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Addgene

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We narrowed to 59 results for: Neb

Showing: 41 - 59 of 59 results

  1. General Transfection

    Type
    Protocol
    ... an endonuclease negative E. coli strain such as NEB stable. Dilute 1:3 (µg DNA:µg PEI) in 500 µL total...

  2. Lentivirus Production

    Type
    Protocol
    ... an endonuclease negative E. coli strain such as NEB stable. Make a mixture of a total of 500 μL PEI-OptiPro...

  5. Molecular Biology Reference

    Type
    Guide
    ...information about restriction enzymes check out NEB's website . Insert The insert is the gene, promoter...(lac- proAB) [F traDelta36 proAB lacIqZDeltaM15] NEB Stable New England Biolabs F' proA+B+ lacIq ∆(lacZ...

  6. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...recombination-deficient bacteria strains, such as NEB Stable cells. These strains reduce the frequency ...

  7. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...Overview of NEBuilder HiFi DNA assembly method As with any well-executed cloning endeavor, NEBuilder requires...resource for planning an NEBuilder HiFi DNA Assembly mix reaction is the NEBuilder Assembly Tool which is...BioBrick® Assembly, Golden Gate Assembly, and NEBuilder® HiFi DNA Assembly. Gibson Assembly allows the...enzymes results in scarless assembly. Finally, the NEBuilder® HiFi DNA Assembly method from New England Biolabs...Plasmids 101 eBook! One of the features that makes NEBuilder HiFi so accurate (apart from the high-fidelity...PCR template. Some of the applications of the NEBuilder HiFi DNA Assembly Mix include:  Generation of...Generating constructs with multiple point mutations NEBuilder HiFi can simplify multi-position site-directed...

  8. Adenovirus Guide

    Type
    Guide
    ...recombination deficient bacterial strains, such as NEB Stable cells. These strains reduce the frequency ...

  9. Lentiviral Vector Guide

    Type
    Guide
    ...recombination-deficient bacteria strains, such as NEB Stable cells. These strains reduce the frequency ...

  11. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...selected for by manipulating the nebulization time and pressure. Nebulization units, which can be purchased...physical fragmentation strategy is preferable. Nebulization involves forcing DNA through a small opening...

  16. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...iTOP (induced transduction by osmocytosis and propanebetaine) to describe this method of protein transduction...
Showing: 41 - 59 of 59 results