...pPS808 - N-terminal GFP for yeast expression pET GFP - C-terminal GFP for...gene expression GFP Varies pBABE GFP - Retroviral gene expression pMKO.1 GFP - Retroviral ... cassette pBTG (pBigT-IRES-GFP) - pBigT modified to contain IRES-GFP Hynes Lab...C elegans expression GFP Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression... from the SP6 promoter pCS2 mt-GFP - Create myc-tag, GFP-tag fusions for transcription ...expression pcDNA3 GFP LIC cloning vector - C-terminal GFP for mammalian expression...expression MSCV-IRES-GFP - Mammalian retroviral gene expression with GFP selectable ...

...markers were not increased compared to a control AAV-GFP. Not only did the AAV-SaCas9-sgRNA constructs mediate...genes in human cells using compact expression cassettes. Sci Rep 5: . https://doi.org/10.1038/srep10777...

...peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles ... This makes it possible to use a polycistronic cassette that expresses multiple reprogramming factors ...

..., most of the commonly used cassettes, such as fluorescent proteins (EGFP, mCherry etc.), recombinases..., we’ll use the example of inserting a 2A-FlpO cassette into the Fgf8 gene. Miura et al. (2018) suggest...

...vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human CD2 (...cloning All-in-one plasmid containing expression cassette for NmCas9 and BsmBI site for cloning in gRNA:...Musunuru CRISPRs in human pluripotent stem cells pCas9_GFP ; gRNA empty vector Link O'Connor-Giles Fly: gRNA...plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF 2.3 MB Zhang GeCKO pooled library amplification...packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Kits are also available (mouse or human libraries...

...Expression pRSETA-PAGFP - Bacterial Expression tandem PA-GFP-KanR - Yeast Expression pFA6a-PA-GFP-kanMX6 - Yeast...Expression pFA6a-link-yEPAGFP-CaUra3 - Yeast Expression mPA-GFP-N1 - Mammalian Expression mPA-GFP-C1 - Mammalian...then scientists have engineered numerous GFP-variants and non-GFP proteins that result in a diverse set ... multicistronic expression of GFP and/or mCherry, with neomycin cassette Zebrafish, Sea urchin, Xenopus...tagging with mCherry, mOrange, mCerulean pET GFP - C-terminal GFP for bacterial expression Davidson Lab Plasmids...Expression EGFP 488 507 34 6 Prone to dimerization pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian...Mammalian Expression PA-GFP 504 517 14 Monomer (A206K) pPAGFP-N1 - Mammalian Expression pPAGFP-C1 - Mammalian ...

...experiment, consider co-transfecting with GFP. This allows you to sort for GFP-positive cells and to enrich for...for example, plasmid with a puromycin resistance cassette, such as PX459 ). After you co-transfect the CRISPR...

...enough to carry the Cas9 gene and a gRNA expression cassette in one single viral particle. The earliest explorations... pAdSh.PGK.Cas9, pAdSh.U6.gRNAS1, and pAdSh.U6.gRNAGFP. Gonçalves says that advantages of AdVs include...guide RNA constructs, pAdSh.U6.gRNAS1 and pAdSh.U6.gRNAGFP, and also Adeno Cas9 and Adeno EA. Or if you're...

...another marker, such as GFP. A researcher can use FACS to sort cells expressing GFP and later expand these...DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB survival strain...

...Knock-in tagging - Michel Bagnat lab. Targeting cassettes encoding fluorescent proteins for tagging genes...-terminal tags, including Myc, HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible Kit ...

..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They...with unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many ...backbone to target a CAG-driven loxP-stop-loxP cassette into the Rosa26 locus, allowing for Cre-dependent... of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 amino-acid linker (see Figure 1). The...

...Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca+ dynamics in endoplasmic reticulum GFP-Aequorin Protein...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ...2020 GENIE Project Calcium jGCaMP7 High-performance GFP-based calcium indicators (Constitutive or Cre-dependent...Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing... FLAMP Plasmids Jun Chu cGMP (cyclic GMP) FlincG GFP-based cGMP sensor Differential patterning of cGMP... vascular smooth muscle cells revealed by single GFP-linked biosensors. Proc Natl Acad Sci U S A. 2008...