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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 728 results for: STO;

Showing: 41 - 60 of 728 results

  1. Antibody Production

    Type
    Collection
    ...is compared to an untransfected control. Immunohistochemistry (IHC) Tissue sections are fixed, permeabilized...Neuromab Protocols (Link opens in a new window) page. Storage and Shipping Stability Addgene does not recommend... suggests preparing single-use aliquots before storing at -20 °C. Each antibody catalog item is tested...some of our other resources to help you choose, store, or use your antibody. Antibody Blog Posts Recipient...

  2. Fluorescent Proteins: FRET

    Type
    Collection
    ...calculator (Link opens in a new window) *: Long Stokes shift donor **: Non-fluorescent (dark) acceptor...Protocol for deriving proximity, affinity, and stoichiometry of protein interactions using image-based quantitative...backbones for various FRET pairs with mTurquoise2 Mastop et al. (2017) Characterization of a spectrally ...biosensors in Addgene's Biosensors Collection . Generate custom FRET-based biosensors with the cpFRET kit from ...

  3. Luciferase Plasmid Collection

    Type
    Collection
    ...screening of transformants in cloning process. Lynne Postovit 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter...allowing the study of biscistronic promoters. Daniel Christophe 119760 pSGDlucV3.0 Firefly, Renilla Dual reporter... Lentiviral expression of firefly luciferase Christopher Vakoc 33307 Ubc.Luc.IRES.Puro Firefly Ubc Lentiviral...is expressed if cells are infected with virus Christopher Buck 98580 pLX304 Luciferase-V5 blast Firefly...

  4. AAV Viral Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Browse In-Stock AAV Adeno-associated virus (AAV) vectors are safe...protection levels. High quality and high titer in-stock AAV preps are affordable, available in small volumes... testing, and viral genome sequencing (VGS). In-stock AAV preps are distributed as 100 µL solutions of...

  5. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ... for any additional customs fees or taxes, and to complete any additional customs documentation that might...research materials. Please be aware that additional customs charges may be required before your order will ...

  6. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...References Rapid 'open-source' engineering of customized zinc-finger nucleases for highly efficient gene... (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML et al. Nat Protoc...19798082 . Targeted mutagenesis in zebrafish using customized zinc-finger nucleases. Foley JE et al. Nat Protoc...

  7. Genetic Code Expansion

    Type
    Collection
    ...challenging. In E.coli , the rarest codon is the amber stop codon (UAG) and thus this codon is often used. The...sites changes to UAG, RF1 function removed, MutS restored so decreased mutation rate George Church " 68306...Ochre (rEcΔ2.ΔA.B3.tW*) Utilizes UAA as the sole stop codon, with UGG encoding tryptophan and UAG and ... Derived from C321.deltaA, by replacing 1195 TGA stop codons with the synonymous TAA, and engineering ...

  8. Neurodegeneration Research Collection

    Type
    Collection
    ...most neurodegenerative diseases are only partly understood, and there are no effective therapies to prevent...symptom onset. Disease mechanisms are still poorly understood but several genes and mapped loci seem to be ...95%) of ALS are sporadic, having no prior family history. A small percentage (5-10%) are familial ALS cases...

  9. Rett Syndrome

    Type
    Collection
    ...almost 46% of all Rett syndrome cases. Research Milestones 1992 - MECP2 is discovered by Adrian Bird's lab...regardless of age or symptom severity, suggesting that restoring MECP2 expression in humans could be curative. ...therapeutic strategies to treat Rett syndrome and restore functional MECP2 in cells. Search (Link opens in...

  10. Optogenetics AAV Preps

    Type
    Collection
    ....1 CaMKII C1V1 (t/t) mScarlet Constitutive 9 Christopher Harvey 59170 pAAV-Syn-Chronos-GFP Syn Chronos...ChrimsonR (soma-targeted) mRuby2 Cre dependent 9 Christopher Harvey 108912 pAAV-CAG-DIO-ChroME-ST-P2A-H2B-...ChrimsonR (soma-targeted) mScarlet Constitutive 1, 9 Christopher Harvey 130991 pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1...

  11. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...protein purification. Just remember to remove the stop codon for C-terminal tags and omit the start codon... pDest vector that adds an N-terminal myristoylation signal GST Protein purification...targeting TALEN kits - Construct a custom TALEN array for genomic engineering Luciferase ...

  12. Plasmids for Stem Cell Research

    Type
    Collection
    ...reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming...from four separate plasmids Defining molecular cornerstones during fibroblast to iPS cell reprogramming ...Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell. ...

  13. Tetracycline Inducible Expression

    Type
    Collection
    ...dox-regulated plasmids . Looking for viral preps? Find in-stock viral preps of tetracycline transactivators and ...Rheenen 104102–104112 TetBow Series of plasmids for stochastic multicolor labeling for discriminating between...state of each cell in a population. Proliferation history is monitored by the dilution of a dox-inducible...

  14. Plan Your Experiment

    Type
    Collection
    ...using CRISPR . Browse Addgene's collection of in-stock lentiviral preps . AAV vectors AAV vectors offer...overcoming the AAV size limitation . Browse Addgene's in-stock AAV preps or try Addgene's Packaged on Request service...paired with restriction enzymes for further customization Usually paired with a sequencing method Can’...

  15. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Green Yellow Orange Red Far-Red Near-Infrared Long Stokes Shift Photoactivatable Photoconvertible Photoswitchable...pAAV-CAG-miRFP720 - Mammalian AAV Expression Return to top Long Stokes Shift These proteins have a large gap between their... their excitation and emission maxima (Stokes shift), useful for applications like multicolor imaging ...

  16. Antibodies

    Type
    Collection
    ...summarize antibody production and storage techniques, and explain common applications. View...some of our other resources to help you choose, store, or use your antibody. Antibody Blog Posts Recipient...

  17. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...Many SGC plasmids from the Toronto, Oxford and Stockholm sites have been deposited at Addgene and are available...Collection By All Plasmids SGC Toronto SGC Oxford SGC Stockholm Bacterial Expression Plasmids (empty backbones...

  18. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...purification, and comprehensive quality control. In-Stock Preps 1,000+ preps ready to ship immediately after... viral vector from Addgene, with details about storage. Biosafety Guide Documentation for your institutional...

  19. All Antibodies

    Type
    Collection
    ...species. We currently assess western blot, immunohistochemistry, and immunocytochemistry. Addgene will continue...some of our other resources to help you choose, store, or use your antibody. Antibody Blog Posts Recipient...
Showing: 41 - 60 of 728 results