modified, co-expression of the enzyme responsible for the modification...

  20. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ... protein (e.g., GFP or luciferase) that is either co-transfected with the test plasmid or transfected ...how much plasmid DNA to use for any individual or co- transfection, should be determined empirically.  ...
Showing: 41 - 60 of 184 results