pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
Type
Blog Post
...keratinocytes to peripheral blood mononuclear cells (PBMCs). PBMCs isolated from whole blood present many benefits...reprogramming while PBMCs collected from blood are reprogrammed immediately after collection [2].
PBMCs display unique...to derive iPSCs from PBMCs. Most laboratories use Sendai virus to reprogram PBMCs efficiently [5]. However...age-matched fibroblasts [3].
These advantages make PBMCs the ideal source of somatic cells for reprogramming... EBNA-1.
Methods for reprogramming hiPSCs from PBMCs
While the method of choice for reprogramming often...usage of Sendai virus.
Reprogramming efficiency of PBMCs with episomal vectors
Episomal vectors are based...reprogramming factors. However, reprogramming of PBMCs by episomal vectors has yielded hiPSCs at a low ...