SunTag and Fluorescent Imaging
Type
Blog Post
...SunTag signal allowed them to cut their laser power by over 80% and still obtain a higher signal-to-noise...transcriptional activation. Multiple nuclear localization signals (NLS) increase protein import into the nucleus....lesson and created a system to amplify fluorescent signals. Named for the "stellar explosion SUperNova," SunTag...have to increase your imaging time to get enough signal. This workaround risks cellular phototoxicity and...short epitope GCN4. GCN4 recruits GFP fused to the cognate scFV antibody, which is expressed from a separate...system amplifies the intensity of the fluorescent signal and enables single molecule tracking within living...traditional fluorescent proteins
Improved brightness and signal-to-noise ratio
Less chance of phototoxicity
Reduced...