We narrowed to 416 results for: iCre

...87421 TUBA1B-mEGFP AICSDP-4 mEGFP Alpha-tubulin Microtubules 87422 LMNB1-mEGFP AICSDP-10 mEGFP Lamin B1 Nuclear...-mTagRFP-T AICSDP-28 mTagRFP-T Alpha-tubulin Microtubules 101786 ST6GAL1-mEGFP AICSDP-26 mEGFP Sialyltransferase...

...gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes...

... transduction of hematopoietic stem cells and microglia. The AAV6(dbY-F+T-V) serotype was developed by...6:35495. PMID: 27759036 Rosario, et al. 2016. Microglia-specific targeting by novel capsid-modified AAV6...

...-like transposases found in diverse species of microbes. However, synthetic dCas9-transposase fusions ...customizable DNA or RNA label for fluorescence microscopy. RNA aptamers, like MS2 or PP7, can also be fused...classification of CRISPR–Cas systems. Nature Reviews Microbiology , 13 (11), 722–736. PMID: 26411297 Marino, N...diverse bacteria with Mobile-CRISPRi. Nature Microbiology . 4 (2), 244–250. PMID: 30617347 Qi, L. S., ...

...244863 pLentiCRISPR V2 puro HTRA2 sg1 HTRA2 hU6 Parkinson's Alexis Jourdain 244864 pLentiCRISPR V2 puro...Jourdain 246017 pLentiCRISPRv2-sgFXN-1 FXN CMV Friedreich ataxia Vamsi Mootha 246018 pLentiCRISPRv2-sgFXN-2 FXN...Familial British dementia Jan Johansson 138347 lentiCRISPR v2-sgDnmt1_1 DNMT1 U6 Hereditary sensory neuropathy...neuropathy type IE Yi Zhang 138348 lentiCRISPR v2-sgDnmt1_2 DNMT1 U6 Hereditary sensory neuropathy type ...LRRK2 His T7 Parkinson's Suzanne Pfeffer 186025 lentiCRISPR v2-sgCOQ2 COQ2 U6 Multiple system atrophy Boyi...pET-Duet_OPTN-thrombin-GST OPTN GST T7 ALS Sascha Martens 218527 plentiCRISPR-v2 Puro EIF2AK2/PKR_sgRNA EIF2AK2 U6 VWM disease...

...-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9... of Cre recombinase. Madeline Lancaster 198752 DiLiCre 2.0 Dox-inducible expression of a light-activated...

...cells? 1. Epifluorescence microscopy This technique uses a fluorescence microscope where the light source...proteins or dyes. 3. Two-photon microscopy Two-photon excitation microscopy is a fluorescence imaging technique...regarding immune cell mobility than the static microscopy images of chemically coloured lymphoid organs...specimen and the excitation light passes through the microscope objective lens on its way toward the specimen...occurs only at a chosen focal volume and thus the microscope captures only fluorescence coming from this volume...below for an example of two-photon intravital microscopy from PLoS Pathogens - Kamenyeva et al, 2015. ...particular stimulus. Using an epifluorescence microscope and GFP fusion proteins controlled by promoters...

...limits the use of microscopy in open science. But the new field of open-source microscopy seeks to change... Open-Source Microscopy Sharing Hardware vs. Sharing Software In the field of microscopy, where image ...Stirling, et. al, "Robotic microscopy for everyone: the OpenFlexure microscope," Biomed. Opt. Express 11...Since its early years, the light microscope has been a powerful tool in almost all disciplines. In biology...experiments. Projects such as the OpenFlexure Microscope (Collins, et. al., 2020), the miCube, SQUID (...openUC2 (Diederich et. al., 2020) aim to make microscopy as a tool accessible to practically everyone....started my PhD, I have been convinced that most microscopy techniques can be replicated with commercially...

...competition between microorganisms for available resources. This competition drives microbes to develop survival...mechanisms. Some microbes even make their own antibiotic compounds. In such a case, the microbe itself would...surrounding microbes would be affected (possibly killed), thus increasing the initial microbe’s chances of...to Use Our Microbiology Pages Additional Resouces on Addgene.org Check Out Our Microbiology Pages ... guest blogger, Jennifer Rivers, a research microbiologist specializing in bacteriology and drug resistance...positive force for public health. Visit Addgene's Microbiology Pages What is antibiotic resistance? Antibiotic...Antibiotic resistance is defined as microbial survival despite exposure to antibiotics designed to kill them...

...  Opto-katanin: a light-powered microtubule-cutting sword Microtubules are one of the most important components...recruited to microtubules under blue light, removing tubulin dimers from the microtubule body. B) Schematic... several strategies are available to disrupt microtubules in cells, they have various disadvantages and...and limitations – for example, microtubule-destabilizing drugs are impossible to target to specific subcellular...published a new optogenetic tool for localized microtubule disassembly, Opto-katanin (Meiring et al., 2022...Figure 1: Opto-katanin induces fast, localized microtubule severing. A) Schematic of Opto-katanin constructs...under CC-BY 4.0 license. A naturally occurring microtubule-severing protein, Katanin is of course named ...

...track them. We have a number of microscopes, including an automated microscope but we need to develop the ...skills at the regular microscope, and the scripting skills at the automated microscope  to keep our worms...Working on the Cat Microbiome Project Another project we are working with is the cat microbiome project. This... domesticated cats and learning about feline microbiomes. One of the aspects of this project that makes...project gives them access to rich and complex microbiome data for analysis. And, well, cats... everyone...possible, but one of our members took an Arduino microcontroller and a motor controller and made it spin again...re-use it as a platform for monitoring homemade microfluidic chips using the fantastic optics and fluidics...

...the region between the microhomology and the retention of a single microhomology sequence. For more mechanistic...Step 1: Generate microhomologies in the PITCh vector ~20 bp 5’ and 3’ microhomologies are added to the...double-stranded break repair mechanism: MMEJ (microhomology-mediated end-joining)? MMEJ, a form of alternative...contrast, MMEJ uses regions with 5-25 bp of microhomology flanking a DSB to repair DNA. The DNA ends are...precise deletion of the sequence between the microhomologies.MMEJ has certain advantages over HDR. Some species...vector should be constructed with 5’ and 3’ microhomology to the insertion locus flanking the GFP-Puro... cassette and one in between the 5’ and 3’ microhomologies in the genomic locus. The first two breaks ...

...specific targeting of microglia with AAV By Alyssa Neuhaus Genetic modification of microglial immune cells is...valuable tool for microglia research and broader therapeutic applications.  Find microglia-targeting AAV ...Voltron2 voltage sensors AAV vectors for targeting microglia The REVeRT Dual AAV Vector System Neurodegeneration...for central nervous system research; however, microglia are notoriously resistant to viral transduction...IBA1 promoter (hIBA1a, 466 bp) that drives high microglia-specific gene expression when incorporated in ...Efficiency and specificity of these AAV vectors for microglia was further enhanced by packaging the virus as...transgene expression in neurons and other non-microglia cells.      Figure 5: A) Representative...

...the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab... and how does it work? LentiCRISPR v2 purpose As its name suggests, lentiCRISPR v2 is a lentiviral transfer...,000 human and mouse genes respectively, use lentiCRISPR v2 as the backbone for their many gRNAs. The ...high titer lentivirus production afforded by lentiCRISPR v2 is key to its efficient use in screening experiments...pooled library screens. Availability and Use LentiCRISPR v2 is available from Addgene both as a single...CRISPR/Cas9. If you need help cloning a gRNA into lentiCRISPR v2, you can take a look at the protocol kindly...kindly provided by the Zhang lab. Even though lentiCRISPR v2 has only been available for a little over a ...

...PubMed Central PMCID: PMC3984138. Microscopy U. Basics of FRET Microscopy. Additional Resources on the Addgene...Visualization can be achieved using fluorescent microscopy - even in living cells and whole organisms. You... many Fluorescent Proteins (FP) available for microscopy, by cloning your protein of interest into a vector...must bear in mind that the resolution of light microscopy is limited by the wavelength of light and, practically...practically speaking, for common lab microscopes the detection limit is 200 nm. The size of most proteins...10 nm and therefore colocalization detected in microscopy cannot be interpreted as direct molecular interaction...without further investigation. One fluorescent microscopy method commonly employed for analyzing interactions...

...technology can have on the human microbiome. You can consider the microbiome as a collection of organisms,... honey bee and bumble bee gut microbiomes titling it the Bee microbiome toolkit (Leonard et al., 2018)... Pseudomonas syringae and Klebsiella oxytoca. Microbiome engineering Scientists have begun to realize ...methods of introduction. Engineering the bee gut microbiome Beyond the human gut, the Barrick lab has deposited...plasmids that can be used to engineer the bee gut microbiome. They created a modular system using a broad ...-Cas nucleases to produce sequence-specific antimicrobials. Nat Biotechnol 32:1146–1150 . https://doi....RJ, Mimee M, Lu TK (2014) Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases...

...experienced this at the ASM General Meeting (now Microbe) in 2013 in Denver, CO. While I had been to the...scientists and some rising stars in the world of microbiology, some before they had even established their...Porch with old and new friends while attending ASM Microbe in Atlanta, GA, in 2018. A lot of my experiences...from participants in Binning Singletons at ASM Microbe in 2019 were overwhelmingly positive. Susanna Harris...remarked that “This was not my first time at the ASM Microbe meeting, but it was my favorite because I finally...better Our experience running this program at ASM Microbe in 2019 is written up in mSphere. A post-meeting...future to match people within similar fields (ASM Microbe has eight tracks), something we didn’t have enough...

...imaged in distinct fluorescent channels on the microscope. With the proliferation of fluorescent proteins...and that can be imaged with the optics on the microscope(s) you intend to use. An accurate determination...your fluorescent proteins compatible with your microscope optics? To determine if the fluorescent proteins...you are interested in are compatible with your microscope optics, you will want to compare the excitation...protein with the filter sets or lasers on your microscope. Ideally, you would like to have substantial ..., so that the protein is well excited by your microscope and the fluorescence emission of the protein ...protein is efficiently collected by the microscope. To compare the match between a fluorescent protein and a...

...with BioRender.com. Resistance (R) plasmids: microbial defense Genes allowing bacteria to survive toxins...resistance to them are the tools of a millennia-old microbial war that humans only noticed last century! Nevertheless...implications for public health. Bacteriocin plasmids: microbial offense One way to triumph over your neighbors...plasmids” or “Col factors.” Virulence plasmids: microbial infection Virulence plasmids encode genes that... transform plant cells. Degradative plasmids: microbial hardiness There are also less aggressive ways ...of metabolic pathways. Fertility (F) plasmids: microbial sharing F plasmids contain the genes for bacteria...through a population of cells. Cryptic plasmids: microbial mysteries Cryptid hunters watch out — cryptic ...

...home edition, the MicrobiGals created glow in the dark petri dish ornaments and microbe cookies! 🤗 🤗 ...Td2M6AOpuW pic.twitter.com/ofLI4eD8Zs — Microbigals (@MicrobiGals) December 23, 2020   In second place,...bacteria, fungi, viruses, and one microbe ornament even dyed with real microbial pigment. Check out the hashtag...place, Dr. Anne Madden created a microbe advent tree displaying over 25 archaea, bacteria, fungi, and viruses...viruses. Final tree with the complete #MicrobeAdventCalendar ornament set (plus more!) It features archaea...