Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 178 results for: Lta;

Showing: 41 - 60 of 178 results

  1. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...perform its helicase and nuclease functions simultaneously, by continuously unraveling dsDNA, pulling ...reeling of ssDNA and unwinding of dsDNA occur simultaneously, which creates a loop in the target strand ...

  2. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...cytosine (C) into a uracil (U) base. Later the resultant U-G pair is either repaired by cellular mismatch...enables us to target more than one genomic site simultaneously.     These binary vectors could be used to ...

  3. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...of its ability to target multiple gene loci simultaneously, CRISPRa can also be used to map molecular ...nmeth.3871 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning...

  5. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...channelrhodopsins and tools based on light-oxygen-voltage (LOV) domain. They can also be used in crosstalk-free...directed evolution approach applied to fluorescent voltage reporters. Nat Chem Biol 14, 352-360 (2018). Yu...

  6. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...gel purification. In gel purification, you use a voltage difference across a gel matrix (usually agarose...positioned toward the cathode (- charge). When the voltage is applied across the gel, the DNA migrates toward...

  7. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... tend to show their limits when it comes to simultaneously modifying multiple loci (multiplexing). Currently...(MAGE) (1). MAGE uses recombineering (2) to simultaneously incorporate multiple single-strand DNA (ssDNA...in a broad range of strains. Thus, pORTMAGE simultaneously allows genome editing and mutant library generation...

  9. Typing CRISPR Systems

    Type
    Blog Post
    ...proteins. A type VII is already on the horizon (Altae-Tran et al., 2023)! References and Resources References...), 280–284. https://doi.org/10.1038/nature24049 Altae-Tran, H., Kannan, S., Suberski, A. J., Mears, K....

  10. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...protein that enables imaging of two biosensors simultaneously   Researchers in the labs of Michael Lin ...mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring the activities of CaMKII and RhoA...

  11. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Hoffman Voltage JEDI-2P voltage indicator for two-photon imaging Sustained deep-tissue voltage recording...Rafael Yuste Voltage Ultrafast fluorescent voltage sensor ASAP2f Subcellular Imaging of Voltage and Calcium...fluorescent voltage sensor. Nat Neurosci. 2014 Apr 22. Michael Lin Voltage Fluorescent voltage reporters...Campbell Voltage Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters... S0092-8674(22)00916-3. Francois St-Pierre Voltage Voltage Sensitive Fluorescent Protein (VSFP/Butterfly...fluorescent protein voltage probe. Neuron. 2012 Sep 6;75(5):779-85. Vincent Pieribone Voltage ArcLight-ST to...Jun 30;166(1):245-57. Michael Lin Voltage Ultrafast fluorescent voltage sensor for detection of single action...

  12. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pcDNA3-delta133HtrA-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14126 pET20b-delta133HtrA2 HTRA2 ...pET20b-delta133HtrA2 S142A HTRA2 His T7 Parkinson's L. Miguel Martins 16154 pET20b-delta133HtrA2 S142D ...pET20b-delta133HtrA2 S400A HTRA2 His T7 Parkinson's L. Miguel Martins 16156 pET20b-delta133HtrA2 S400D ...pET20b-delta133HtrA2 A141S HTRA2 His T7 Parkinson's L. Miguel Martins 16158 pET20b-delta133HtrA2 G399S ...Vogelstein 17229 pQE9-His-p97deltaD2 VCP His T5 ALS Hemmo Meyer 17230 pQE9-His-p97deltaD2(K251A) VCP His T5 ALS...Sudol 17800 pM-ErbB4-delta-kinase ERBB4 GAL4-BD ALS Marius Sudol 17801 pM-ErbB4-delta-kinase-PY1 mutant ...BFP-TDP43 deltaNTD] TARDBP CMV ALS Rajat Rohatgi 133325 pHBS1501 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaRRM] TARDBP...

  13. Immunology Research Plasmids and Resources

    Type
    Collection
    ...D, member 1 CD94 LGMN legumain AEP, LGMN1, PRSC1 LTA lymphotoxin alpha (TNF superfamily, member 1) LT,...sterile alpha motif containing 1 FLJ31641, RIFLE, TAL LTA lymphotoxin alpha (TNF superfamily, member 1) LT,...receptor delta constant - TRDD1 T cell receptor delta diversity 1 - TRDD2 T cell receptor delta diversity...receptor delta diversity 3 TCRD TRDJ1 T cell receptor delta joining 1 TCRD TRDJ2 T cell receptor delta joining...receptor delta joining 3 - TRDJ4 T cell receptor delta joining 4 - TRDV1 T cell receptor delta variable... IL1F5 interleukin 1 family, member 5 (delta) FIL1, FIL1(DELTA), FIL1D, IL1HY1, IL1L1, IL1RP3, MGC29840...

  14. Lentiviral Prep Service

    Type
    Collection
    ...carrying version 1 of the CellTag barcoding library. Version 1 of the Celltag library contains 19973 barcodes...carrying version 2 of the CellTag barcoding library. Version 2 of the Celltag library contains 4934 barcodes...carrying version 3 of the CellTag barcoding library. Version 3 of the Celltag library contains 5737 barcodes...Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version 1 Ready-to-use lentiviral... via direct fluorescence. Morris 115644 Morris CellTag Barcode Library Version 2 Ready-to-use lentiviral... via direct fluorescence. Morris 115645 Morris CellTag Barcode Library Version 3 Ready-to-use lentiviral...

  15. Gibson Assembly Protocol

    Type
    Protocol
    ... al. , 2010). Number of fragments assembled simultaneously Multiple fragments can be assembled in one ...

  17. Western Blot

    Type
    Protocol
    ...out of the wells and into the gel. Increase the voltage to 150 V and continue running the gel until you...

  18. Brain Initiative Collection

    Type
    Collection
    ...production plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward Boyden...pGP-AAV-syn-FLEX-Volton2-ST-WPRE AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression...4AA-mNeon-ST A122D WPRE AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression...floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector...pAAV-EF1a-DIO-JEDI-2P-WPRE Double floxed genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector...2P-Kv-WPRE Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector...AAV9 pAAV-hSyn-JEDI-2P-WPRE Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector...

  19. Cre-lox system

    Type
    Collection
    ...73472 RabV CVS-N2c(deltaG)-mCherry-P2A-Cre Cre CMV RABV Jessell 73474 RabV CVS-N2c(deltaG)-Cre Cre CMV RABV... light-inducible Cre; C terminus Cre fused to PhyBdelta CMV Mammalian, Zebrafish Eisenhoffer 131782 pME-CreLite...pME-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in middle entry vector (Tol2 kit) Zebrafish...131785 pAAV-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in AAV donor/transfer vector Cbh...131786 pLenti-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector Cbh Lentiviral...to turn off the expression of one gene while simultaneously turning on another. The FLEx switch system ...
Showing: 41 - 60 of 178 results