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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 532 results for: invs

Showing: 41 - 60 of 532 results

  1. Biosensor AAV Preps

    Type
    Collection
    ...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Our ...

  3. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...window) Qiagen , (Link opens in a new window) Invitrogen , and (Link opens in a new window) Promega sell...fully resuspended. Add 200 μL of Solution II and invert the tube carefully 5 times to mix the contents.... μL of cold Solution III to each tube. Mix by inverting several times. A white precipitate will be formed...reactions. Air dry the pellet (can be done by inverting the tube at an angle over kimwipe) for 20-30 min...ethanol and 1/10 volume of 3 M Na-acetate (pH 4.8). Invert the microfuge tube to mix. Optional: Place the ... Pour out the supernatant in the sink. Open and invert the tubes on a paper towel to drain them out. Wash...about losing the pellet. Dry with vacuum or by inverting over paper towel for 5-20 min. Resuspend dry DNA...

  4. Antibody Guide

    Type
    Collection
    ...fluorophores used is important. It is best to inversely pair the brightness of the fluorophore with the...

  5. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ... 1mm thick, 10-well, Invitrogen NP0321BOX 20X MOPS SDS running buffer, Invitrogen NP0001 4X NuPage sample...buffer, Invitrogen NP0007 10X NuPage sample reducing agent (500 mM dithiothreitol, DTT), Invitrogen NP0009...NP0009 PageRuler Plus Prestained protein ladder, Invitrogen 26619 Pipette tips, 10 µL, VWR 76322-132 Pipette...MOPS buffer into 475 mL of deionized water. Gently invert to mix. Procedure Section 1: SDS-PAGE Add 5 µL ...

  6. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...Tween-20 to into 999.5 mL 1X PBS Cap the bottle and invert several times to mix. Carefully remove the plate...250 mg of BSA to 25 mL of PBS. Cap the tube and invert several times to mix. Using a multichannel pipette...Dilute 50 mg BSA into 50 mL PBS . Cap the tube and invert several times to mix. Dilute the primary antibody...three concentrations between 1–10 µg/mL. Cap and invert the tubes several times to mix. Using a multichannel...concentrations in the antibody dilution buffer. Cap and invert the tube several times to mix. Using a multichannel...Solution from the TMB substrate kit. Cap the tube and invert several times to mix. Using a multichannel pipette...

  7. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...serum-free media Invitrogen: #31985 Dulbecco’s Modified Eagle Medium (DMEM) Invitrogen: #11995 Fetal Bovine...Bovine Serum (FBS) Invitrogen: #16000 Penicillin/Streptomycin Invitrogen: #15140-122 Polypropylene tubes... buffer NEB #B0202S DH5 alpha competent cells Invitrogen #18258-012 Qiaquick gel extraction kit Qiagen...homologous recombination of unstable regions, such as Invitrogen Stbl3™ or NEB Stable cells. This will ensure ...

  8. Fluorescence Titering Assay

    Type
    Protocol
    ...incubator Pipet controller Hazardous waste container Inverted fluorescence microscope Reagents DMEM high glucose...mL of DMEM complete. Mix well by pipetting or inverting. Aliquot 2 mL of cell suspension into each well...

  10. Kit Free RNA Extraction

    Type
    Protocol
    ... thoroughly by inversion. Add 1 mL water-saturated phenol, mix thoroughly by inversion. Add 0.2 mL of ...

  13. Water Bath Protocol

    Type
    Protocol
    ...depending on the model of water bath, but will likely involve a dial or a digital display you can set to the ...

  14. Centrifugation

    Type
    Protocol
    ...centrifuge. Immediately stop the centrifuge and investigate the issue. When you are finished spinning your...

  16. Gibson Assembly Protocol

    Type
    Protocol
    ...gene. This trick can also enable replacement of "inverse PCR" reactions with a two-part Gibson reaction ...

  17. Colony Formation Titering Assay

    Type
    Protocol
    ... 10 μg/mL polybrene. Mix well by pipetting or inverting. Aliquot 1.35 mL of the cell suspension into each...

  20. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...reintroduction, inversion and re-inversion happen at the same probability. Continuous inversion of the DNA ...recombinase, which is named after its ability to invert or ‘flip DNA’, is derived from the yeast S. cerevisiae...Recombination between these sites can lead to excision, inversion, and translocation of DNA in a similar fashion... Mutated FRT and loxP sites in Flex Vectors – inversion only once All recombination events mediated by...target sites have been engineered to avoid this re-inversion issue. It became possible to design FLEx vectors...vectors (Flip-excision vectors) that only allow one inversion event when it was discovered that it is not the... FLEx vectors, the DNA sequence that should be inverted is flanked by two pairs of target sites that differ...
Showing: 41 - 60 of 532 results