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We narrowed to 435 results for: erg

Showing: 41 - 60 of 435 results

  1. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post
    ... and what's it good for? Fluorescence resonance energy transfer (FRET) is a biophysical interaction between...fluorophore (the donor) is excited, it can transfer energy directly via resonance to the second fluorophore...fluorophore labeled and that these fluorophores can undergo FRET when they are near each other. How do you ...still develop a successful FRET-based assay since energy transfer only occurs when donor and acceptor are...acceptors >100 Å away from the donor doesn’t result in energy transfer and thus, your specific FRET signal is...donors never “see” FRET acceptors, and thus never undergo FRET. If this nonspecific labeling far exceeds ...experiments will define the maximum and minimum energy transfer values you can expect, thereby establishing...

  2. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...molecule is excited by a high-energy photon and re-emits a lower-energy photon; no chemical reaction takes...) worlds Forster Resonance Energy Transfer (FRET) is the transfer of energy from an excited donor fluorophore...Bioluminescence Resonance Energy Transfer (BRET) works the same way, except that energy is transferred from ...Fluorescence Source of light Chemical reaction High-energy photons Kinetics of photon generation Slower ...interaction brings the fluorescent protein close enough, energy is transferred by BRET and the fluorescent protein...

  3. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post
    ...have acquired divergent functions due to mutations accumulated over time? Undergraduates can help to answer...providing authentic research experiences to undergraduate students (1, 2), but educators face significant...Time project provides a framework for these undergraduate research questions. The idea is straightforward...cerevisiae. The two yeasts are thought to have diverged from a common ancestor ~350 million years ago.... have been largely conserved since the species diverged. Tips on using the Pathways Over Time plasmids... in strategies to incorporate research into undergraduate courses and in communicating science to the ...Advancement of Science. (2011). Vision and Change in Undergraduate Biology Education: A Call to Action, Washington...

  4. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...What is this FRET you speak of? Förster Resonance Energy Transfer (FRET) was originally described by Theodor... the alternative acronym Fluorescence Resonance Energy Transfer. Unlike the typical excitation and emission...fluorophore, FRET involves a non-radiative transfer of energy (i.e. no emitted photons) from the excited donor...fluorophore excitation by absorption of a photon 2. Energy transfer from the excited donor directly to the...spectrum of the acceptor fluorophore. Otherwise the energy transferred from the donor will not be able to ...of each other and be oriented appropriately for energy transfer via dipole-dipole interaction. The efficiency...M., et al. "Quantification of Förster resonance energy transfer by monitoring sensitized emission in living...

  5. Antibodies 101: Designing Your First Flow Panel

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    Blog Post
    ...the photon's energy is lost, so the energy of an emitted photon is lower than the energy of the absorbed...wavelengths are higher in energy, while photons of higher wavelengths are lower in energy. As such, wavelengths...they promote electrons within atoms to a higher energy state. This process is called excitation. After...a short period, the electrons revert to a lower energy state, whereby a photon is emitted. This step is... due to a process called Fluorescence Resonance Energy Transfer (FRET).   Figure 1: Emission spectra... relationship between a photon's wavelength and energy, how photons can be absorbed and emitted by matter...

  6. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...Reporter UnLOCKING (SHERLOCK and SHERLOCKv2) (Gootenberg et al., 2017 and 2018). While the Doudna lab ...not been approved for diagnostics yet, but is undergoing validation studies.     Jennifer Tsang contributed...updating this article. References Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DBT...439 . https://doi.org/10.1126/science.aar6245 Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy...442 . https://doi.org/10.1126/science.aam9321 Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins...10.1126/science.aaq0179 Myhrvold C, Freije CA, Gootenberg JS, Abudayyeh OO, Metsky HC, Durbin AF, Kellner...Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann...

  7. Meet the New Addgene Board Members!

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    Blog Post
    ...other reagents in biology. Luk H. Vandenberghe, PhD Luk Vandenberghe’s first lab experience was in a virology... Khandan Baradaran, Frank Stegmeier, and Luk Vandenberghe. We’re excited to grow the team and bring on...worked on vaccines throughout that time. Now Vandenberghe is an Associate Professor at Mass Eye and Ear... to develop an AAV-based COVID-19 vaccine.  Vandenberghe’s work with AAVs led to many interactions with...ultimately make innovation and science slow,” Vandenberghe says. “The continuous creativity that Addgene...Karen Guerin for the journal Molecular Therapy. Vandenberge is also the Grousbeck Family Chair in Gene Therapy...

  8. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...Plasmids: History & Biology The Joshua Lederberg Papers Joshua Lederberg's Personal Perspective: Plasmid (1952...Pubmed PMID: 13003535. Lederberg, Joshua, Luigi L. Cavalli, and Esther M. Lederberg. “Sex Compatibility ...did plasmids get their name? In 1952, Joshua Lederberg set out to clarify the classification of these...the Fertility, or F-factor discovered by Ester Lederberg in 1952 was noted to integrate into the E. coli...PubMed Central PMCID: PMC3785787. References: Lederberg, Joshua. "Cell Genetics and Hereditary Symbiosis...

  9. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...Bioluminescence Resonance Energy Transfer CRET= Chemiluminescence Resonance Energy Transfer    Interesting...well-known FRET, aka Fluorescence (Förster) Resonance Energy Transfer, technique which allows the detection ..."a distance-dependent physical process by which energy is transferred non-radiatively from an excited ... may be higher than the signal generated by the energy transfer. On the contrary, if you choose a pair... development of new fluorescent pairs and the emergence of faster measurement systems, this technique .... Sekar RB, Periasamy A. Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell...

  10. CASTing Off for New Shores in Human Genome Editing

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    Blog Post
    ...CRISPR-transposon system before another exciting new advance emerges! The programmability and targeting power of CRISPR...the directed evolution expertise of the Samuel Sternberg and David Liu labs. Evolving CASTs for new contexts...plasmids here! The new horizons of evoCAST In the emerging world of gene therapy, highly tailored treatments... H., Klompe, S. E., Ronda, C., Wang, H. H., & Sternberg, S. H. (2024). Bacterial genome engineering using..., S. E., Vo, P. L. H., Halpin-Healy, T. S., & Sternberg, S. H. (2019). Transposon-encoded CRISPR-Cas systems...L. H., Chen, A. T., Tolar, J., Osborn, M. J., Sternberg, S. H., & Liu, D. R. (2025). Programmable gene...

  11. Networking and Inspiring Young Scientists at Freshers Fairs

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    Blog Post
    ...summer internship information sessions to help undergraduate students with their applications. The society...society currently has 1,283 members, who comprise undergraduates, graduate students, postdocs, faculty members...The society encourages interactions between undergraduates and PhD students so that they can help support...society currently has 352 members, ranging from undergrad all the way up to professors. Oxford Biological...at Imperial College London for students in this emerging field. It has gained over 170 members in its first...or biological societies were you a part of in undergrad or grad school? How did these societies help (...

  12. A Needle in a Base-Stack: Cas9 Structural Biology

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    Blog Post
    ..., so it associates with random DNA sequences (Sternberg et al., 2014). The nuclease lobes are inactive...and active conformations (Bravo et al., 2022; Sternberg et al., 2015). The correct positioning of the ...before cleavage can occur (Jiang & Doudna, 2017; Sternberg et al., 2015).     Figure 8: Comparison...cellular recycling factors to release its handiwork (Sternberg et al., 2014). New discoveries on this topic are...and Computational Biology, National Institute of Allergy and Infectious Diseases. Resources and references...9), 839–843. https://doi.org/10.1038/nbt.2673 Sternberg, S. H., LaFrance, B., Kaplan, M., & Doudna, J.... 110–113. https://doi.org/10.1038/nature15544 Sternberg, S. H., Redding, S., Jinek, M., Greene, E. C.,...

  13. Cloning Mammalian Cells with the Agarose Method

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    ...blogger Iris Lindberg, Professor at the University of Maryland School of Medicine. In the Lindberg Lab we ...blogger Iris Lindberg, Professor at the University of Maryland School of Medicine. Iris Lindberg is a professor...PC12, Rin5F, Neuro2A and GH4C1, among others (see Lindberg papers in PubMed). While we found that nearly ...neural and endocrine cells. She can be emailed at ilindberg@som.umaryland.edu.   Additional Resources on the...

  14. Streaking for Single Colonies: The Streak Plate Challenge

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    Blog Post
    ... any bacteriologist’s skill set. So when Dave Westenberg taught this concept in his microbiology lab course...wear a mask. (@GermJuggler) February 13, 2021 Westenberg, a Professor of Biological Sciences at Missouri...by using a thicker bacterial suspension,” says Westenberg. In addition to streaking for single colonies...them into different strains of E. coli,” says Westenberg. During the transformation experiment, he plans...efficiency.  Aside from the Streak Plate Challenge, Westenberg’s course includes Winogradsky columns and characterizing...

  15. Which Fluorescence Microscopy Technique is Best for Me?

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    Blog Post
    ...fluorescent microscopy. Collimated (non-converging or diverging) excitation light exits the microscope ...its unrivaled contrast, fluorescence imaging has emerged as the dominant light microscopy contrast technique...GFP. Because red light has approximately half the energy of blue light, two photons of red light are needed...Although the excitation light is completely reflected, energy is propagated into the sample via an evanescent...the fluorophore’s interaction with an evanescent energy field. Lightsheet microscopes that project ultra-thin...

  16. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    Blog Post
    ...after undergrad] was to learn and speak the language of science. I didn’t come out of [undergrad] feeling...solidified some concepts that are heard often during undergrad. Lauren Flynn is the Addgenie behind our...doctor. After taking a year break in between undergraduate school and studying for the MCAT, she decided...description.” Our resident bagel provider, source of energy, and conversation master knows that although her...

  17. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    Blog Post
    ...science.   Acknowledgements Chan Zuckerberg Initiative Chan Zuckerberg Biohub Manuel Leonetti Ryan Leenay...Greg Dingle, a software engineer with the Chan Zuckerberg Initiative. We hereby announce the general availability... us your questions or comments to gdingle@chanzuckerberg.com. We're eager to hear your thoughts. Many...Greg Dingle is a software engineer with the Chan Zuckerberg Initiative, working on assignment at the Biohub...

  18. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    Blog Post
    ...Cressey is a PhD Student in the labs of Dr. Elke Mühlberger and Dr. Rachel Fearns at Boston University School...Medicine, Department of Microbiology, National Emerging Infectious Disease Laboratories.  She is interested...The power of reverse genetics. Springer Berlin Heidelberg, 2004. 1-41. PubMed PMID:15298166. 3. Hoenen,...21699921. PubMed Central PMCID: PMC3586226. 4. Mühlberger, Elke, et al. "Comparison of the transcription... 9971816. PubMed Central PMCID: PMC104478. 5. Mühlberger, Elke. "Filovirus replication and transcription...

  19. Transferable Skills Guide: Time Management

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    Blog Post
    ...skills, but I’ve learned a lot since my days as an undergrad working with cancer cell lines. As I started planning...useful tasks. The quick accomplishments will likely energize you. For example, let's say you’re doing a multi-day...that should help keep you content and give you the energy to finish any additional work you might need to... all if I’d planned appropriately. Back in my undergrad days, I had general goals for my experiments, ...to it each day. Once you get over the activation energy barrier that comes with making ANY progress, the...

  20. Hot Plasmids: Spring 2025

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    Blog Post
    ...proteins (GPC1, GPC3, and GPC4). All IPI antibodies undergo extensive characterization at IPI as well as community-led...CRISPR homing of retroelements." The Abudayyeh-Gootenberg Lab developed this tool based on several new ...Lim, J., Hiraizumi, M., … Abudayyeh, O. O., & Gootenberg, J. S. (2025). Reprogramming site-specific retrotransposon... The enhancers span all four major classes of GABAergic interneurons — those marked by parvalbumin (PV...lysosomal-associated membrane protein 5 (LAMP5) — as well as cholinergic (choline acetyltransferase, ChAT) neurons. The...
Showing: 41 - 60 of 435 results