We narrowed to 442 results for: HAL;

...pCRISPR new spacer cloning pCRISPR PDF, 109 KB Mendenhall and Myers Mammalian: FLAG tagging endogenous ...tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF, 49 KB Vosshall and Matthews (Link opens in a new window) CRISPR...

...DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre...

...deep-tissue organs or whole animals presents unique challenges. Genetically-encoded fluorescent proteins most...

... cells. Other microbial opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins...

...PubMed (Link opens in a new window) . Dana, H., Chalbatani, G. M., Mahmoodzadeh, H., Karimloo, R., Rezaiean...

...Leible, D., Leung, K.S., Magno, C.A., Meeran, A., Michalska, J.M., Rieger, F., Wang, C., Wu, M., Church, G.M...

...by Microbe System Plasmids for Archaea Species Haloferax sp. Methanococcus maripaludis Methanothermobacter...

...early ageing-associated phenotypes. Tyner SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann ...

...GTPases and their GEFs and GAPs: concepts and challenges. Molecular Biology of the Cell , 30 (11), 1249...

...any application Balanced Salt Solution (BSS) An ophthalmic solution suitable for any application TMN200 ...

... 9 Joshua Sanes 209658 pAAV.CAGFLEX.(cyto).cpSFGFP.HaloTag CAG None Cre dependent 5 Tim Brown and HHMI-JRC...

...AAV collection, including channelrhodopsins, halorhodopsins, and more....

...Link opens in a new window) and was adapted from Challis et al. (2022). Text version of the AAV decision...

...iPSCs, much like their ES cell cousins, have the hallmarks of pluripotent cells--they are capable of differentiating...

... good tissue distribution, low toxicity, a known half-life (24 hours), and is relatively inexpensive. ...

...generally higher than HDR pegRNA optimization can be challenging Prime Edit Repress or Interfere (CRISPRi) Reduce...

...known as HaloTag is actually HaloTag7, and recent specialized variants run up to HaloTag11. HaloTag is now... (Gallagher et al., 2009). HaloTag: Scientists at Promega developed HaloTag in 2008 (Los et al., 2008)...2008), based on a bacterial haloalkane dehalogenase that binds a synthetic chloralkane (CA) ligand. The current...159 Trimpethoprim  (TMP-tag) Few Easy HaloTag 295 Chloralkane (CA) Many Easy CLIP-tag...proteins (often up to 10-fold more). So, labeling a HaloTag or SNAP-tag construct can help you better detect...) Labeling scheme. b) COS-7 cell ER labeled with Halo-Sec61β with SiR-CA (magenta) and outer mitochondrial...protein of interest. For example, the Cohen lab used HaloTag for their "ticker tape" method for intracellular...

...Cases present specific operational or commercial challenges or questions that clients face imminently. Consulting...on analyzing the situation that presented the challenge at hand and help resolve it through well-informed...this realm, there are a few different kinds of challenges that consultants get called upon to tackle, depending...to collaborate whenever it faces an important challenge. The types of projects mentioned above are just...rewarding parts of the job is being confronted with challenges that are crucial for the success of a drug’s ...reasons why client situations might be especially challenging. For instance, the client might need to get a...the next 3, 5, or 10 years. Complicating these challenges is the fact that they occur in the context of...

...Talk." 4. The chalk talk, simple in principle The first question I usually get about chalk talks is, what... whether there is chalk. Sometimes. It can also be a slide deck, markers, etc. Chalk or markers force ... Developing your Chalk Talk These items should be part of the beginning of your chalk talk. These should... This edition has substantial expansions on the chalk talks and Zoom interviews sections, along with new...what is a Chalk Talk? It is a 60-90 min session with department members to discuss your research plans...alternatives for an approach, if asked. The key to a chalk talk is to effectively convey your message. Key ...." This is only the first few minutes of the chalk talk. Realize that it is rare to make it through...

... making it all work – most of the time. #1 Thou shalt find enjoyment in your work If you are happy at ...Nobody wins, and you need to make a change. #2 Thou shalt sometimes put work ahead of life Sometimes, work...and with attention to those around you #4 Thou shalt not bear the burden alone You, alone, are not solely...absolutely equally, which means you only have one-half of the guilt. You can handle that, right? Big tip...get enmeshed in a serious relationship. #5 Thou shalt not sweat the small stuff The house can be messy...yourself; you will make progress over time. #6 Thou shalt throw money at the problem If you can, pay someone...to relax and be with friends and family. #7 Thou shalt create community to help Involve family. If you ...