We narrowed to 157 results for: itch

1. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

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   ...advance your career, promote career growth, and/or switch your field of work, both in academia and in industry...specialized so it might lend itself more readily to a switch. 4. Do you want more autonomy?  Before my master...

2. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

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   ...depolarization. They therefore function as a neuron “on switch.” Neuronal inhibition: Hyperpolarization Conversely...the cell. It therefore functions as a neuron “off switch.” Because of the differing peak absorption wavelengths...

3. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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   ...activates transcription with the flick of a light switch and shuts off just as quickly. The original AraC...bacteriograph was created using 160 individual images stitched together. Image from Romano et al., 2021 with ...

4. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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   ...zebrafish embryo over an 80 minute time course.  By stitching together a stable scaffold and HA-binding regions...protein dynamics in vivo. In the future, this stitching technique could be applied to other epitopes and...

5. Hot Plasmids - January 2023

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   ...articles. Think we missed something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps...Andrews NP,  et. al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced...

6. Plasmids 101: Inducible Promoters

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   ...we’ll discuss inducible promoters, which can be switched from an OFF to an ON state, and how you might ...The Promoter Region Plasmids 101: Cre-lox Light-Switchable Two-Component Systems for E. coli Browse other...

7. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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   ...everything is conceptual, and the team that packages its pitch the best, wins. He explained that entrepreneurs ...the team works well, the idea is solid, and “the pitch is packaged to perfection”, seed money will hopefully...

8. Plasmid Tools for Microbiome Studies

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   ...At the heart of their method is the RNA toehold switch, a RNA hairpin that can be designed to bind and...interest is a benefit of this method. Find the toehold switch microbiota/biomarker sensors here Studying the ...

9. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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   ...threshold like a switch that turns on or off. CRISPR shows a more continuous response - not switch-like at all...

10. Plasmids 101: Cre-lox

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    ...Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre–...

11. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    ...lab at the university that I went to. Making that switch from being a grad student to an employee, and being...life changed––she had a daughter. Needing a career switch because of the demanding hours in the lab, she ...

12. Viral Vectors 101: Calcium Sensors

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    ...FRET sensors, can reduce these unwanted variables. Twitch is a FRET-based calcium reporter derived from troponin...sensor for neuroscientists is CaMPARI. This sensor switches from green fluorescence to a red fluorescent state...

13. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    ...including many things people already use. It makes the switch to Golden Gate Cloning easier because individual...with Golden Gate MoClo. The barrier for those switching to Golden Gate is a conceptual barrier. Sometimes...

14. Which Fluorescent Protein Should I Use?

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    ...Photoactivatable / Photoconvertible: These proteins can switch their color when activated by a specific excitation...the mEOS proteins. Some proteins are reversibly switchable (e.g. rsEGFP, Dreiklang). Fluorescent Timers ...

15. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    ...transcriptors,” transistor-like digital genetic switches that enable cellular computing. These logic gates...fluorescence. There are a few examples of engineered RNA switches, but we've not yet seen the development of enough...

16. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    ..., compromising the photon budget. Likewise, the switch from ensemble imaging to single-molecule imaging...best method for live cell labeling and the easiest switch from fluorescent proteins given the relative simplicity...

17. Hot Plasmids - November 2023

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    ...cultured rat hippocampal neurons. HaloTag dyes are switched from yellow to red at 0 h and back to red at 12...N.P., et al. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced...

18. Retrograde AAV viral preps

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    ...category by using the buttons. Controls Green Red Switch Other Recombinases Cre Flp VCre Dre Calcium Sensors...EF1a Nuc-flox(mCherry)-EGFP EF1a Color-flipping switch. Expresses NLS-mCherry in the absence of Cre, and...

19. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    ...therefore acts as a switch between the default RFP and CFP, while Brainbow 2.1 switches from the default ...

20. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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    ...important ease of use is for them (if they are to switch from paper to digital for example): “… 99% of respondents...retrievable, searchable and traceable. However, switching from paper to an electronic lab notebook requires...