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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 259 results for: OVA

Showing: 41 - 60 of 259 results

  1. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    Blog Post
    ...phosphopantetheinyltransferase) – These enzymes catalyze the covalent attachment of a fluorophore ligand to a peptide...ligands. New fluorophores As a corollary to these innovative labeling strategies, several groups—including...infusion of photons for your imaging experiment. Innovative labeling strategies and improved fluorophores...and manipulating proteins in live cells through covalent labeling." Nature chemical biology 11.12 (2015...

  2. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...Biotin is a cofactor that in its active form is covalently attached to the active site of key metabolic ...in cells and is the process by which biotin is covalently bonded to a specific lysine at the active site...the bond between them is one of the strongest noncovalent protein-ligand interactions found in nature. ...in live mammalian cells with biotin ligase and monovalent streptavidin.” Nature Protocols 3.3 (2008): 534...

  3. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...modification system and the scarless, Footprint-Free™ removal of cargo with the excision-only piggyBac™ transposase...stimulate homologous recombination and Footprint-Free™ removal of the selection cassette by excision-only piggyBac...site is left behind in the genome following donor removal, which alters the endogenous DNA sequence and has...nuclease thereby preventing re-cutting. Transposase removal of the cassette later restores the target sequence...

  4. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...reagents If you want to label intracellular proteins covalently with FRET acceptors, then biarsenicals are a ...The utility of these reagents is that binding is covalent, so free dye can be washed away. In addition, ...experimental design lends itself to creativity and innovation, valuable commodities in any NIH grant application...Stephen R. Adams, and Roger Y. Tsien. "Specific covalent labeling of recombinant protein molecules inside...

  5. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...did we get here in the first place? How CRISPR innovation affected software development CRISPR, like the...it, offers an interesting view into how rapid innovation affects the research community. I’ve likened ...That’s just 3 to 4 years ago. It is a bubble of innovation (a piñata), and software development has caught...future of CRISPR software is promising. Rapid innovation in this space has put us in a good position to...

  6. What's New in CRISPR - May 2019

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    Blog Post
    ...This time: E. coli genome-wide CRISPR inhibition Covalent tethering of DNA template to Cas9 SECURE base ...E. coli genome-wide CRISPR inhibition library Covalent tethering of DNA repair template to Cas9 Homology-directed...single-stranded oligodeoxynucleotide HDR template covalently attached to the Cas9-gRNA RNP complex via the...

  7. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ... wanted to be able to build assemblies through covalent bonds so that the assemblies wouldn’t fall apart...build a highly multivalent assembly connected by covalent bonds, so there were no weak links in the chain...2014 Mar 17. PubMed) Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial...

  8. Hot Plasmids - October 2022

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    Blog Post
    ...fungus-like protist Hyphochytrium catenoides (Govorunova et al. 2022). The authors show that HcKCR1 and...photoactivation of HcKCR1. Images adapted from Govorunova et al. 2022.     HcKCRs represent a long-...performance of HcKCRs. Find HcKCR plasmids here!   Govorunova EG, et al. Kalium channelrhodopsins are natural...

  9. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ... of bacterial genomes utilize this system (4). Makarova et al. grouped the systems into three distinct...programmable platform well-suited for various innovative biological and translative technologies. Addgene...1709–1712 (2007). CRISPRdb. Date: 2013-09-18. Makarova K, Haft DH, Barrangou R, Brouns SJJ, Charpentier...

  10. Meet the New Addgene Board Members!

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    Blog Post
    ...was the Head of Oncology Target Discovery at the Novartis Institutes for Biomedical Research. Drug development...are less common, they can be very impactful. At Novartis, Stegmeier deposited the ClonTracer Barcoding ...built create inefficiencies that ultimately make innovation and science slow,” Vandenberghe says. “The continuous...

  11. Plasmids 101: TOPO Cloning

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    Blog Post
    .... The energy from this breakage is stored as a covalent bond between the cleaved 3′ DNA strand and a tyrosyl...different DNA strand comes along, it can attack this covalent bond thus joining the two DNA strands and releasing...overhanging 3´ deoxythymidine (T) residues that are covalently linked to topoisomerase. Vectors in these kits...

  13. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...activity after ischemic brain injury Dr. Gohar Tsakanova, Institute of Molecular Biology of the NationalAcademy...Sciences of the Republic of Armenia, Armenia Gohar Tsakanova is a Senior Researcher who investigates the molecular...two-photon laser scanning microscope will allow Tsakanova to image cortical neurons and study changes in...

  14. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...She is particularly interested in exploring how innovative tools influence the direction of scientific research...    Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT Sloan School of Management...performance, and randomized control trials in innovation. Visit his website at: http://www.neil-t.com/...

  15. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...powerful tool in almost all disciplines. In biology, innovative new imaging approaches are constantly being developed...and disciplinary boundaries and can allow for innovative organizations and companies to provide problem-solving...s41467-020-19447-9 Hohlbein, J., Diederich, B., Marsikova, B. et al. Open microscopy in the life sciences...

  16. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...Perez-Pinera et al., 2013). In the years that followed, innovative methods greatly improved CRISPRa, expanding its...E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon...Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J,...

  17. CRISPR in the Clinic

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    Blog Post
    ...submissions to the UK, EU, and USA federal drug approval boards are being finalized, and it is very likely...limited to, ones targeting muscular dystrophy, cardiovascular disease, inflammatory diseases, blindness, ...primarily made in the liver. Similarly, for cardiovascular disease approaches targeting cholesterol, these...

  18. Illuminating Epigenetics with A FRET Based Biosensor

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    Blog Post
    ...that decreased their likelihood of death from cardiovascular disease but increased their risk of death from...paternal grandmothers early food supply influenced cardiovascular mortality of the female grandchildren." BMC...Gunnar, Lars O. Bygren, and Soren Edvinsson. "Cardiovascular and diabetes mortality determined by nutrition...

  19. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post
    ...//doi.org/10.1038/s41586-019-1323-z Peters JE, Makarova KS, Shmakov S, Koonin EV (2017) Recruitment of...Strecker J, Ladha A, Gardner Z, Schmid-Burgk JL, Makarova KS, Koonin EV, Zhang F (2019) RNA-guided DNA insertion...doi.org/10.1126/science.aax9181 Strecker J, Ladha A, Makarova KS, Koonin EV, Zhang F (2020) Response to Comment...

  20. Overcoming the AAV Size Limitation for CRISPR Delivery

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    Blog Post
    ...https://doi.org/10.1038/s41551-019-0501-5  Liu J-J, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo...S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b...
Showing: 41 - 60 of 259 results