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We narrowed to 532 results for: PAN

Showing: 41 - 60 of 532 results

  1. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu E, Gu W, Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020...standard laboratories. In the case of the COVID-19 pandemic, qPCR can be used to diagnose the presence of ...molecular and clinical diagnostic applications. The company Mammoth Biosciences has been started based on DETECTR...

  3. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...plasmids used in this publication at Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang...-cells were isolated from healthy individuals, expanded, and infected with HIV-1. For two HIV-1 strains...especially difficult given that the viral reservoir spans multiple organ systems. Both approaches may prove...

  5. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...Tsherniak A, Iorio F (2019) Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets. Nat... offers additional PAM options that can greatly expand gRNA options. Created with BioRender.com...exciting time for functional genomics, with an ever-expanding list of tools to probe gene function. The best...10.1038/s41587-020-0561-9 Dempster JM, Pacini C, Pantel S, Behan FM, Green T, Krill-Burger J, Beaver CM...

  6. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...DG, Thakore PI, Moreb EA, Rivera RM, Madhavan S, Pan X, Ran FA, Yan WX, Asokan A, Zhang F, Duan D, Gersbach... last, and more importantly, if it does extend lifespan. This work should be done in mouse and large animal...

  7. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...scale.     References Bezanilla M, Perroud P ‐F., Pan A, Klueh P, Quatrano RS (2005) An RNAi System in ... named Dicer (Figure 1). The plant kingdom has expanded its repertoire of Dicer enzymes and these different...conclusion was based upon numerous phenotype discrepancies between gene silencing and knockout methods ...

  8. CRISPR Guide

    Type
    Collection
    ...E., Chen, Y., Whitehead, E. H., Guimaraes, C., Panning, B., Ploegh, H. L., Bassik, M. C., Qi, L. S., Kampmann...., Strand, C., Joung, J. K., Kleinstiver, B. P., Pan, X., Huang, A., & Doench, J. G. (2020). Optimization...effects xCas9 3.7 - mutations in multiple domains to expand PAM recognition, increase specificity, and decrease...recognizes NNTA, a PAM sequence often found in plants, expanding the targeting range for plant genome engineering...differentiation. The system is reversible, with a companion CRISPRon to undo repression using a TET1-dCas9...cleave ssDNA non-specifically. Cas12a also has an expanded targeting range to AT-rich regions, as it recognizes.... (2018). Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science , 361 (6408), 1259–1262...

  9. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...to disease, including motor neurons (-3mnx1/Hb9), pan neuronal (elavl3/HuC), microglial/macrophage (mpeg1.1...).This toolbox adds new neuronal tools to the expanding gateway compatible vectors currently shared amongst...polycistronic cassettes and discovered reprogramming discrepancies, which they traced to a 9 amino acid N-terminal... did not lead to the observed reprogramming discrepancies. The polycistronic piggyBac transposon vectors...Podcast Segment    Photocleavable protein PhoCl expands optogenetics toolbox Increasingly popular Optogenetics...proteins (FP) for cellular imaging is constantly expanding. Case in point - check out the growing list of...new base editors with distinct PAM sequences, expanding the number of available target sites for base ...

  10. Antibody Guide

    Type
    Collection
    ...you select. Suggested panels for small multiplex experiments 4-color flow panel: BV421, FITC, PE, and ...fluorophore colors in flow cytometry, but the largest panels used currently are in the mid-forties. When designing...and APC 4-color microscopy panel: DAPI for nuclei, AF488, AF555, and AF647 Antibody Selection Selecting ...again. After filtering, narrow down further by company, price, and other factors like availability and...and shipping time. Choose antibodies from companies that are known to produce reliable antibodies. Be aware...antibody can and often is licensed to multiple companies for sale. Before purchasing, directly compare ...

  11. Plasmids for Stem Cell Research

    Type
    Collection
    ...reprogramming of adult pancreatic exocrine cells to beta-cells. Nature. 2008 Aug 27. Melton Pancreatic Acinar Cells.... Nature. 2011 Jun 29;475(7356):390-3. Suzuki Pancreatic Exocrine Cells Beta-cells Adenoviral Mouse In...Long-term persistence and development of induced pancreatic beta cells generated by lineage conversion of...factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells...

  12. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... 76809 PANK2 gRNA (BRDN0001149459) PANK2 hU6 Hallervorden-Spatz disease David Root 76810 PANK2 gRNA (BRDN0001162474...BRDN0001162474) PANK2 hU6 Hallervorden-Spatz disease David Root 76811 PANK2 gRNA (BRDN0001162362) PANK2 hU6 Hallervorden-Spatz...LRRK2 Parkinson's William Hahn 23583 pDONR223-PANK2 PANK2 Hallervorden-Spatz disease William Hahn 23620...Hallervorden-Spatz disease David Root 76812 PANK2 gRNA (BRDN0001144887) PANK2 hU6 Hallervorden-Spatz disease David...Ac5 Parkinson's Elisa Izaurralde 79516 5E26 (PANK2) PANK2 His, TEV polH Hallervorden-Spatz disease Cheryl...OPTN_Halo_C_allele OPTN Halo ALS Michael Ward 178145 PANK2_Halo_C_allele PANK2 Halo Hallervorden-Spatz disease Michael...

  13. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...blue, green, red, and ratiometric indicators An Expanded Palette of Genetically Encoded Ca2+ Indicators...Indicators to Image Dynamic Zn(2+) Secretion of Pancreatic Islets. Anal Chem. 2019 Oct 1;91(19):12212-12219...Molecules Target Description Article PI Acetyl-CoA PancACe acetyl-CoA sensor for bacterial or mammalian expression...Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging. PLoS One...(cGull) Generation of a cGMP Indicator with an Expanded Dynamic Range by Optimization of Amino Acid Linkers...encoded dopamine sensor YdLight1 and RdLight1 An expanded palette of dopamine sensors for multiplex imaging...

  14. p53 Pathway

    Type
    Collection
    ...The list of p53 transcriptional targets is ever-expanding; the best-characterized effects of p53 are in ... have been characterized as gain-of-function, expanding the mechanisms by which mutant p53 is known to...Oct;9(10):701-13. PubMed PMID: 19693097 . The expanding universe of p53 targets. Menendez D, Inga A, Resnick...

  15. COVID-19 Resources

    Type
    Collection
    ... global research community is moving quickly to expand the knowledge and understanding of COVID-19 and...Background The Coronavirus Disease-2019 (COVID-19) pandemic is caused by a novel virus strain from the Coronaviridae...Access Collections of COVID-19 related articles spanning multiple publishers can be found here: ASM COVID...

  16. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ... confluent, expand into larger vessels. A confluent well of a 6-well dish can be expanded into a 10 cm...reagent Day 3–14: Change media as needed Day 14–18: Expand and harvest stable cell lines Equipment Class II...undergo additional selection while the population expands. At this time, some researchers reduce the concentration...cm dish. A confluent 10 cm dish can be expanded into two 75 cm 2 flasks, etc. Pro-Tip This selection method...populations are growing well and have been sufficiently expanded, prepare cell stocks and/or harvest to test for...will have higher levels of expression. Consider expanding populations transduced with a variety of dilutions...Figure 1: Generation of monoclonal cell lines from expansion of individual A549 cells stably expressing Cas9...

  17. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ... Monitor cells for growth and expand cells Day 14–30: Analyze and expand monoclonal lines of interest ...cells have expanded but before they become over-confluent, trypsinize the cells and expand them to larger...After the monoclonal lines have been sufficiently expanded, screen the lines for transgene expression and...Figure 1: Generation of monoclonal cell lines from expansion of individual A549 cells stably expressing Cas9... in individual wells of a 96-well plate after expansion of single cells for 13 days. Note that growth ...Single cells from a stable cell pool were then expanded for an additional 3 weeks under blasticidin selection...stable cell pool (pool) and from individual cell expansions (monoclonal, 1-5). Wild-type A549 cells do not...

  18. Kit Free RNA Extraction

    Type
    Protocol
    ...). You can use either Isopropanol or Lithium Chloride for this step. Isopropanol (Option A): Add 1 volume...). You can use either Isopropanol or lithium Chloride for this step. Isopropanol (Option A): Add 1 volume...precipitation step, two options are also included: using Isopropanol or Lithium Chloride. This protocol was adapted...decontamination solution like RNase AWAY® or RNaseZAP® Isopropanol (for precipitation step, Option A) 7.5 M Lithium...volume of Isopropanol to the extracted aqueous layer. Incubate at -20°C for 1 hour. Lithium Chloride (Option...

  19. Immunology Research Plasmids and Resources

    Type
    Collection
    ...course of an infection, adaptive immune cells will expand and give rise to memory cells that can recognize...of a high avidity BCR through repeat cycles of expansion. Chemokines Chemokines, or chemotactic cytokines...of a high avidity BCR through repeat cycles of expansion. Symbol Name Synonyms AKT1 v-akt murine thymoma... beta B29, IGB CD81 CD81 molecule S5.7, TAPA1, TSPAN28 CHP calcium binding protein P22 SLC9A1BP CHP2 calcineurin...DEF3, HNP-3, HNP3, HP-3 DEFA5 defensin, alpha 5, Paneth cell-specific DEF5, HD-5, MGC129728 DEFB1 defensin...similarity 3, member B 2-21, C21orf11, C21orf76, ORF9, PANDER, PRED44 FAM3C family with sequence similarity 3...

  20. NETRF

    Type
    Collection
    ... and more effective treatments for carcinoid, pancreatic, and related neuroendocrine cancers. The NETRF...States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine Tumors...
Showing: 41 - 60 of 532 results