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We narrowed to 237 results for: KIT

Showing: 41 - 60 of 237 results

  2. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...available in the Sybody Generation Toolbox plasmid kit: SB_concave, SB_loop, SB_convex. These scaffolds ... This selection step involves using a commercial kit for in-vitro translation of the mRNA libraries. Each...plasmids are available in the Sybody Generation Toolbox kit. Find the Seeger lab's SARS-CoV-2 sybodies! References...

  3. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ..., plasmid kits are avaliable for less than than it would cost to buy each plasmid in the kit individually...This describes how the plasmid or other product (kit, pooled library, etc) will be sent to you. Most of...

  4. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ... tools that you need. The Golden GATEway cloning kit simplifies the cloning process for complex DNA constructs...biological systems” as they predict. The pOSIP plasmid kit can be found at Addgene, so what are you waiting ...constructs, while the Patron Lab MoClo Plant Parts kit contains modules specific for plant transformation... For further reading about Nicola Patron's MoClo kit and her plant synbio research, read the Addgene interview...annealing (SSA) assay. This new Platinum Gate TALEN Kit utilizes a 4-module assembly system, which reduces...additional information on using the Platinum Gate TALEN Kit, please see the Yamamoto lab’s protocol for TALEN...specifically to improve plant transgenesis. TheGreenGate kit available at Addgene includes six individual types...

  5. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...and responsive voltage indicator ONE-GO biosensors kit Antibodies for neurodegeneration research   Optimized....1016/j.neuron.2024.08.019.  ONE-GO biosensors kit By Mike Lacy G protein-coupled receptors (GPCRs) ... of selectivity. With 10 plasmids available as a kit, plus 21 optional accessory plasmids, the collection...

  7. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...topoisomerase (2).  These days commercially available TOPO kits provide vectors or cloning arms with overhanging...covalently linked to topoisomerase. Vectors in these kits also often have the topoisomerase site inserted ...

  8. Plasmid Cloning by PCR

    Type
    Blog Post
    ...of the PCR reaction using a kit, such as the QIAquick PCR Purification Kit. The PCR product is now ready...

  9. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ... the entrepreneurial bug and has set up an ELISA kit company on whose website you can find some great ...great information on ELISA troubleshooting, ELISA kits and cell death assays. You can find Seán on twitter...

  10. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated AlcA promoter AlcR pGGA008...AlcA) and pGGC011 (AlcR) from red flame GreenGate kit Steroid-regulated LexA promoter XVE (synthetic...

  11. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...bacterial cultures. If you will be using a kit, follow the kit's instructions. If you want to perform plasmid...plasmid purification without using a kit, you can find a protocol for kit-free plasmid mini-prep at the bottom... , and (Link opens in a new window) Promega sell kits for isolating plasmid DNA in quantities as low as...concentration and purity click here . Top Protocol: Kit-free Alkaline Lysis Plasmid Miniprep Prepare the ...Store DNA at 4°C. Tips and FAQ Plasmid purification kits provide the fastest way to obtain a high concentration...improve the purity of plasmid DNA purified without a kit it is advisable to perform a phenol/chloroform extraction...This is included in the resuspension buffer of most kits. Reference Page Top Index...

  13. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...done, through a variety of methods or commercial kits.  However, antibodies conjugated to horseradish ...that can be activated to emit light with a simple kit, and is readily available on any number of secondary...

  14. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...little more colorful? CRISPRainbow is available as a kit from Addgene.   References 1. Chen, Baohui, et al...Fluorescent Protein Plasmids Find Fluorescent Protein Kits Find Empty Backbones with Fluorescent Protein Tags...

  15. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...Sandwich ELISA Most commercially available ELISA kits are for a sandwich ELISA. This technique works by...interested in detecting in your sample. When an ELISA kit is ordered, the wells are typically already coated...

  16. Antibodies 101: Conjugation

    Type
    Blog Post
    ...looking to conjugate antibodies yourself, there are kits available to make the process quick and easy. Before...antibody’s buffer is compatible with your conjugation kit, since many common antibody buffers and buffer additives...

  17. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ... can be done in-lab using commercially available kits or ordered as synthesis modifications when purchasing...choose from and you can opt to do-it-yourself with a kit or purchase it commercially synthesized with a label...

  18. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...unknown GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth’s lab also assembled...ligand-bound GPCRs of interest. The TRUPATH plasmid kit consists of 20 plasmids encoding 14 alpha, 2 beta...

  19. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...there are several commercially available p24 ELISA kits that can quantify p24 levels in a matter of hours...day, but tends to be less expensive than an ELISA kit. In this approach, viral RNA is first converted to...

  20. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...repository for DNA-based materials, including our popular kits and libraries. This DNA-based materials repository...
Showing: 41 - 60 of 237 results