...update, we have added new sections to share and describe some incredible new CRISPR technologies developed...much-anticipated prime editing section and additions describing CRISPR’s ability to make large genome edits using...transposases or the Cascade-Cas3 system. We also describe newly engineered Cas9s with more flexible requirements...

...minimizing confounding secondary effects? An approach described as “knocksideways” (a British idiom for “taking...two-color optical excitation of neurons. The paper describes five previously unknown channelrhodopsins from...Nature 2012 article by Jinny Kim and colleagues describes their efforts to map the location and distribution...mGRASP plasmids. Additionally, the lab recently described the use of another set of mGRASP plasmids in their...after SLIC cloning. The general cloning strategy described in the associated publication can be used to generate...

...the particular conditions of the experiment as described in the associated article (listed below by PubMed...within promoters. When possible, the categories described on Addgene's CRISPR Plasmids and Resources page...

... discusses some of the programs SITN runs and describes some of the more popular topics from the Science...telling. Chapter 5: The SITN Blog - Vini and Amy describe how the SITN blog is a source of scientific information...underwater? Chapter 8: Work-Life Balance - Vini describes how she balances working in the lab and working...

...These tagging vectors are based on previously described pFA6a-link vectors and include a Kan, SpHIS5, ...former lab at UNC Chapel Hill. Bähler et al.(2) describe a modular collection of plasmids for a wide variety...3xHA, 13xMyc, GST, or GFP). Longtine et al.(3) describe a complimentary set of plasmids for use in S. ...

...CRISPR/Cas inspired genome editing was initially described by Cong et al. and Mali et al. in January 2013...Nine months later, more than 1500 publications describe work to improve the tool’s specificity, orthogonality...orthogonality, and multiplexibility in various species and describe an assortment of applications. Despite the ubiquity...

...transcription of downstream genes. Nihongaki et al describe the targeted activation of endogenous genes and...64543or 64542) When the reporter mRNA is first transcribed, it is bound by both the GFP and the RFP proteins...activation or repression. The TCS light sensors described in ACS Synthetic Biology (2014) represent the ...recent work in Nature Methods, Georgieva et al describe the rational production of a new series of epitope...several ways. The laboratory of Samie Jaffrey describes using these tags in an in-gel RNA visualization...articles every quarter so be sure to check in or subscribe to the blog to recieve the updates in our quarterly...

...retroviruses, reverse transcribe their gRNA into DNA, which is then transcribed into mRNA before being...depending on the class of virus. RNA genomes can be transcribed into mRNA, directly translated into proteins,...

...RTs in this regard and is even able to reverse transcribe the extremely long, highly structured HCV genome...structured template!). MarathonRT also faithfully transcribes RNA to DNA with low error rates compared to other...from A. victoria the functionalized nanobodies described here can be used to target any protein fused with...

... of the major CRISPR lingo and will be able to describe the functions of the various CRISPR/Cas9 components...Bind to a guide RNA The guide RNA, which will be described in more detail below, enables Cas9 to cut a specific...arrays as found in the bacterial genomes are transcribed into pre-crRNAs containing both the spacer region...

...plasmids containing the indicated gene, and you can subscribe to these pages to learn when Addgene receives ... articles reporting chromatin-related research describe the production of plasmids that might be useful...colleagues and guest bloggers have written posts describing new methods and tools for studying chromatin ...

...New work from Konermann et al. and Yan et al. describes new Cas13d enzymes that average only 2.8 kb in...Like other Cas13 enzymes, the Cas13d orthologs described in these papers can independently process their... high targeting efficiency, both of which are described below. Yan et al.: WYL-domain-containing accessory...

...adoption of the technology. In 2012, Saito et al. described the first Nano-lantern, a fusion of a mutagenized...hours! The latest enhanced Nano-lanterns (eNL) described in Suzuki et al. improve the brightness of previous...substrate furimazine. In addition to the previously described applications, the enhanced signal of eNL allows...

...27478040. The lung cancer mutant collection is described in this paper. Systematic Functional Interrogation...the genetic pathway reference collections are described in this paper Systematic morphological profiling...  The genetic pathway reference collection is described in this publication We also wrote an extensive...

...prone to degradation – those are the words that describe RNA! Double stranded? Well, that’s just for DNA... R-loops are particularly enriched in highly transcribed regions and repetitive regions, such as telomeres... and repetitive sequence. All of the R-loops described above play distinct roles in promoting cellular...

...enriched pool of sybody candidates are then reverse transcribed into cDNA fragments that are efficiently subcloned...for binding to the target protein. The protocol describes going through two rounds of phage display to increase...interest. The Seeger lab used the ELISA technique described in their protocol because it is less susceptible...

...following cloning methodologies have been extensively described in other articles; so if you are interested in... to assemble up to 6 different fragments in a prescribed order. These fragments can be pooled in one tube...homology. You might also consider FastCloning, first described by Chaokun Li and colleagues. Here you simply ...

...template with the endogenous gene. Here, we'll describe the techniques and the plasmids used to inactivate...Conditional knockouts The methods and plasmids described in the preceding sections are simple ways of knocking...knockout, researchers often use the Cre/Lox system described earlier. In this case, you design your targeting...

...world examples of these situations in cells are described below (See Figure 1).   Figure 1: Each... not necessarily monomeric, even if they were described as monomeric in a publication. The gold standard... [2]. Some affinity assays used in the papers describing the FPs above including molecular sizing columns...

...made news in March with a publication in Science describing the development of “transcriptors,” transistor-like... and observed that we humans find it useful to describe and interact with the world using words like OR...The future of synthetic biology Addgene: You've described the pieces in these kits as the beginnings of ...