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We narrowed to 494 results for: control

Showing: 41 - 60 of 494 results

  1. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...express CAs9 and are included as a control (WT). Vinculin is a loading control. 3. In terms of making the...undigested control. Sometimes the PCR products can look smeared, so having an undigested control can help...those formed from control (GFP gRNA-targeted) BRAF sites (lanes 3, 4) were used as controls. Duplexes were...). Anti-vinculin Western blotting is a loading control. Pro-Tips: 1. It’s all about the MOI. In retrospect...and BRDN0001146266) ( 76479-LV and 76480-LV) or control GFP gRNA (BRDN0000561167) (80034-LV) were individually... gRNA-transduced Cas9-A549 cells are used as a control. DNA was analyzed by PAGE. The basis of the...sequence), and PCR-amplify the BRAF locus from control cells (the PCR product should contain the wild-...

  2. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...right negative control is critical to the success of BiFC experiments. The ideal control is a mutated variant... work. You will also need to generate negative control constructs — more on this later. For more on constructing... measure the fluorescence of a single FP. Your control construct may generate a modest level of fluorescence...intensity of your BiFC constructs to your negative control to determine whether your PIPs actually do interact...has been compromised (Kodama and Hu, 2012). Such controls are necessary because split FPs can associate,...fragments themselves cannot be used as negative controls because their expression level, stability, and...structural information, constructing appropriate controls may requires that you empirically test a number...

  3. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...markers. Compensation/reference control(s) Compensation or “reference” controls are samples stained with a ...is not the same as your single color control. Reference controls: Beads or cells? Compensation beads are...mathematical algorithm and single stained reference controls to distinguish one fluorophore from another within...autofluorescence (you can see this in your unstained flow controls). Spectral flow cytometers include a tool called... or spectral flow. In spectral flow, reference controls tell the software to look for that exact signature...using conjugated dyes, it is critical that your controls and your sample antibodies are from the same batch...on a spectral cytometer, you prepare reference controls of both beads and cells for a single fluorophore...

  4. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    ...place the most important controls front and center, and make specialized controls available when needed....sequence and display frequently used genes and control elements. Development of this tool required creating... DNA sequence has changed. Identifying plasmid control elements Coding sequence features are relatively...relatively straightforward to define, but for control elements such as promoters and transcription terminators...obvious. We found that the annotated boundaries for control elements in commercial plasmids were inconsistent...how we use SnapGene during the plasmid quality control process Check out full plasmid sequencing at Addgene...

  5. Cre-Lox and Other Site-Specific Recombinases

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    Collection
    ...systems, how to use them to modify the genome or control gene expression, and find plasmids for your experiments...engineering tools used to precisely manipulate DNA and control the expression of specific genes in cells or organisms...recombinases can be used to modify the genome or control gene expression or skip ahead to browse highlighted...Summary of Cre, Dre, and Flp Recognition Sites. Controlling Recombinase Expression By expressing the enzyme...promoters or inducible systems, you can precisely control and optimize expression of your gene of interest... and C-terminal fragments and placed under the control of different promoters. Expression of both fragments...experimental system, enabling multi-layered genetic control. Recombinase-mediated cassette exchange (RMCE):...

  6. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...probability of artifacts. Off-target controls A seldom used control for targeted CRISPR experiments is ... how to predict and minimize them, and ways to control for and quantify them when they can’t be avoided...putative off-targets would be suitable. How to control for off-target events Sometimes there just isn’...applications, this is ok, but it still must be controlled for and acknowledged as a potential limitation...to perform key experiments on is a good way to control for off targets as well as clonal differences. ...your experiment is the only way to effectively control for off-targets and significantly decrease the ...effectively serve as a transfection or transduction control, a different gRNA would have distinct off-target...

  7. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...Photoactivatable Fluorescent Proteins Control Protein Expression with Cre-lox Control Protein Activity with GFP ...transmitted by intermediate messengers. Optical control of kinase activity can provide greater spatiotemporal...There is no common method for creating optically controlled kinases. The Solution: In Zhou et al, the Lin... the delayed effects because you can precisely control the timing of kinase activity. 2. Studying negative...photoswitchable kinases in vivo not only allows for precise control of a signaling pathway in the context of a whole...addition, it could be possible to use pdDronpa to control other kinases and proteins. Zhou et al suggest ...Pengpeng Li, Kang Shen, and Michael Z. Lin. "Optical control of cell signaling by single-chain photoswitchable...

  9. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

    Type
    Blog Post
    ...plasmids. This is because in 2024 certain export control exceptions were made by the US Bureau of Industry...Industry and Security, pursuant to the international controls prescribed by the Australia Group, for these plasmids...researchers in countries with applicable export control exceptions for rabies and VSV-G genes, plasmids...strive to keep our information updated, export controls and regulations are complex and may change suddenly...

  10. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...104–105 signal intensity. If your samples and controls were processed the same way, the GFP- population...population should overlap with the negative control population (Figure 1). Based on where the GFP- population... Example of yes-no flow plot of the (negative) control (left) or with the sample (right). Negative and...biorender.com. Pro tip! If your samples and control underwent different treatment processes, you may... population, so it no longer overlaps with the control. In tricky experiments, these populations may overlap...positions to the base expression. When compared to the control — which should be base expression — a peak to the...comparable expression to the baseline/negative control. You can see how it is easier to visualize these...

  11. Let There Be LITE Plasmids

    Type
    Blog Post
    ...populations. They demonstrated use of the LITEs to control gene expression in mouse neurons and in the brains...both quickly and significantly. Genes under LITE control showed a rise in transcription in as little as ...after light stimulation. Under those optogenetic controls, gene expression levels reliably increased in ...to establish it as “a novel mode of optogenetic control of endogenous cellular processes." The LITE system... Reference: Silvana Konermann et al. "Optical control of mammalian endogenous transcription and epigenetic...

  12. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...negative control, and a weak signal has a longer exposure time closer to the negative control. Reproducibility...specificity and exposure time. We first have a negative control which does not have primary and only contains secondary...background noise. We then compare the negative control to a sample with the primary included to confirm...If the sample images similarly to the negative control, that informs us that the primary antibody is very...binding. If the sample differs from the negative control, such as showing a very bright signal only in the...time of the primary antibody compared to negative control also determines the signal’s strength. Exposure...your sample to. For example, we use our negative control to compare exposure times of our samples. A prominent...

  13. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...high false positives. Control vs. all samples Oftentimes there exists a control group and multiple experimental...significantly greater or lesser than the mean of a control group. If you only care if one group is different... want to measure if a sample is greater than a control), then a one-tailed test is suitable. Of course...subjects or populations (a treatment group vs. a control group), on the other hand, should be analyzed with...group being compared to a standard value (not a control group). This situation is best served by a one-...can test three or more groups (two samples and a control, for example) simultaneously, with up to two independent...these tests from ANOVA/MANOVAs. Quantifying and controlling for any covariance between manipulated independent...

  14. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ...system along with precisely truncated attP sites to control recombination of the gene construct. A predictable...gene. Effector genes, any gene that you want to control the expression of using the SPARC system. The effector...rest of the SPARC components and expression is controlled by the upstream recombination outcome. Find ...Find SPARC plasmids at Addgene To more precisely control the amount of labeling, you can alter the attP site...effector position, as well as to optogenetically control the depolarization of R2 neurons using CsChrimson...genome engineering in flies Learn more about controlling gene expression in flies Resources on Addgene.org...

  15. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...highest quality, we undertake a rigorous quality control process. In 2016, we launched our viral service...our scientists perform all the standard quality control assays for AAVs. These steps include measuring ...Addgene is also conducting more rigorous quality control, including next generation sequencing (NGS) of ...VGS). Why should you care about viral quality control? AAV vectors are produced by scientists - not robots...Lecomte et al., 2015). This higher level of quality control is recommended but not yet required for clinical-grade...addgene.org to answer your questions about our quality control process! The images in this blog post were created...2011) Vector Characterization Methods for Quality Control Testing of Recombinant Adeno-Associated Viruses...

  16. Control AAV Preps

    Type
    Collection
    ...Packaging Service AAV Controls Viral Vector Packaging Service: Control AAV Browse our control AAV that express... select plasmids in the repository. Browse our control AAV collection, including fluorescent proteins,...consider using fewer filtering options. Browse all Control AAV *AAVrg = retrograde serotype, produced with...

  17. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...opposed to ordering control mice from a stock center. This serves as a better control since the experimental...knockout lines and Cre-lox lines, and how to properly control for genetically engineered mice in your experiment...not a waste. Best practice is to use littermate controls in your experiment - use mice that were bred in...experimental and control mice will have been raised in the same environment and will harbor similar epigenetic... in a tissue-specific manner (with expression controlled by a tissue-specific promoter). And, Cre may ...result of recombination at the floxed site, your control should be littermates that express Cre but not ...

  18. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...promoters can be regulated via positive or negative control. Positive Repressible: An activator protein is ...pJKR-H-tetR from the Church lab uses this system to control expression of GFP.)  To turn this inducible system...system, Gossen and Bujard created the tetracycline-controlled transactivator (tTA). They fused TetR with the...interest to be expressed. These permit exquisite control of gene expression and tracing of gene expression...that sometimes it is necessary or desirable to control or look at multiple genes at the same time.  The... genetic analysis in Drosophila or for precise control over multiple genes in synthetic systems. Recent...

  19. Serotype Testing AAV

    Type
    Collection
    ...Ready-to-use AAV available from Addgene's viral service. Control EGFP vectors in various serotypes for serotype ...list of all 100 µL sized sample, please see our Control AAV Inventory . The catalog ID for the 20 µL sized... catalog item, including the titer and quality control, visit the material page (linked for each catalog...

  20. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... of a magnetoresponsive fluorescent protein. Controlling an optogenetic or fluorescent tool with magnets... 2016), and some previous attempts at magnetic control of biological systems (Wheeler et al., 2016; Duret...T., York, A. G., Ingaramo, M. (2024). Magnetic control of the brightness of fluorescent proteins. Zenodo...Güler, A. D. (2016). Genetically targeted magnetic control of the nervous system. Nature Neuroscience, 19(...
Showing: 41 - 60 of 494 results