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We narrowed to 186 results for: Coli

Showing: 41 - 60 of 186 results

  1. Bacterial Expression Systems

    Type
    Collection
    ...Plasmids 101: E. coli strains for protein expression Plasmids 101: Common Lab E. coli Strains Why Bacteria...Bacteria? E. coli are commonly used to store and replicate plasmids of all types, but beyond that, researchers...researchers also use bacteria like E. coli and their relatively well understood biology to answer many interesting... an N-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease domain. pET-Sangamo-His...of a C-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease domain. Return...can be used to express a protein of interest in E. coli , B. subtilis and P. pastoris . All plasmids use...

  2. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ... of eight bacterial toxin genes isolated from E. coli or Psuedomonas aeruginosa. These toxins were a mix...Gram-negative Enterobacteriaceae including in E. coli O157:H7, Shigella flexneri, Enterobacter cloacae... et al. "Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated and tunable...simplified chromosomal gene replacement in Escherichia coli." Appl. Environ. Microbiol. 81.15 (2015): 5103-5114...Recombineering) system for genome editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed PMID...

  3. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...delight,” the very first protein they tested — E. coli expressing good ol’ EGFP — showed a slight but detectable...: Fluorescence intensity of EGFP-FlavinTag in E. coli changes in response to a ~25 mT magnet. Gray regions...magnetoresponse was clearly visible whether expressed in E. coli, in mammalian cells, or in purified form, and didn...license.   Video 1: Fluorescence movie of E. coli expressing MagLOV while a magnet is waved under ...

  4. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...into E. coli and plasmid assembly (re-circularization) occurs in vivo. We use XL-10 Gold E. coli, but have...plasmid in vitro, before transforming it into E. coli for propagation. However, while not widely known...successful recombination reported in all laboratory E. coli strains tested so far. The use of this pathway as...

  5. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...performed, the next step is to transform competent E. coli cells and select the positive clones. The entry ...expression clone. You will also need to use a E. coli strain sensitive to CcdB (e.g. DH5α, TOP10, Mach1...protein inhibits the growth of CcdB sensitive E. coli strains, most colonies should contain the desired...available for all kind of organisms from mammals to E. coli. Glossary of Gateway cloning vectors Vector type...

  6. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...remain the lovely whitish-cream color of standard E. coli. On the other hand, intact β-galactosidase produces...restriction digest. Make sure you use a proper E. coli strain (i.e. contains the lacZΔM15 mutation): XL1...the Addgene Blog Read Our Post on Common Lab E. coli Strains Learn to Use Yeast Two Hybrid Systems Browse...

  7. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...the late nineties, this was developed into an E. coli system, with the noncanonical amino acid in the ...expression of phosphoserine-including proteins in E. coli (Zhu et al., 2022). It uses the TAG codon for the...Phosphoserine into Recombinant Proteins in Escherichia coli. 12(21). https://doi.org/10.21769/BioProtoc.4541...

  8. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...elements required for plasmid maintenance within an E. coli cell, but vectors can be widely utilized across ...necessary components to allow shuttling between E. coli and yeast cells. To be useful in the lab, the vectors...Can this be used for auxotrophic selection in E. coli? Additional considerations HIS3 L-hisitidine no...

  9. CRISPR Library Amplification

    Type
    Protocol
    ...of CRISPR pooled plasmid libraries in Escherichia coli cells....amplification of a pooled-plasmid library in Escherichia coli cells. Pooled libraries contain tens to millions...Maxipreps rely on incremental, ordered cell lysis. E. coli cells are subject to lysis by freeze-thaw if not...

  10. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ... other organisms. If you’ve ever wondered why E. coli is safe to use in the lab but also causes outbreaks..., Gateway cloning relies on genes found on an E. coli fertility plasmid that also encodes a toxin, CcdB...to that toxin, CcdA. Knowing which strains of E. coli contain this F plasmid is critical for successful...Some bacteria possess plasmid-borne toxins like colicins and subtilisins to kill other bacteria in the ...plasmids carrying genes for bacterial toxins like colicin typically also carry the necessary resistance genes...survive their own secretions. Pro tip! Historically, colicin-producing plasmids have been referred to as “Col...

  12. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ... plasmid DNA extraction and purification from E. coli is the presence of the protein endonuclease I encoded...encoded by the gene endA. Nowadays, the most common E. coli strains used for plasmid amplification are endA ...

  13. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...library is transformed into E. coli for amplification. Transformed E. coli are allowed to grow on plates...is similar to amplifying a plasmid: transform E. coli with the pooled library DNA, allow the bacteria ...

  14. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...vectors are usually designed to shuttle between E. coli and Agrobacterium tumefaciens, a plant-infectious...species, for example, in both S. cerevisiae and E. coli. Auxotrophic selection is especially common in yeast...shuttling between. For example, if you are using E. coli simply for easy cloning and quick DNA production...

  16. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...chromatography. Purification of His fusion proteins from E. coli is generally more straightforward and of a higher...a Solubility Enhancer. In P. E. Vaillancourt, E. coli Gene Expression Protocols (Vol. 205, pp. 99–118)...more!   Resources and References   References Coligan, J. E., Dunn, B. M., Speicher, D. W., & Wingfield...

  17. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...transformed directly into chemically competent E. coli and transformants selected with the appropriate ...have been isolated from methylation competent E. coli for this procedure to work properly. Directly transform...

  18. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    .... Max Juchheim in this post. 3. Plasmids 101: E. coli Strains for Protein Production Addgenie Julian Taylor-Parker...explains why you might want to use a specialized E. coli strain for protein production and gives a quick ...

  19. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...acids E. coli and Mammalian Cells pEVOL-pAzF synthetase for p-azido-l-phenylalanine E. coli pEVOL-...proven most effective only in a few cell types (E. coli and Xenopus oocytes, mainly). Nevertheless, tRNA...pEVOL-pBpF synthetase for p-benzoyl-l-phenylalanine E. coli pAcBac1.tR4-MbPyl pyrrolysyl-tRNA synthetase Mammalian...
Showing: 41 - 60 of 186 results