We narrowed to 718 results for: des.1

...collateral RNA degradation seen in bacteria. Figure 1: Overview of RNA targeting. Browse, sort, or search...regardless of homology to the crRNA. This activity provides a stark contrast to Cas9 and Cas12, which require...search the tables below for CRISPR plasmids designed for RNA targeting. To learn more about RNA targeting... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

...-Halo tag - Mammalian Gateway Destination Vector pENTR4-HaloTag (w876-1) - Gateway Entry Vector pCS2+/...Expression mEGFP 488 507 34 6 Monomer (A206K) pmEGFP-1 - Mammalian Expression mEGFP-N1 - Mammalian Expression...TurboRFP-pBAD - Bacterial Expression tdTomato 554 581 95 4.7 1 h Tandem-dimer pCSCMV:tdTomato - Mammalian Expression...-pBAD - Bacterial Expression mRFP1 584 607 13 4.5 1 h Monomer pcDNA3-mRFP - Mammalian Expression pMXs-...pSNAPf-C1 - Mammalian Expression pENTR4-SNAPf (w878-1) - Gateway Entry Vector pCS2+/C-SNAPf , pCS2+/N-SNAPf...fluorescence to reach half its maximal value. Structure describes the oligomeric state of the protein. Many FPs ...

...in mammalian cells or zebrafish). pMG10 (a '“2-in-1” ZFN cassette for expression of both a “+” and “-”...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays...Expression Vectors This page lists plasmid vectors designed to express nucleases in different cell types. ...zebrafish (can be used as a substitute for pMLM335 described in Foley et al., PLoS One 2009) pMLM292 (for expression...zebrafish (can be used as a substitute for pMLM336 described in Foley et al., PLoS One 2009) pMLM800 (for expression...Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al. PLoS One 2012 and Cade et al.,...

...for any application TMN200 (200 mM sodium chloride, 1 mM magnesium chloride, 20 mM Tris at pH 8.0) Suitable...adeno-associated viral vector. Hum Gene Ther Methods . Feb;25(1):72-82. PMID: 24191859 Ye, et al. 2015. Safety and...name for ordering information. ID Name Promoter Description Category PI 157970 pTR-UF11 chimeric CMV/Chicken...

... edits via homology-directed repair (HDR). Figure 1: Overview of Cas9 nickase cleavage and repair. Browse...PI Publication Return to top Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

...with a genomic region of interest in vivo. Figure 1: Overview of purification of genomic loci using CRISPR...or search the tables below for CRISPR plasmids designed for the purification of genomic loci. To learn... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

...marker. Looking at Table 1, we can recognize a couple of fluorophores from Figure 1. For example, the chart...brightness of individual fluorophores. Table 1 describes the brightness of a few commonly used fluorophores...efficient (i.e., happen most of the time). Figure 1 shows a graph from the BD® Spectrum Viewer, outlining...Resonance Energy Transfer (FRET).   Figure 1: Emission spectra and respective colors of a few commonly...Brighter fluorophores have a stronger signal. Table 1: Degrees of brightness for a few commonly used fluorophores...blue laser excites at a wavelength of 488 nm (Table 1), which is near a local maximum of PE's excitation...Knowing that the red laser excites at 640 nm (Table 1), while comparing the two fluorophore's excitation...

...with target sites throughout the chromosome. Figure 1: Overview of visualizing genomic loci using CRISPR...easy to implement due to the simplicity of gRNA design, programmable for different genomic loci, capable...or search the tables below for CRISPR plasmids designed for the visualization of genomic loci. To learn... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

...Buenrostro JD, Regev A, Zhang F. Cell 2023 Jan 5; 186(1):209-229. PubMed (Link opens in a new window) . TF...identification during screening. Library ID PI Description MORF library 137000, 192821, 1000000218 Zhang...

...Tris-tricine 2.5–40 kDa Tris-tricine 30 V, 1 hour or 100 V, 1–2 hours Good separation, quality, and stability...dive in!      Figure 1: The technical decisions to make when designing a western blot.  Psst!...assay. The most common options are 4 °C overnight or 1–2 hours at room temperature (RT). Most people choose...overnight at 4 °C and their secondary antibody for 1–2 hrs at room temperature, which allows them to reduce...weight of the proteins you’re interested in. Table 1 lists the most common types of gels for SDS-PAGE running...gel chemistry and/or specialty gel options. Table 1: Types of gels  Gel Type Protein sizes Running... Tris-glycine 6–400 kDa Tris-glycine 100 V, 1–2 hours Easy and cheap to handcast Short shelf-life...

...Morgens et al. contains three types of guides: 1) targeting guides (blue), which target all protein-coding..., the toxicity of guides with off-target sites was profiled. Guides with exact or 1-bp mismatch off-targets...) or short (17-18 bp) guides. When comparing the toxicity of guides that have 1-bp mismatch off-target... Figure 1: Summary of the advantages of the new approach to genome-wide CRISPR screen design.   Bassik... in both screens, with the growth screen having a 1% false discovery rate and identifying >88% of previously...characteristics that influence gRNA off-target activity: 1) mismatches closer to the PAM are less tolerated than...Knockout Library are available from Addgene! References 1. Morgens, David W., et al. "Genome-scale measurement...

...occurs at a much lower efficiency than NHEJ. Figure 1: Overview of NHEJ and HDR following cleavage by Cas9...or search the tables below for CRISPR plasmids designed to introduce a DSB. To learn more about NHEJ, ... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

...gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids 159654...can be designed using Benchling or using similar design tools and ordered as standard desalted oligonucleotides...phosphorylated oligonucleotides containing the guide target sequence: Up to two guides can be expressed...that utilize Golden Gate cloning to insert the desired guide RNA sequence by digestion of the backbone... #63798) Plasmids from Collection ID Plasmid Description PI Publications Kaufman, M. L., Goodson, N. B...

...Biology, 2(9), 537–541. DOI: 10.1021/sb400021j Figure 1: Multiplexing CRISPR knockdown to modulate expression...CRISPR guides. The first one binds to sfgfp with either 0, 10, 11, 14, or 20 matching nucleotides, the ...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The David Bikard Lab at...mcherry with either 0, 11, 18, or 20 matching nucleotides. Together, these plasmids allow to explore the...

...research and innovation programme and EFPIA. Figure 1: Representation of RESOLUTE mission. The combination...proteomic interactions represent the framework that provides biological context. Assays monitoring impedance...sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled...solute carrier family of proteins. Library ID PI Description Human SLC Activation Library 132561 Superti-Furga...

...activation-induced cytidine deaminase (AID). Figure 1: Overview of CRISPR base editing. Browse, sort, or...protein (Mu- GAM). While many base editors are designed to work in a very narrow window proximal to the... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

...~50% Table 1: Nanoblades editing efficiencies of primary cells. Comparing Nanoblades to Existing CRISPR...components into 293T cells. Figure 1: The key components of Nanoblades. Image from Mangeot et al., 2019....loaded with 1-4 different gRNAs. Viral envelope proteins To alter the cell tropism of Nanoblades, different... ~50-70% for these four cell types (Table 1). Additionally, hiPSCs and mouse BM cells still behaved like...mouse-model generation. Key components of Nanoblades Nanoblades are a Cas9 RNP delivery vehicle that’s based...) are expressed to pseudotype Nanoblades. The team found that Nanoblades pseudotyped with a mix of VSV-G...using Nanoblades? Plasmids are available from Addgene. Genome editing primary cells with Nanoblades Primary...

...for brain circuit mapping and connectomics. Figure 1: Reconstruction of a sparse set of neurons across ...light microscopy. In brain tissue, these protein barcodes allow for efficient machine-learning segmentation...enhancers for morphological readout. How to Use PRISM Barcodes are delivered as pooled AAVs either directly to...bias in packaging representation. To read out the barcodes, each protein tag can be imaged with a corresponding...combinations of protein bits. B) Multiplex imaging of barcodes with expansion microscopy and reconstruction of...Sheridan et al. (2025). PRISM Plasmids ID Plasmid Description Tags Additional Resources PRISM was developed...., & Payne, A.C. (2025). Combinatorial protein barcodes enable self-correcting neuron tracing with nanoscale...

...spike protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description Mutations Industry PI...138152 pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro Lentiviral vector expressing destabilized EGFP...neutralizing activity are listed below: ID Plasmid Description PI 44965 NL4-3 mCherry Luciferase Lentiviral ...

...vectors. PLoS ONE, 9(8), 1–10. https://doi.org/10.1371/journal.pone.0106472 de Backer, M. W. A., Brans,...promoters. Gene Therapy, 28(1–2), 56–74. https://doi.org/10.1038/s41434-020-0169-1 Oh, J. Y., Han, J. H., ...goals when designing and testing your AAV.  Figure 2: Comparison of AAV serotypes 1, 2, 5, 6, ...the selection process. Factors to think about are (1) your experimental conditions and measurements; (2...serotype, promoter, and titer.     Figure 1: Flow chart depicting a) steps for selecting serotypes...injection site than AAV2 (Watakabe et al., 2015) (Figure 1). You’ll need to consider if such properties could...Image modified from Aschauer et al., 2013, Figure 1. Used under Creative Commons Attribution License. ...