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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 388 results for: nans

Showing: 41 - 60 of 388 results

  2. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...overall improved ease of use when expressing recombinant phosphoproteins. These attributes make rEcoli...& Jewett, M. C. (2015). Robust production of recombinant phosphoproteins using cell-free protein synthesis...

  3. Resolute Plasmid Collection

    Type
    Collection
    ...partnership of 13 members from industry and academia financed by the Innovative Medicine Initiative (IMI) of...

  4. Mammalian RNAi Tools

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    Collection
    ...for cancer therapy . International Journal of Nanomedicine, 18 , 7605–7635. PubMed (Link opens in a new...

  5. Synthetic Biology - Overview

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    Collection
    ...Kit Optogenetics Pertz Lab cpFRET Kit Rinehart Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway...

  6. Viral Vector Guides and Plasmids

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    Collection
    ...–36 kb 105 nm Dividing/Non-dividing No High *Recombinant AAV has a low frequency of target cell genome...

  7. Microbiology Resources

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    Collection
    ...MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit design...

  8. Worm Expression Resources

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    Collection
    ...cycle (3 days from egg to adult) and easy lab maintenance. Worms are particularly well suited for studies...

  9. Fluorescent Protein Guide: FRET

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    Collection
    ... localization Optogenetics Background Förster resonance energy transfer, or FRET , is a process by which...

  10. Viral Production

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    Collection
    ...viral genome and the absence of unexpected DNA contaminants. The data is also used to determine recombination...

  11. CRISPR Guide

    Type
    Collection
    ...Cian, A., Itier, J. M., Anegon, I., Lopez, B., Giovannangeli, C., & Concordet, J. P. (2018). CtIP fusion ...prime editing systems by manipulating cellular determinants of editing outcomes. Cell . 184 (22), 5635-5652...

  12. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ...break down how different contaminants impact these ratios.  Protein contaminants Whether you are purifying...microvolume spectrophotometers, the Thermo Scientific NanoDrop is one of the most popular options, so most of...of this post is based on reading results from a NanoDrop. The principles will apply to similar instruments...Characteristic absorption spectra for pure DNA and likely contaminants found in DNA samples. The blue lines in B-D ...B-D represent the spectra for the specified contaminant, while the grey lines in B-D represent the pure ...pure DNA spectra for comparison. Each of these contaminants will have a slightly different impact on the ...to varying degrees across the UV range used in NanoDrop systems. Peak absorption occurs below 230 nm, ...

  13. Neurodegeneration Plasmid Collection

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    Collection
    ...Corporation 237172 EIF2AK2(252-551)-NanoLuc Fusion Vector EIF2AK2 NanoLuc CMV VWM disease Promega Corporation... Arrowsmith 211083 pNLF1-C_EIF2B2:1-351 EIF2B2 NanoLuc CMV VWM, CACH Cheryl Arrowsmith 211112 pBiT3.1-...Vector VCP Halo CMV ALS Promega Corporation 237148 NanoBRET Assay Vector, TREM2-HaloTag TREM2 Halo CMV Alzheimer's...

  14. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...generate the nanomouse. (VHH = variable domains of heavy chain only antibodies) (B) Nanomice express hybrid...Antibodies  Secondary Nanobody Toolbox No Llamas Required - Synthetic Nanobodies Against Membrane Proteins...single-domain antibodies (sdAb), commercially known as nanobodies (Arbabi-Ghahroudi, 2017)!      Figure... single-domain antibody, under the commercial "Nanobody" name.  We’ve talked about sdAbs a number ...alpacas). But because sdAbs are so amenable to recombinant expression (and because maintaining a llama facility...mice that express camelid variable domains (aka nanomice), which gives researchers the “best of both worlds... Jin B, Odongo S, Radwanska M, Magez S (2023) Nanobodies: A Review of Generation, Diagnostics and Therapeutics...

  15. Hot Plasmids - August 2020

    Type
    Blog Post
    ...160374 Nanobodies- there's a simple method for that! By Michelle Cronin The benefits of nanobodies over ...antibodies. Nanobodies are easily expressed in the cytosol of E. coli, however the nanobody proteins tend...evolution Fluorescent tools for mammalian cells Nanobody purification CRISPRi and CRISPRa system for hPSCs...protease used to cleave affinity tags from purified recombinant proteins at small and large scale.  This new ... conventional antibodies can’t be overlooked. Nanobodies are smaller in size, more durable, and usually...alleviates the inclusion body problem and makes nanobody production in E.coli as simple as following a ...signal peptide of MBP, followed by the desired nanobody (NB) sequence, an AviTAG, and 6xHis tag. The MBP...

  16. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...Variables (scFvs). Nanobodies Figure 3: Comparison of the Hcab and nanobody. Nanobodies are the variable...antibodies here. Recombinant antibodies Similar to monoclonal antibodies, recombinant antibodies are a...synthetic nanobodies, or synbodies, can be produced from plasmids, saving time and money. Nanobodies and synbodies...from naturally-occurring immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences...to reach. While a full antibody is ~150 kDa, a nanobody is 12-15 kDa.   Research applications for antibodies...Collection includes plasmids that express antibodies, nanobodies, and ScFvs, as well as tools for developing and...Developmental Studies Hybridoma Bank Learn more about nanobodies Watch our Antibodies 101 animation, What is an...

  17. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...multicolor NIR imaging.  Small miRFPnano A separate class of NIR FPs, named miRFPnanos, were derived from cyanobacteriochrome...545 3.7 62 8 miRFP670nano3 645 670 129,000 18.5 387 Monomer 675 4.2 250 9 miRFP704nano 680 704 93,...to your camera.  Two-photon image of miRFP670nano3-expressing neurons in the neocortex of a living...Their advantages are their small size (17 kDa for miRFPnanos, 35 kDa for miRFPs, and 27 kDa for EGFP) and ...the proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular and molecular... 97 Monomer 510 4.5 117 12 CBCR-based FPs miRFP670nano https://www.addgene.org/127443/ 645 670 95,...93,000 9.9 149 Monomer 1265 4.1 81 miRFP718nano 690 718 79,000 5.6 72 Monomer 885 3.8 33 State-of-the-art...

  18. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...Plasmid Collection, including tools using NanoLuc®, NanoBiT, and more. Which luciferase and assay have...blog Luminescent Imaging with Nano-lanterns  Technologies Enabled by NanoLuc® Luciferase Fluorescent Proteins... engineered a variety of improved luciferases. NanoLuc® Luciferase is the best known: it’s derived from...gracilirostris) Natural 455 19 Coelenterazine None Yes NanoLuc®(derived from Oplophorus gracilirostris) Engineered... (fluorescent and luminescent) worlds Forster Resonance Energy Transfer (FRET) is the transfer of energy...which then emits it as light. Bioluminescence Resonance Energy Transfer (BRET) works the same way, except...protein-protein interactions Split luciferases, such as NanoBiT, are expressed in two subunits and only catalyze...

  19. Immunology Research Plasmids and Resources

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    Collection
    ...few minutes of exposure to a pathogen and is the dominant defense strategy during the first several days...necrosis factor (TNF) family of proteins are predominantly expressed by immune cells and help to regulate...

  20. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...microenvironment’s malignancy but also incite different organs to become malignant. Exosomes also contain...on the conversion of non-malignant forms of cancer to invasive and malignant forms and noticed that myoepithelial...that when there’s less caveolin 1, there’s less malignancy because there’s less proliferation. However, ...exosomes are tools of communication and provide malignancy to the microenvironment itself. They deliver ...myoepithelial cells prevented progression to malignancy, concluding that these cells may be considered...PMC4788391. Kogure, Takayuki, et al. "Intercellular nanovesicle‐mediated microRNA transfer: A mechanism of environmental...Central PMCID: PMC3310362. Martins, Diana, and Fernando Schmitt. "Microenvironment in breast tumorigenesis...
Showing: 41 - 60 of 388 results