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We narrowed to 232 results for: SPL;

Showing: 41 - 60 of 232 results

  1. Pooled Library Amplifications

    Type
    Blog Post
    ...from the Mark Dawson lab Re-transformation of the SPLINTR Libraries Protocol.   Selecting your strain...perform several individual transformations, such as splitting 400 ng of DNA in 100 μL of cells over four individual...Electroporation Protocol (DOCX, 15.8 KB) Barcode SPLINTR Pooled Libraries 200,000–1,200,000 60 bp ... 200 ng Liquid Re-transformation of the SPLINTR Libraries (PDF, 401 KB) Screening Zhang Lab...

  2. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...can be found at Addgene. Split FPs – some FPs (e.g. GFP, Venus) can be split into two halves, which are...proximity, they will form the full FP and fluoresce. Split FPs can be used to determine the proximity of two...two proteins fused to the halves of the split FP. This technique is also is also called Bimolecular Fluorescence...

  3. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...fusion proteins for imaging, tracking, modulating splicing, and regulating expression of specifically targeted...described in bacterial cells, LwaCas13a does not display nonspecific RNA cleavage activity in eukaryotic... LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency but no significant...to see this group of enzymes continue to make a splash in the CRISPR field. We feel that the culture of...

  4. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ... of interest do not lie beyond the dimensions displayed in the plot. (B) A more restrictive gate results... second measurement to choose for graphically displaying your events. You are free to choose any size-... the positioning and shape of the populations displayed. As the separation pattern largely stays the same... cells but not dendritic cells. Using a graph displaying both stains, I can quickly separate T killer ...

  5. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...antibodies (rAbs) either produced de novo using yeast display technology or produced from hybridoma antibodies...The 11 IPI synthetic rAbs, developed using yeast display technology, are directed to integrins that bind...fragments (Fabs) that bound an amino acid region displayed by a subset of the RGD-binding integrins containing...Synthetic integrin antibodies discovered by yeast display reveal αV subunit pairing preferences with β subunits...

  6. Rett Syndrome

    Type
    Collection
    ...RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the impacts...2002. Insight into Rett syndrome: MeCP2 levels display tissue- and cell-specific differences and correlate...

  7. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...because the top figure shows the construct from our 'Split RNA' design, where the pre-processing form of crRNA... more details. One important note is that this ‘split RNA’ design is not as efficient in genome editing...

  8. Plan Your Experiment

    Type
    Collection
    ...should be made HDR requires a repair template and displays reduced efficiency compared to NHEJ knockout. ...region is removed from the mRNA due to alternative splicing. Exons near the N-terminus are targeted since ...

  9. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...proteins (PA-FPs) are fluorescent proteins that display unique changes in their spectral properties upon...population that is more neutral, causing PA-GFP to display almost no fluorescence when illuminated with 488nm...fact, many of the early reversible PA-FPs also display this “negative” photoswitching. In 2008, scientists...than mEos2 (mEos4b is completely monomeric) and display bright and photostable green and red states. Beyond...

  10. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Bacterial vector for Myc tag pETcon(-) - Yeast surface display vector with a C-terminal Myc tag pPMW-attB - pUASp...expression (Gateway) pYD1 - Expression, secretion, and display of proteins on the extracellular surface of S. ...

  11. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ... a wide audience. The dashboard that I made to display the data in the CSV file is open for anyone to ...Addgene Requests Dashboard   The dashboard will display the following items (Fig. 1): A row with four... the CSV is shown (figure 2) and the dashboard displays the data in a standardized way. Since Addgene ...

  12. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...potential strategy is to use split-inteins to mediate trans-protein splicing. Note, however, that this will...scenario, you may be best served by a dual vector, split intein approach.   Q3: What’s the best method for...

  15. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...(AAV). The components of these vectors are also split across several plasmids (often three), for safety...virus must assemble and have appropriate factors displayed on the outside of the particle to interact with...components needed for viral packaging, assembly, and display. Pol, the last component, contains the reverse ...

  18. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...talking to themselves as they compare the features displayed on the model with their mental model of the protein...primed for cleavage. This structure captures the displaced non-target DNA strand and shows the HNH nuclease...target DNA strand. In addition, this model also displays the several Lys/Ala mutations reported by Ian ...

  19. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...each sample. Successfully analyzed samples will display the following parameters: Sample - The unique ...analysis can be sorted by any of the parameters displayed in the summary table. You can also use your browser..., you’ll find an Indel plot (left panel) which displays the inferred distribution of indel sizes in the...

  20. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...sequence NGG compared to wild-type SpCas9, which also displays some cleavage with an NGA PAM. This variant may... AAV delivery. NmCas9, another small ortholog, displays lower off-target editing than wild-type SpCas9...rather than the blunt ends created by Cas9. Cas12a  displays lower off-target editing than SpCas9 (Kim et al...
Showing: 41 - 60 of 232 results