...for instance, the challenge of attempting to simultaneously and selectively induce both cutting and regulation... targeting a different set of loci to induce simultaneous cutting and transcriptional activation or repression...

...Hoffman Voltage JEDI-2P voltage indicator for two-photon imaging Sustained deep-tissue voltage recording...Rafael Yuste Voltage Ultrafast fluorescent voltage sensor ASAP2f Subcellular Imaging of Voltage and Calcium...fluorescent voltage sensor. Nat Neurosci. 2014 Apr 22. Michael Lin Voltage Fluorescent voltage reporters...Campbell Voltage Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters... S0092-8674(22)00916-3. Francois St-Pierre Voltage Voltage Sensitive Fluorescent Protein (VSFP/Butterfly...fluorescent protein voltage probe. Neuron. 2012 Sep 6;75(5):779-85. Vincent Pieribone Voltage ArcLight-ST to...Jun 30;166(1):245-57. Michael Lin Voltage Ultrafast fluorescent voltage sensor for detection of single action...

...cytosine (C) into a uracil (U) base. Later the resultant U-G pair is either repaired by cellular mismatch...enables us to target more than one genomic site simultaneously.     These binary vectors could be used to ...

...of its ability to target multiple gene loci simultaneously, CRISPRa can also be used to map molecular ...nmeth.3871 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning...

...overnight revelation, rather it was the result of consultations between researchers and publishers. Nonetheless...encouraging authors to integrate datasets with their resultant figures. Instead of providing a “dead end” figure...

...channelrhodopsins and tools based on light-oxygen-voltage (LOV) domain. They can also be used in crosstalk-free...directed evolution approach applied to fluorescent voltage reporters. Nat Chem Biol 14, 352-360 (2018). Yu...

...gel purification. In gel purification, you use a voltage difference across a gel matrix (usually agarose...positioned toward the cathode (- charge). When the voltage is applied across the gel, the DNA migrates toward...

... tend to show their limits when it comes to simultaneously modifying multiple loci (multiplexing). Currently...(MAGE) (1). MAGE uses recombineering (2) to simultaneously incorporate multiple single-strand DNA (ssDNA...in a broad range of strains. Thus, pORTMAGE simultaneously allows genome editing and mutant library generation...

... With this technique, multiple genes can be simultaneously silenced if a consensus sequence is used (~... tissue for downstream characterization. To simultaneously address both limitations of the traditional...

...proteins. A type VII is already on the horizon (Altae-Tran et al., 2023)! References and Resources References...), 280–284. https://doi.org/10.1038/nature24049 Altae-Tran, H., Kannan, S., Suberski, A. J., Mears, K....

...pcDNA3-delta133HtrA-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14126 pET20b-delta133HtrA2 HTRA2 ...pET20b-delta133HtrA2 S142A HTRA2 His T7 Parkinson's L. Miguel Martins 16154 pET20b-delta133HtrA2 S142D ...pET20b-delta133HtrA2 S400A HTRA2 His T7 Parkinson's L. Miguel Martins 16156 pET20b-delta133HtrA2 S400D ...pET20b-delta133HtrA2 A141S HTRA2 His T7 Parkinson's L. Miguel Martins 16158 pET20b-delta133HtrA2 G399S ...Vogelstein 17229 pQE9-His-p97deltaD2 VCP His T5 ALS Hemmo Meyer 17230 pQE9-His-p97deltaD2(K251A) VCP His T5 ALS...Sudol 17800 pM-ErbB4-delta-kinase ERBB4 GAL4-BD ALS Marius Sudol 17801 pM-ErbB4-delta-kinase-PY1 mutant ...BFP-TDP43 deltaNTD] TARDBP CMV ALS Rajat Rohatgi 133325 pHBS1501 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaRRM] TARDBP...

...D, member 1 CD94 LGMN legumain AEP, LGMN1, PRSC1 LTA lymphotoxin alpha (TNF superfamily, member 1) LT,...sterile alpha motif containing 1 FLJ31641, RIFLE, TAL LTA lymphotoxin alpha (TNF superfamily, member 1) LT,...receptor delta constant - TRDD1 T cell receptor delta diversity 1 - TRDD2 T cell receptor delta diversity...receptor delta diversity 3 TCRD TRDJ1 T cell receptor delta joining 1 TCRD TRDJ2 T cell receptor delta joining...receptor delta joining 3 - TRDJ4 T cell receptor delta joining 4 - TRDV1 T cell receptor delta variable... IL1F5 interleukin 1 family, member 5 (delta) FIL1, FIL1(DELTA), FIL1D, IL1HY1, IL1L1, IL1RP3, MGC29840...

...carrying version 1 of the CellTag barcoding library. Version 1 of the Celltag library contains 19973 barcodes...carrying version 2 of the CellTag barcoding library. Version 2 of the Celltag library contains 4934 barcodes...carrying version 3 of the CellTag barcoding library. Version 3 of the Celltag library contains 5737 barcodes...Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version 1 Ready-to-use lentiviral... via direct fluorescence. Morris 115644 Morris CellTag Barcode Library Version 2 Ready-to-use lentiviral... via direct fluorescence. Morris 115645 Morris CellTag Barcode Library Version 3 Ready-to-use lentiviral...

...protein that enables imaging of two biosensors simultaneously   Researchers in the labs of Michael Lin ...mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring the activities of CaMKII and RhoA...

...production plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward Boyden...pGP-AAV-syn-FLEX-Volton2-ST-WPRE AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression...4AA-mNeon-ST A122D WPRE AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression...floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector...pAAV-EF1a-DIO-JEDI-2P-WPRE Double floxed genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector...2P-Kv-WPRE Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector...AAV9 pAAV-hSyn-JEDI-2P-WPRE Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector...

...73472 RabV CVS-N2c(deltaG)-mCherry-P2A-Cre Cre CMV RABV Jessell 73474 RabV CVS-N2c(deltaG)-Cre Cre CMV RABV... light-inducible Cre; C terminus Cre fused to PhyBdelta CMV Mammalian, Zebrafish Eisenhoffer 131782 pME-CreLite...pME-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in middle entry vector (Tol2 kit) Zebrafish...131785 pAAV-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in AAV donor/transfer vector Cbh...131786 pLenti-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector Cbh Lentiviral...to turn off the expression of one gene while simultaneously turning on another. The FLEx switch system ...

...Mouse IgG2a 114515 Anti-GABA(A)R, Delta [N151/3.3R] GABA(A)R, Delta Rat Mouse IgG2a 114516 Anti-Doc2b ...Mouse IgG2a 206657 Anti-GABA(A)R, Delta [N151/3R-2b] GABA(A)R, Delta Rat Mouse IgG2b 206658 Anti-Navbeta4...Mouse IgG2a 219470 Anti-Cavalpha2delta2 Ca2+ channel [N492/38R] Cavalpha2delta2 Ca2+ channel Mouse Mouse...Human Mouse IgG2a 220415 Cavalpha2delta2 Ca2+ channel [N492/39R] Cavalpha2delta2 Ca2+ channel Mouse Mouse...Mouse IgG2a 220416 Cavalpha2delta2 Ca2+ channel [N492/93R] Cavalpha2delta2 Ca2+ channel Mouse Mouse IgG2a...Human Mouse 206724 GABA(A)R, Delta scFv [N151/3] N151/3 scFv GABA(A)R, Delta Rat Mouse 206725 TRIP8b (constant...

... al. , 2010). Number of fragments assembled simultaneously Multiple fragments can be assembled in one ...

...Sensors GRAB_NE nLight Oxytocin (OT) Sensors GRAB_OT Voltage Sensors Archon Voltron JEDI-2P ATP Sensors iATPSnFR2...GRAB_OTmut (control) none Constitutive 1, 9 Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2...KGC-GFP-ER2] Syn Archon1 EGFP Cre dependent 8 Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST...ST-WPRE Syn Voltron2-ST none Cre dependent 1 GENIE Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-JEDI-2P-...

...the dye front looks like a “smile”, reduce the voltage. Turn off the power supply and unplug the electrophoresis...