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We narrowed to 1,052 results for: fer

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Showing: 41 - 60 of 1052 results

  1. Fluorescent Proteins: FRET

    Type
    Collection
    ...Resonance Energy Transfer (FRET) is a process by which energy is non-radiatively transferred from an excited...energy transfer mCerulean, mVenus, and Amber (mVenus Y67C) constructs for measuring energy transfer to multiple...Bioluminescence Resonance Energy Transfer (BRET) . Browse Addgene's Luciferase Plasmid Collection or the Promega...FLIM) or FLIM-FRET, where the increased energy transfer through FRET reduces the donor lifetime. FRET ...separately fused to a donor or acceptor molecule (also referred to as intermolecular or bimolecular FRET) or (...acceptor are both fused to the same protein (also referred to as intramolecular or unimolecular FRET). Learn...factors. When designing your experiment, be sure to refer to published reports and include appropriate controls...

  2. Promega Plasmid Collection

    Type
    Collection
    ...nonprofit, Addgene offers expert support, educational resources, and efficient material transfer solutions that...HaloTag, SmBiT, LgBiT, HiBiT, and more. Luciferase Reporters Luciferase (Link opens in a new window) reporter...NanoLuc luciferase-fusion protein expressed in cells. Additional Resources Browse Addgene's Luciferase Plasmid...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing...experimental workflows. The collection contains luciferase reporter vectors for sensitive gene expression...bacteria as an agar stab 20 µg purified DNA in Tris buffer (500 ng/µL ± 25 ng/µL) All plasmids in the collection..., reporter, protein, or tag, such as "NFAT", "luciferase", "Ras", "NanoLuc", "HaloTag", "BiT", or other...

  3. CRISPR Guide

    Type
    Collection
    ...Alternatives Resources Plan Your Experiment Web References PAM Sequences Glossary Publications CRISPR Overview...techniques . Collectively, these techniques are referred to as CRISPR ( C lustered R egularly I nterspaced...measure of fidelity, so sometimes they will be referred to as increased fidelity enzymes. hfCas9s were...pyrimidine-to-pyrimidine) Base transversions — edit to different classification of nucleotide base (purine-to-pyrimidine...opposite strands Prime editing and base editing both offer high efficiency and low indel formation compared...genetic changes, including moving entire genes to different locations, by combining other gene editing mechanisms...DNA-targeting of CRISPR-Cas systems. You may also see them referred to as INTEGRATE ( I nsertion of T ransposable ...

  4. Open Enzyme Collection

    Type
    Collection
    ...pOpen-EcoRIM M.EcoRI DNA methyltransferase 165517 pOpen-Eco31IA Eco31IA DNA methyltransferase 165518 pOpen-NotIM...pOpen-NotIM M.NotI DNA methyltransferase 165527 pOpen-Eco31IB Eco31IB DNA methyltransferase 165536 pOpen-PstIM...pOpen-PstIM M.PstI DNA methyltransferase 165538 pOpen-SfiIM M.SfiI 165538 DNA methyltransferase 165547 pOpen-SapIM1...pOpen-SapIM1 M1.SapI DNA methyltransferase 165557 pOpen-SapIM2 M2.SapI DNA methyltransferase 165558 pOpen-EcoRVM...pOpen-EcoRVM M.EcoRV DNA methyltransferase 165577 pOpen-XbaIM M.XbaI DNA methyltransferase 165578 pOpen-NcoIM ...pOpen-NcoIM M.NcoI DNA methyltransferase 165520 pOpen-T4 PNK T4 PNK polynucleotide kinase 165521 pOpen-lambda red...red operon 165530 pOpen-T4-BGT T4-BGT beta-glucosyltransferase 165531 pOpen-UDG UDG Uracil DNA glycosylase...

  5. Lentivirus Plasmids

    Type
    Collection
    ...pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase 3rd Expression of EGFP-Firefly luciferase fusion protein. Ethan Abel...envelope, and transfer plasmids Viral... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Transfer Plasmids Packaging...a virion) are divided among multiple plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA ...third-generation lentiviral packaging, envelope, and transfer plasmids available at Addgene. Read our Lentiviral...safety. Lentiviral Vector Guide Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids to express... 3rd U6-driven shRNA empty plasmid. Includes a stuffer for easy cloning. David Root 14748 pLKO.3G 3rd ...

  6. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...listing of transfer status for viral preps.... Viral Vector Packaging Service Penn Vector Core Transfer Viral ...Viral Vector Packaging Service: Penn Vector Core Transfer The University of Pennsylvania Vector Core (Penn...inventory , which includes all viral vectors transferred from the Penn Vector Core. Chemogenetics (including...mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-50943 50943-...mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68717 68717-...pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68719 68719-...

  7. Zhang Lab CRISPR Page

    Type
    Collection
    ...to LacZ, plus luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase...plasmid expressing SpCas9 alone without sgRNA. Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase...Library CRISPR page for more information. Full references are below. Return to top Cas9 SAM transcriptional...The MS2-P65-HSF1 activation helper protein Full references are below. Pooled genome-wide human SAM library...Swiech et al., Nature Biotechnology 2014). Full references are below. Available plasmids are described below...The vector can be digested using BsaI ( This is different from BbsI, which is used in the SpCas9 plasmid...with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin mouse can be purchased...

  8. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Vector Core Viral Vectors Lentiviral Transfer Vectors Adenoviral Transfer Vectors Resources Kazuhiro Oka and...lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this set of...packaging these transfer vectors and generating new viral vectors. Download the Lentivirus Transfer Vector Production...from the PGK promoter Adenoviral Transfer Vectors The adenoviral transfer vectors contained within this ... of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've efficiently...coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP...Flp recombinases. Genes can be cloned into these transfer vectors using shuttle vectors pICPIS-EF1 (Addgene...

  9. Optogenetics AAV Preps

    Type
    Collection
    ..., 5 Ofer Yizhar 198511 pAAV_hSyn-DIO-PdCO-mScarlet-WPRE Syn PdCO mScarlet Cre dependent 1, 5 Ofer Yizhar...GtACR2 (soma-targeted) FusionRed Constitutive 1, rg* Ofer Yizhar 105677 pAAV_hSyn1-SIO-stGtACR2-FusionRed ...soma-targeted) FusionRed Cre dependent 1, 5, rg* Ofer Yizhar 105678 pAAV_hSyn1-SIO-stGtACR1-FusionRed ...GtACR1 (soma-targeted) FusionRed Cre dependent 5 Ofer Yizhar 109048 pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1...mScarlet-WPRE CaMKII eOPN3 mScarlet Constitutive 1, 5, rg* Ofer Yizhar 125713 AAV-hSyn1-SIO-eOPN3-mScarlet-WPRE ...mScarlet-WPRE Syn eOPN3 mScarlet Cre dependent 1, 5 Ofer Yizhar 139504 pAAV-CAG-DIO-PPO-Venus CAG PPO Venus Cre dependent...pAAV_hSyn-PdCO-EGFP-WPRE Syn PdCO EGFP Constitutive 1, 5 Ofer Yizhar 198516 pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE...

  10. Mammalian RNAi Tools

    Type
    Collection
    ...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...Tools for RNAi Browse Plasmids Resources RNA interference (RNAi) is an RNA-mediated gene silencing mechanism...as shRNA (short hairpin RNA) or siRNA (short interfering RNA). Delivery can occur via transfection or ...using viral vectors. Lentiviral vectors are a preferred method for long-term gene silencing. Once the ...below. Figure 1: Overview of shRNA-mediated RNA interference. Created with BioRender.com. shRNA Plasmids ...pSico with pSicoligomaker (Andrea Ventura lab) References Carthew, R. W., & Sontheimer, E. J. (2009). Origins...J. (2009). siRNA vs. shRNA: Similarities and differences . Advanced Drug Delivery Reviews, 61 (9), 746...

  11. Antibody Production

    Type
    Collection
    ...formulation buffer is phosphate-buffered saline containing 1 mM sodium azide. This buffer is not compatible...columns. Formulation Buffer After affinity chromatography, antibodies undergo a buffer exchange using centrifugal... cells and will interfere with conjugation. For applications such as these, the buffer can be removed ...webpage. Antibody preparations developed at IPI may differ from Addgene antibodies in “Production & Usage”...extracted from cells, separated by SDS-PAGE, transferred to a membrane, and labeled with the antibody ...

  12. Retrovirus Plasmids

    Type
    Collection
    ...Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression with luciferase expression. Scott ...and transfer plasmids. Viral... Viral Plasmids Gamma-Retroviral Plasmids Gamma-Retroviral Plasmids Transfer Plasmids... been split into multiple plasmids for safety: Transfer plasmid — contains transgene, sgRNA, or shRNA ...collection highlights popular gamma-retroviral transfer, packaging, and envelope plasmids. Read our Gamma-retroviral...Gamma-Retroviral Vector Guide Popular Gamma-Retroviral Transfer Plasmids Want a retroviral plasmid with your gene...of interest? Search our database for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description...

  13. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ... CRISPR Interfere CRISPR Plasmids: Interfere Mammalian Bacteria Plant Yeast Resources...knock down gene expression by interfering with transcription. This interference is most efficient when the...Overview of CRISPR interference. Browse, sort, or search the tables below for CRISPR interference plasmids. To... To learn more about CRISPR interference and other CRISPR topics, read our CRISPR Guide . Mammalian ID...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

  14. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ... mCherry Luciferase Lentiviral dual reporter vector expressing mCherry and firefly luciferase to simultaneously...105621 Lenti-luciferase-P2A-Neo Lentiviral reporter vector expressing firefly luciferase with neomycin...firefly luciferase and ZsGreen. Alejandro Balazs 170674 pBOBI-FLuc Express firefly luciferase. Used in...David Nemazee 170575 pCMV-FLuc Express firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay...-GFP-Luc Lentiviral vector expressing GFP and luciferase for use as a reporter in pseudovirus production...

  15. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...for a different analyte, a sensor for gene-specific activity, or for popular biosensors in different expression...protein (cpFP) or fluorescence resonance energy transfer (FRET) efficiency of a pair of fluorescent proteins...the articles linked below include variants with different binding affinities or negative-control constructs...environmental factors and the sensor behavior may differ from the original characterization (e.g. in vitro...the plasmids associated with the article. We also offer ready-to-use AAV preparations of select plasmids...cGMP (cyclic GMP) FlincG GFP-based cGMP sensor Differential patterning of cGMP in vascular smooth muscle...chloride sensitivity, photostability and reduced ph interference demonstrates augmented transmembrane chloride...

  16. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...service gives you even more options. AAV Transfer Plasmids Transfer plasmids encode the genetic information...Browse AAV transfer, packaging, and helper plasmids in Addgene's repository. Viral...Plasmids Adeno-Associated Viral (AAV) Plasmids AAV Transfer Plasmids AAV Packaging Plasmids AAV Helper Plasmids...Plasmids Adeno-associated viral (AAV) vectors offer precise delivery of genetic material across a wide ...vectors, researchers need to use three plasmids: A transfer plasmid (or cis plasmid) containing the transgene...This collection provides a curated selection of transfer, packaging, and helper plasmids that can be used... This table contains a general list of all the transfer plasmids in Addgene's repository. You can search...

  17. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...formulated in one of the following three buffers. All buffers are supplemented with 0.001% Poloxamer 188... or 0.014% Tween 20. Phosphate Buffered Saline (PBS) A universal buffer suitable for any application Balanced...as blood, vitreous, CSF, etc. Not suitable as a buffer for vectors delivered directly to the brain parenchyma...Shannon Boye’s lab at the University of Florida. References for each serotype can be found below under Citations...is available in each of the above serotypes and buffers. Please click on the plasmid name for ordering ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

  18. Neurodegeneration Research Collection

    Type
    Collection
    ... to target different cell populations in many brain regions. See More iPSC Differentiation Factors Find...Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other...Amyotrophic lateral sclerosis (ALS), sometimes referred to as Lou Gehrig’s disease, is a progressive disease...are prone to misfolding and aggregation and can interfere with protein-protein interactions and nerve cell...of these are due to a mutation in one of three different inherited genes: Presenilin 1, Presenilin 2, and...expression constructs: pLVX-UbC-rtTA-Ngn2:2A:EGFP (Schafer et al. Nat Neurosci. 2019) pLVX-UbC-rtTA-Ngn2:2A...progression of Huntington’s disease. The foundation offers curated information on tools and reagents (Link...

  19. Tetracycline Inducible Expression

    Type
    Collection
    ...transcriptional and proliferative state of each cell in a population. Proliferation history is monitored...express both components in the same relative ratio. Different generations of transactivators and promoters are...new window) for comparison of Tet-On systems in different applications. Use of Tetracycline or a Derivative...Doxycycline, a derivative of tetracycline, is a preferred effector for tet-responsive systems. Dox binds...pSBtet-GP Sleeping Beauty transposon system with luciferase in cloning site. See article ( Kowarz et al.,...172115 PB-TO-hNGN2 Piggybac Tet-On plasmid for differentiating iPSCs into glutamatergic neurons via human ...NGN2 into AAVS1 safe harbor site, for iPSC differentiation into cortical neurons Michael Ward 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA...

  20. Viral Vector Guides and Plasmids

    Type
    Collection
    ...more about the different types of viral vectors and find viral production and transfer plasmids for a ...Gamma-retroviral Plasmids Gamma-retroviral vectors, often referred to as just retroviral vectors, have a relatively...Viral Vector Type Each viral vector type exhibits different properties and are suited for specific research...goals. For example, AAV vectors are typically preferred for in vivo studies because of their low immunogenicity...containing the genetic information to be delivered (transfer or shuttle plasmid) and one or more plasmids containing...Specific modifications to these plasmids, such as different promoters or tags, can even direct delivery of...
Showing: 41 - 60 of 1052 results