We narrowed to 365 results for: Scrib

... Rather than being transcribed from different promoters, the gRNAs are transcribed together and are flanked...like AAV. In addition to the mammalian option described below, plasmids for making polycistronic gRNAs...oligonucleotide assembly.  Unlike some of the plasmids described above, this vector does not contain Cas9, so you... are similar to the Csy4 polycistronic system described above, except that they use an endogenous nuclease...

...database describing nearly 145,000 cell lines. Cellosaurus Cellosaurus seeks to identify and describe all ...authors of the paper in which the cell line was described. Using Cellosaurus Cellosaurus is searchable by...Cellosaurus received a report that HT-55, which was described as a colon cancer cell line, is actually a rectal...

...fly fishing, and local grizzly bear attacks. He described one such recent attack as "hilarious". Welcome... Mehmet Fatih Bolukbasi from Scot Wolfe's lab described a chimeric Cas9 that is improving the nuclease...also got to show off our new CRISPR page (which describes how to plan your CRISPR experiment for our new...next week, so follow us on Facebook, Twitter, or subscribe to this blog. Find more CRISPR and Genome Engineering...

...for CRISPRi are described here: http://www.addgene.org/crispr/qi/ Plasmids described in  Zalatan et ...www.addgene.org/browse/article/9593/ Plasmids described in Qi et al. Cell  (2013) can be found here: https...Aptamer base: a collaborative knowledge base to describe aptamers and SELEX experiments. Database, bas006...

... to our website through the use of alt text to describe images in online spaces. Alt text is used by screen...example. Remember, our goal is to use words to describe the image in a way that helps visually impaired... your alt text, use all of this information to describe the image helpfully. You and your readers are ...write alt text for any image you’re struggling to describe in the results. And many journals accept alt text...

...Requirement and Expanding CRISPR Beyond SpCas9 described the PAM specificity of Cas9s isolated from different..., and NGCG). Since then, the Osamu Nureki Lab described a rationally engineered Cas9, SpCas9-NG, which...Nishimasu et al., 2018). The David Liu Lab similarly described xCas9, which displayed the ability to target multiple...post on choosing a Cas9, the Feng Zhang Lab had described eSpCas9, while the Keith Joung Lab had developed...

...immune response. Over 60 interleukins have been described. NK Cell Signaling Natural killer (NK) cells can...immune response. Over 60 interleukins have been described. Symbol Name Synonyms EBI3 Epstein-Barr Virus ...

...both prokaryotic and eukaryotic systems must be transcribed into RNA, which occurs in three phases: initiation...Terminators are found downstream of the gene to be transcribed, and typically occur directly after any 3’ regulatory...Polymerases I, II, and III), each responsible for transcribing different types of RNA: Polymerase I is responsible...responsible for mRNA and miRNAs, and Polymerase III transcribes tRNA and other short RNAs. Although not as well...

...how will it be sent to me? Description: This describes how the plasmid or other product (kit, pooled ...vector’s plasmid number here. Gene/insert: This describes what has been inserted into the backbone, and ...they’ll end up on the N or C terminus of the transcribed and translated insert. If it is an empty vector... sequencing. Growth in Bacteria: This section describes how to grow the bacterial strain in which the ...

...worked extensively with enChIP systems. Can you describe this technology and its advantages? Fujii: In ...enChIP system using CRISPR technology. Can you describe this technology and some of its potential applications... any other technologies that you would like to describe?  Fujii: I believe that "Biology is Method", i.e...Hodaka Fujii lab's Addgene page and click the "Subscribe to Alerts" link in the Addgene Alerts box. ...

...this technological gap, a trio of recent papers describe the development of CRISPR activation (CRISPRa)...packaging capacity than AAV. Such an approach was described recently by the Day Lab, who developed neuron-...High-throughput screening strategies Although the tools described above make CRISPRa more accessible to neuroscientists... in vitro and in vivo applications, the tools described above make CRISPRa in the brain relatively easy...

...that the term ‘antibiotic’ can also be used to describe anti-parasitic drugs and anti-fungal drugs, because...not finishing the whole course of antibiotics prescribed to them This may seem counter-intuitive considering...The take-home? Always take your medication as prescribed. How do we know which bacteria are resistant ...infection is still effectively treated. We can prescribe antibiotics for the shortest time period possible...

...experience and skills would help you do the job as described. Let’s imagine you’re applying to a job that ... your cover letter, you can expand on that by describing how you developed and used a ddPCR assay to answer...exactly what you would want to talk about! You could describe your organizational system, your rationale for...company/organization]. I am a [adjective that describes you] scientist with over [number] years of experience...

... Addgene’s Plasmids 101: Aptamer Fluorophores describes the enrichment process used to evaluate oligonucleotides... of colors, so do aptamers! Corn was recently described in 2017 (Song et al., 2017). Corn naturally dimerizes...Corn under promoters for transcripts uniquely transcribed by RNA Polymerase III (5S, tRNA and U6) and measuring... Red and orange fluorescent aptamers Corn was described in the same study as Red Broccoli and Orange Broccoli...

...update, we have added new sections to share and describe some incredible new CRISPR technologies developed...much-anticipated prime editing section and additions describing CRISPR’s ability to make large genome edits using...transposases or the Cascade-Cas3 system. We also describe newly engineered Cas9s with more flexible requirements...

...minimizing confounding secondary effects? An approach described as “knocksideways” (a British idiom for “taking...two-color optical excitation of neurons. The paper describes five previously unknown channelrhodopsins from...Nature 2012 article by Jinny Kim and colleagues describes their efforts to map the location and distribution...mGRASP plasmids. Additionally, the lab recently described the use of another set of mGRASP plasmids in their...after SLIC cloning. The general cloning strategy described in the associated publication can be used to generate...

... discusses some of the programs SITN runs and describes some of the more popular topics from the Science...telling. Chapter 5: The SITN Blog - Vini and Amy describe how the SITN blog is a source of scientific information...underwater? Chapter 8: Work-Life Balance - Vini describes how she balances working in the lab and working...

...These tagging vectors are based on previously described pFA6a-link vectors and include a Kan, SpHIS5, ...former lab at UNC Chapel Hill. Bähler et al.(2) describe a modular collection of plasmids for a wide variety...3xHA, 13xMyc, GST, or GFP). Longtine et al.(3) describe a complimentary set of plasmids for use in S. ...

...transcription of downstream genes. Nihongaki et al describe the targeted activation of endogenous genes and...64543or 64542) When the reporter mRNA is first transcribed, it is bound by both the GFP and the RFP proteins...activation or repression. The TCS light sensors described in ACS Synthetic Biology (2014) represent the ...recent work in Nature Methods, Georgieva et al describe the rational production of a new series of epitope...several ways. The laboratory of Samie Jaffrey describes using these tags in an in-gel RNA visualization...articles every quarter so be sure to check in or subscribe to the blog to recieve the updates in our quarterly...

...CRISPR/Cas inspired genome editing was initially described by Cong et al. and Mali et al. in January 2013...Nine months later, more than 1500 publications describe work to improve the tool’s specificity, orthogonality...orthogonality, and multiplexibility in various species and describe an assortment of applications. Despite the ubiquity...