We narrowed to 57 results for: FLP
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TypeGuide...have been developed, such as using the Cre-lox and Flp-FRT recombination systems to excise the packaging...
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Cre-lox system
TypeCollection...multiple tissues. FLP/FRT: Alternative recombinase system for the control of gene expression. Flp recombinase...orientation of the FRT sites. FlpO constructs Addgene Blog Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist...recombinase recognizes FRT sites. Flp binding to these sites can result in excision, inversion or translocation...129419 pCag FlpO-2A-Cre EV FlpO-2A-Cre CAG Mammalian Breunig 130986 pCag SE (Self-excising) FlpO-2A-Cre EV...pCAG-Cre Cre CAG Mammalian Chenn 26745 pDIRE iCre and FlpO, required for dual RMCE EF-1 alpha Mammalian Zeller... EV Self-excising FlpO-2A-Cre CAG Mammalian Breunig 131780 pCS2+PIF6CreN CreLite - Red light-inducible... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...developed three new versions. Flipbow employs the Flp recombinase/FRT system in place of Brainbow’s Cre... -
Fluorescent Protein Guide: Biosensors
TypeCollection...expression of GCaMP6m/s in cells depdent on Cre, Flp, or Vcre Comprehensive Dual- and Triple-Feature Intersectional...viral expression of sRGECO (constitutive or Cre- or Flp-dependent) Comprehensive Dual- and Triple-Feature... -
Brain Initiative Collection
TypeCollection...AAV1 pAAV-CAG-fDIO-mNeonGreen An AAV genome with Flp recombinase-dependent expression of mNeonGreen from...pAAV-EF1a-iFlpV can be used to generate AAV virus that will express light-inducible site-specific iFlpV recombinase... -
Site Directed Mutagenesis by PCR
TypeBlog Post...followed by Restriction Fragment Length Polymorphism (RFLP) analysis (right). Introduction of restriction site...particular site with fragment length polymorphism (RFLP) analysis. In this process, if your mutation introduces... duplication will evade detection in the initial RFLP analysis simply because the introduced restriction... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog Post...gene pXPR_068 Violet-excited GFP (vex) Rosa26-FlpO-ERT2; H11-Cas9 L-CHIME Knocks out a single...sequentially pXPR_220 Violet-excited GFP (vex) Rosa26-FlpO-ERT2; H11-Cas9 Table 1: X-CHIME systems Proof... -
Hot Plasmids - August 2020
TypeBlog Post...New serotypes for the Cre-dependent Flpo recombinase pEF1a-DIO-FLPo-WPRE-hGHpA, which is now also available... -
CRISPR/Cas9 FAQs Answered!
TypeBlog Post... doing Restriction Fragment Length Polymorphism (RFLP) (see Figure 4 of the Cong, et. al, 2013 Science... -
New Viral Vectors - Winter 2025
TypeBlog Post...Optogenetics Ofer Yizhar New serotype pAAV-syn-Flpo AAVrg Recombinase Ian Wickersham New viral prep ... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post...additional approach, plasmids such as pACBSR-hyg and pFLP-hyg will be used to create gene deletions of important... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection...Isocortex 230518 pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA AiP1211 AiE2051m FlpO Layer 6_IT_Car3 Isocortex 224000...Isocortex 222939 pAAV-DLX2.0-minBG-FlpO-WPRE-HGHpA AiP14532 DLX2.0 FlpO Pan GABAergic interneurons Isocortex...Isocortex 230607 pAAV-AiE2103m-minBG-FlpO-WPRE-HGHpA AiP1252 AiE2103m FlpO Lamp5 interneurons Isocortex 223971...subplate 230518 pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA AiP1211 AiE2051m FlpO Claustrum Cortical subplate 191720...214855 pAAV-AiE0779m_3xC2-minBG-FlpO-WPRE3-BGHpA AiP14201 AiE0779m_3xC2 FlpO DRD1 Medium spiny neurons (D1...Striatum 230871 pAAV-AiE0452h-minBG-FlpO-WPRE3-BGHpA AiP14573 AiE0452h FlpO DRD2 Medium spiny neurons (D2-MSN...3xSP10ins-AiE0354m-minRho-SYFP2-WPRE3-BGHpA AiP12040 AiE0354m FlpO Vip interneurons Isocortex 230101 pAAV-hsA2-AiE0219h-minRho-SYFP2... -
Caltech Systemic Capsids
TypeCollection... iDreV Zeng 140137 pAAV-EF1a-iFlpV EF1a light-inducible recombinase iFlpV Zeng 164450 CN1851-rAAV-hI56i-minBglobin-iCre... -
AAVED
TypeCollection...Breakfast and Welcome Melina Fan 9:30 AM Cre and Flpo Katherine Matho 10:15 AM AAV Retrograde Tracing ... -
Zhang Lab's CRISPR Frequently Asked Questions
TypeCollection... doing Restriction Fragment Length Polymorphism (RFLP) ( see Figure 4 of the Cong, et. al, 2013 Science... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection.... pyogenes Joung AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR 68347 Mammalian/AAV none S... -
Immunology Research Plasmids and Resources
TypeCollection...VEGFR2 KGFLP1 keratinocyte growth factor-like protein 1 MGC125746, MGC125747, MGC126891 KGFLP2 keratinocyte...