We narrowed to 399 results for: nans

... Antibodies Addgene distributes ready-to-use recombinant antibodies made from plasmids in our collection...partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity Recommended Applications ... opens in a new window) NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection : The NeuroMab Facility...

...iRFP670 643 670 12.7 piRFP670-N1 miRFP670nano3 645 670 24 pcDNA-miRFP670nano3 miRFP680 661 680 14 pmiRFP680...

...KlacRS1 M. mazei L-lactyl-lysine (Klac) Bacterial TAG Nanxi Wang 232159 pEvol-chKlacRS-WT chKlacRS-WT M. mazei...M. barkeri L-lactyl-lysine (Klac) Bacterial TAG Nanxi Wang 232160 pEvol-chKlacRS-IPYE chKlacRS-IPYE M....M. barkeri L-lactyl-lysine (Klac) Bacterial TAG Nanxi Wang 240124 pIDTsmart-MbPylRS-4xPyOtR-ITR-GFP PylRS...

...to activate transcription, and they also have a dominant negative effect on functional p53 through oligomerization...Uncovering the role of p53 splice variants in human malignancy: a clinical perspective. Surget S, Khoury MP,...

...gene therapy development. In order to produce recombinant AAV vectors, researchers need to use three plasmids...cycle. To avoid the use of these viruses during recombinant AAV vector production, necessary helper virus...

...in Cynomolgus Macaques of rAAV2tYF-CB-hRS1, a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin...Sulfate Binding on Transduction of Retina by Recombinant Adeno-Associated Virus Vectors. J Virol . Mar...

...overall improved ease of use when expressing recombinant phosphoproteins. These attributes make rEcoli...& Jewett, M. C. (2015). Robust production of recombinant phosphoproteins using cell-free protein synthesis...

...break down how different contaminants impact these ratios.  Protein contaminants Whether you are purifying...microvolume spectrophotometers, the Thermo Scientific NanoDrop is one of the most popular options, so most of...of this post is based on reading results from a NanoDrop. The principles will apply to similar instruments...Characteristic absorption spectra for pure DNA and likely contaminants found in DNA samples. The blue lines in B-D ...B-D represent the spectra for the specified contaminant, while the grey lines in B-D represent the pure ...pure DNA spectra for comparison. Each of these contaminants will have a slightly different impact on the ...to varying degrees across the UV range used in NanoDrop systems. Peak absorption occurs below 230 nm, ...

...generate the nanomouse. (VHH = variable domains of heavy chain only antibodies) (B) Nanomice express hybrid...Antibodies  Secondary Nanobody Toolbox No Llamas Required - Synthetic Nanobodies Against Membrane Proteins...single-domain antibodies (sdAb), commercially known as nanobodies (Arbabi-Ghahroudi, 2017)!      Figure... single-domain antibody, under the commercial "Nanobody" name.  We’ve talked about sdAbs a number ...alpacas). But because sdAbs are so amenable to recombinant expression (and because maintaining a llama facility...mice that express camelid variable domains (aka nanomice), which gives researchers the “best of both worlds... Jin B, Odongo S, Radwanska M, Magez S (2023) Nanobodies: A Review of Generation, Diagnostics and Therapeutics...

...160374 Nanobodies- there's a simple method for that! By Michelle Cronin The benefits of nanobodies over ...antibodies. Nanobodies are easily expressed in the cytosol of E. coli, however the nanobody proteins tend...evolution Fluorescent tools for mammalian cells Nanobody purification CRISPRi and CRISPRa system for hPSCs...protease used to cleave affinity tags from purified recombinant proteins at small and large scale.  This new ... conventional antibodies can’t be overlooked. Nanobodies are smaller in size, more durable, and usually...alleviates the inclusion body problem and makes nanobody production in E.coli as simple as following a ...signal peptide of MBP, followed by the desired nanobody (NB) sequence, an AviTAG, and 6xHis tag. The MBP...

...Variables (scFvs). Nanobodies Figure 3: Comparison of the Hcab and nanobody. Nanobodies are the variable...antibodies here. Recombinant antibodies Similar to monoclonal antibodies, recombinant antibodies are a...synthetic nanobodies, or synbodies, can be produced from plasmids, saving time and money. Nanobodies and synbodies...from naturally-occurring immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences...to reach. While a full antibody is ~150 kDa, a nanobody is 12-15 kDa.   Research applications for antibodies...Collection includes plasmids that express antibodies, nanobodies, and ScFvs, as well as tools for developing and...Developmental Studies Hybridoma Bank Learn more about nanobodies Watch our Antibodies 101 animation, What is an...

...multicolor NIR imaging.  Small miRFPnano A separate class of NIR FPs, named miRFPnanos, were derived from cyanobacteriochrome...545 3.7 62 8 miRFP670nano3 645 670 129,000 18.5 387 Monomer 675 4.2 250 9 miRFP704nano 680 704 93,...to your camera.  Two-photon image of miRFP670nano3-expressing neurons in the neocortex of a living...Their advantages are their small size (17 kDa for miRFPnanos, 35 kDa for miRFPs, and 27 kDa for EGFP) and ...the proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular and molecular... 97 Monomer 510 4.5 117 12 CBCR-based FPs miRFP670nano https://www.addgene.org/127443/ 645 670 95,...93,000 9.9 149 Monomer 1265 4.1 81 miRFP718nano 690 718 79,000 5.6 72 Monomer 885 3.8 33 State-of-the-art...

...Plasmid Collection, including tools using NanoLuc®, NanoBiT, and more. Which luciferase and assay have...blog Luminescent Imaging with Nano-lanterns  Technologies Enabled by NanoLuc® Luciferase Fluorescent Proteins... engineered a variety of improved luciferases. NanoLuc® Luciferase is the best known: it’s derived from...gracilirostris) Natural 455 19 Coelenterazine None Yes NanoLuc®(derived from Oplophorus gracilirostris) Engineered... (fluorescent and luminescent) worlds Forster Resonance Energy Transfer (FRET) is the transfer of energy...which then emits it as light. Bioluminescence Resonance Energy Transfer (BRET) works the same way, except...protein-protein interactions Split luciferases, such as NanoBiT, are expressed in two subunits and only catalyze...

...microenvironment’s malignancy but also incite different organs to become malignant. Exosomes also contain...on the conversion of non-malignant forms of cancer to invasive and malignant forms and noticed that myoepithelial...that when there’s less caveolin 1, there’s less malignancy because there’s less proliferation. However, ...exosomes are tools of communication and provide malignancy to the microenvironment itself. They deliver ...myoepithelial cells prevented progression to malignancy, concluding that these cells may be considered...PMC4788391. Kogure, Takayuki, et al. "Intercellular nanovesicle‐mediated microRNA transfer: A mechanism of environmental...Central PMCID: PMC3310362. Martins, Diana, and Fernando Schmitt. "Microenvironment in breast tumorigenesis...

...through the use of protein tags and separated from contaminants using a variety of purification methods. Deciding...expression systems are very powerful for high level recombinant protein expression. These systems enable high...cheap and low-tech means of mass expression of recombinant proteins. Many cells from various types of plants...modifications. Extraction and purification of recombinant proteins from plants however can be expensive...biochemicals and proteins through plant roots. Tagging recombinant proteins with a naturally secreted plant peptide...expression system to generate large quantities of recombinant eukaryotic proteins. Although many species of...using S. cerevisiae, researchers often place recombinant proteins under the control of the galactose inducible...

...serotype of the packaged DNA and look for potential contaminants. The analysis is a 2-step process using Geneious...total reads are most likely to correspond to the contaminant. Each contig is then BLASTed against the NCBI...contaminated. For AAV-68544, the best match for the contaminant is Addgene plasmid #44362. Method validation,... were easily able to identify AAV-44632, the contaminant we mixed into the original AAV sample at 5%. ...software looks for and quantifies the presence of recombinants in recombinase-dependent sequences. Recombinase-dependent...powerful tool that can be used to identify DNA contaminants in AAV preparations and provide information ...Encapsidation of Prokaryotic Sequences during Recombinant Adeno-Associated Virus Production and Their in...

...to identify the critical NANOG binding sites: 1. Find Enhancers that have NANOG binding sites 2. Design...ChIP-seq experiment (think “GPS for proteins”) for NANOG, an essential transcription factor in embryonic ... just over 13,000 significant binding peaks for NANOG in the human genome. But not all of those 13,000...Design a CRISPR screen to target and disrupt the NANOG enhancers 3. CRISPR out the enhancers from step 2. ... ESCs die or differentiate Step 3 reveals which NANOG-related genes are critical to stem cell survival...CRISPR in the next section. In order to denote the NANOG enhancers from step 1 with genome math, we’ll need...shorthand from the field of epigenomics: H3 – one of NANOG’s associated histone proteins K4 and K27 – locations...

... technology, but Oxford Nanopore is one of the most popular options. Nanopore sequencing deviates from...3: Overview of Nanopore sequencing technology. Created with BioRender.com. Nanopore can provide longer...sequencing methods (Figure 3). It uses (aptly named) “nanopores” that are attached to the flow cell membrane and...proteins. When each nucleotide passes through the nanopore, the electrical current recorded by the instrument...-read sequencing. At Addgene, we routinely use Nanopore technology to sequence our packaged viral DNA ...an entire viral genome in one read. We also use Nanopore sequencing to QC plasmids that are highly repetitive... Y., Bollas, A., Wang, Y., & Au, K. F. (2021). Nanopore sequencing technology, bioinformatics and applications...

... GFP-binding nanobodies, the project really took off. Unlike most antibodies, nanobodies, which occur ...inside cells. Nanobodies binding GFP were designed in 2009. Subsequent work showed that nanobodies could be...and money needed to develop new lines. Using nanobodies to create GFP scaffolds for transcriptional activation...  References 1.Tang, Jonathan C.Y., et al. “A nanobody-based system using fluorescent proteins as scaffolds...Modulation of protein properties in living cells using nanobodies.” Nat. Struct. Mol. Biol. 17 (2010): 133–138....Fluorescent fusion protein knockout mediated by anti-GFP nanobody.” Nat. Struct. Mol. Biol. 19 (2012), 117–121. ...

...editing CHARM: A compact epigenetic silencer Recombinant antibodies for marking astrocytes MagIC-Cryo-EM... linkers and spacer proteins ending with a GFP nanobody (Figure 1). This enrichment streamlines the purification...alpha helix) and target capture module (SpyTag-GFP nanobody) assembled on beads. C) Micrograph of H1.8-GFP...spacers could enable many future variations using nanobodies or scFvs for other targets or could be used for...https://doi.org/10.1016/j.cell.2021.03.025.    Recombinant antibodies for marking cell types in the nervous...s Neuroscience Antibody Collection to find a recombinant anti-GFAP [N206A/8R] antibody shared by James...other cell types in the nervous system!  Find recombinant anti-GFAP [N206A/8R] antibody here!   ...