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We narrowed to 540 results for: PAN

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Showing: 41 - 60 of 540 results

  1. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...plasmids used in this publication at Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang...-cells were isolated from healthy individuals, expanded, and infected with HIV-1. For two HIV-1 strains...especially difficult given that the viral reservoir spans multiple organ systems. Both approaches may prove...

  2. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...125.27 (2003): 8074–8075. PubMed PMID:12837056. 8. Pan, J. feng, Liu, N. hua, Shu, L. yuan, & Sun, H. “Application...

  4. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu E, Gu W, Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020...part of the world manifesting as a full-fledged pandemic. Containing the spread of this virus has become...widespread testing is crucial in controlling this pandemic. Currently, the CDC uses RT-qPCR tests to diagnose...

  5. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu E, Gu W, Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020...standard laboratories. In the case of the COVID-19 pandemic, qPCR can be used to diagnose the presence of ...molecular and clinical diagnostic applications. The company Mammoth Biosciences has been started based on DETECTR...

  7. CRISPR Guide

    Type
    Collection
    ...E., Chen, Y., Whitehead, E. H., Guimaraes, C., Panning, B., Ploegh, H. L., Bassik, M. C., Qi, L. S., Kampmann...., Strand, C., Joung, J. K., Kleinstiver, B. P., Pan, X., Huang, A., & Doench, J. G. (2020). Optimization...effects xCas9 3.7 — mutations in multiple domains to expand PAM recognition, increase specificity, and decrease...recognizes NNTA, a PAM sequence often found in plants, expanding the targeting range for plant genome engineering...differentiation. The system is reversible, with a companion CRISPRon to undo repression using a TET1-dCas9...cleave ssDNA non-specifically. Cas12a also has an expanded targeting range to AT-rich regions, as it recognizes.... (2018). Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science , 361 (6408), 1259–1262...

  8. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...DG, Thakore PI, Moreb EA, Rivera RM, Madhavan S, Pan X, Ran FA, Yan WX, Asokan A, Zhang F, Duan D, Gersbach... last, and more importantly, if it does extend lifespan. This work should be done in mouse and large animal...

  9. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...scale.     References Bezanilla M, Perroud P ‐F., Pan A, Klueh P, Quatrano RS (2005) An RNAi System in ... named Dicer (Figure 1). The plant kingdom has expanded its repertoire of Dicer enzymes and these different...conclusion was based upon numerous phenotype discrepancies between gene silencing and knockout methods ...

  10. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...Tsherniak A, Iorio F (2019) Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets. Nat... offers additional PAM options that can greatly expand gRNA options. Created with BioRender.com...exciting time for functional genomics, with an ever-expanding list of tools to probe gene function. The best...10.1038/s41587-020-0561-9 Dempster JM, Pacini C, Pantel S, Behan FM, Green T, Krill-Burger J, Beaver CM...

  11. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...to disease, including motor neurons (-3mnx1/Hb9), pan neuronal (elavl3/HuC), microglial/macrophage (mpeg1.1...).This toolbox adds new neuronal tools to the expanding gateway compatible vectors currently shared amongst...polycistronic cassettes and discovered reprogramming discrepancies, which they traced to a 9 amino acid N-terminal... did not lead to the observed reprogramming discrepancies. The polycistronic piggyBac transposon vectors...Podcast Segment    Photocleavable protein PhoCl expands optogenetics toolbox Increasingly popular Optogenetics...proteins (FP) for cellular imaging is constantly expanding. Case in point - check out the growing list of...new base editors with distinct PAM sequences, expanding the number of available target sites for base ...

  12. Antibody Guide

    Type
    Collection
    ...you select. Suggested panels for small multiplex experiments 4-color flow panel: BV421, FITC, PE, and ...fluorophore colors in flow cytometry, but the largest panels used currently are in the mid-forties. When designing...and APC 4-color microscopy panel: DAPI for nuclei, AF488, AF555, and AF647 Antibody Selection Selecting ...again. After filtering, narrow down further by company, price, and other factors like availability and...and shipping time. Choose antibodies from companies that are known to produce reliable antibodies. Be aware...antibody can and often is licensed to multiple companies for sale. Before purchasing, directly compare ...

  13. Plasmids for Stem Cell Research

    Type
    Collection
    ...reprogramming of adult pancreatic exocrine cells to beta-cells. Nature. 2008 Aug 27. Melton Pancreatic Acinar Cells.... Nature. 2011 Jun 29;475(7356):390-3. Suzuki Pancreatic Exocrine Cells Beta-cells Adenoviral Mouse In...Long-term persistence and development of induced pancreatic beta cells generated by lineage conversion of...factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells...

  14. Resolute Plasmid Collection

    Type
    Collection
    ... research in small biotech and pharmaceutical companies. The RESOLUTE project has received funding from..., I., Onstein, S., Klimek, C., Barbosa, B., Pantielieieva, A., Dvorak, V., Hannich, T. J., Schoenbett,... V., Steck, A. L., Sundström, H., Wilhelm, M., Steppan, C. M. (2020) The RESOLUTE consortium: unlocking... Oliveira, T., Penninger, J. M., Sauer, D. B., Steppan, C. M., Viollet, C., Klavins, K., Hannich, J. T...

  15. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...protein function and signaling networks continues to expand. The Rinehart and Söll labs at Yale University ...Noren, C. J., Rinehart, J., & Söll, D. (2011). Expanding the Genetic Code of Escherichia coli with Phosphoserine...Additional Resources See our dedicated Genetic Code Expansion collection page Read about Using Phosphoserine...Phosphorylation Read about GCE4All: Making Genetic Code Expansion Accessible...

  16. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ..., including green, blue, and red indicators An Expanded Palette of Genetically Encoded Ca 2+ Indicators...Indicators to Image Dynamic Zn 2+ Secretion of Pancreatic Islets. Anal Chem. 2019 Oct 1;91(19):12212-12219...Molecules Target Description Article PI Acetyl-CoA PancACe acetyl-CoA sensor for bacterial or mammalian expression...Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging. PLoS One...(cGull) Generation of a cGMP Indicator with an Expanded Dynamic Range by Optimization of Amino Acid Linkers...encoded dopamine sensor YdLight1 and RdLight1 An expanded palette of dopamine sensors for multiplex imaging...

  17. E11 Bio PRISM Collection

    Type
    Collection
    ...PRISM protein tags have been validated for use in expansion microscopy, allowing for super-resolution light... al., 2025). These reagents have been used in expansion microscopy workflows to achieve high-resolution...protein bits. B) Multiplex imaging of barcodes with expansion microscopy and reconstruction of neurons with ...

  18. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... 76809 PANK2 gRNA (BRDN0001149459) PANK2 hU6 Hallervorden-Spatz disease David Root 76810 PANK2 gRNA (BRDN0001162474...BRDN0001162474) PANK2 hU6 Hallervorden-Spatz disease David Root 76811 PANK2 gRNA (BRDN0001162362) PANK2 hU6 Hallervorden-Spatz...LRRK2 Parkinson's William Hahn 23583 pDONR223-PANK2 PANK2 Hallervorden-Spatz disease William Hahn 23620...Hallervorden-Spatz disease David Root 76812 PANK2 gRNA (BRDN0001144887) PANK2 hU6 Hallervorden-Spatz disease David...Ac5 Parkinson's Elisa Izaurralde 79516 5E26 (PANK2) PANK2 His, TEV polH Hallervorden-Spatz disease Cheryl...OPTN_Halo_C_allele OPTN Halo ALS Michael Ward 178145 PANK2_Halo_C_allele PANK2 Halo Hallervorden-Spatz disease Michael...

  19. p53 Pathway

    Type
    Collection
    ...The list of p53 transcriptional targets is ever-expanding; the best-characterized effects of p53 are in ... have been characterized as gain-of-function, expanding the mechanisms by which mutant p53 is known to...Oct;9(10):701-13. PubMed PMID: 19693097 . The expanding universe of p53 targets. Menendez D, Inga A, Resnick...

  20. Immunology Research Plasmids and Resources

    Type
    Collection
    ...course of an infection, adaptive immune cells will expand and give rise to memory cells that can recognize...of a high avidity BCR through repeat cycles of expansion. Chemokines Chemokines, or chemotactic cytokines...of a high avidity BCR through repeat cycles of expansion. Symbol Name Synonyms AKT1 v-akt murine thymoma... beta B29, IGB CD81 CD81 molecule S5.7, TAPA1, TSPAN28 CHP calcium binding protein P22 SLC9A1BP CHP2 calcineurin...DEF3, HNP-3, HNP3, HP-3 DEFA5 defensin, alpha 5, Paneth cell-specific DEF5, HD-5, MGC129728 DEFB1 defensin...similarity 3, member B 2-21, C21orf11, C21orf76, ORF9, PANDER, PRED44 FAM3C family with sequence similarity 3...
Showing: 41 - 60 of 540 results