...Cloning High Complexity Golden Gate Cloning with NEB’s DAD New MoClo basics blog ...

... an endonuclease negative E. coli strain such as NEB stable. Dilute 1:3 (µg DNA:µg PEI) in 500 µL total...

...of unstable regions, such as Invitrogen Stbl3™ or NEB Stable cells. This will ensure that the repeats will...

... an endonuclease negative E. coli strain such as NEB stable. Make a mixture of a total of 500 μL PEI-OptiPro...

... companies, such as (Link opens in a new window) NEB , (Link opens in a new window) Qiagen , (Link opens...

...a linear piece (see (Link opens in a new window) NEB for more information). Thus, we recommend that you...

...Overview of NEBuilder HiFi DNA assembly method As with any well-executed cloning endeavor, NEBuilder requires...resource for planning an NEBuilder HiFi DNA Assembly mix reaction is the NEBuilder Assembly Tool which is...BioBrick® Assembly, Golden Gate Assembly, and NEBuilder® HiFi DNA Assembly. Gibson Assembly allows the...enzymes results in scarless assembly. Finally, the NEBuilder® HiFi DNA Assembly method from New England Biolabs...Plasmids 101 eBook! One of the features that makes NEBuilder HiFi so accurate (apart from the high-fidelity...PCR template. Some of the applications of the NEBuilder HiFi DNA Assembly Mix include:  Generation of...Generating constructs with multiple point mutations NEBuilder HiFi can simplify multi-position site-directed...

...selected for by manipulating the nebulization time and pressure. Nebulization units, which can be purchased...physical fragmentation strategy is preferable. Nebulization involves forcing DNA through a small opening...

...Mammalian Cell Lines pSpCas9(BB) (pX330) Link Parrott NEBuilder Assembly of CRISPR vectors using ssDNA oligos ...

...tonicity-responsive KIAA0827, NF-AT5, NFATL1, NFATZ, OREBP, TONEBP NFATC1 nuclear factor of activated T-cells, cytoplasmic...

...! #1millionplasmids On to the next million! @CarolineBME @AllostEricSite @LiannaSwanson etc. Missed you...

...University of Massachusetts, Worcester  University of Nebraska Medical Center University of Pennsylvania  University...

...iTOP (induced transduction by osmocytosis and propanebetaine) to describe this method of protein transduction...

... Dept. of Biochemistry and Biophysics and the Lineberger Comprehensive Cancer Center at UNC Chapel Hill...

... GuideRecombinant antibodies available from AddgeneBrowse our recombinant antibody plasmid collection ...

...What’s Your Story? By Herminia Ibarra and Kent Lineback Resources on the Addgene Blog Check Out Our ...