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We narrowed to 239 results for: p

Showing: 41 - 60 of 239 results

  1. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ...and other important information. Tip: A lowercase "p" is often used as the first letter of a plasmid name...and simply denotes that the object is a ‘plasmid’. “p” is for plasmid. pXXXXX-XXXX Step 1: Backbone name...

  2. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...which identifies a p value cut off. This method entails arranging all calculated p values from smallest...this by establishing a significance threshold at a p value less than or equal to the alpha value divided...the cut off for significance by finding the largest p value where its rank divided by the total number of...multiplied by the alpha value is less than or equal to the p value. There are drawbacks to these tests as they ...

  3. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...., Feliciano, P. R., Arif, A., Bohnuud, T., Fernandez, T. P., Gehrke, J. M., Grayson, P., Lee, K. D., ...updated by Jennifer Tsang in August 2020 and by Emily P. Bentley in September 2025. References and Resources...Grünewald, J., Zhou, R., Lareau, C. A., Garcia, S. P., Iyer, S., Miller, B. R., Langner, L. M., Hsu, J...., Levy, J. M., Tay, T., Newby, G. A., Maianti, J. P., Raguram, A., & Liu, D. R. (2018). Improving cytidine...McElroy, A. N., Pandey, S., Doman, J. L., Huang, T. P., Raguram, A., Banskota, S., Newby, G. A., Tolar, ...Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained genome targeting with near-...

  4. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... J, Glanville J, Wolf P, Frenzel A, Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018...doi.org/10.1080/19420862.2018.1445456  Holzlöhner P, Hanack K (2017) Generation of Murine Monoclonal Antibodies...

  5. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...Fotouhi, M., Bolivar, S. G., Ruiz Moleon, V., Eckmann, P., Worrall, D., McDowell, I., Southern, K., Reintsch...Bandrowski, A., Virk, H., Edwards, A. M., McPherson, P., & Laflamme, C. (2023). Scaling of an antibody validation...10.12688/f1000research.154034.1 Laflamme, C., McKeever, P. M., Kumar, R., Schwartz, J., Kolahdouzan, M., Chen... A. M., Healy, L. M., Robertson, J., & McPherson, P. S. (2019). Implementation of an antibody characterization...McCarthy, J., Amrani, Y., Duffy, S. M., Bradding, P., & Roach, K. M. (2021). TGFβ1 induces resistance ...Weston, C. A., Chachi, L., Roach, K. M., & Bradding, P. (2019). Validation of antibodies for the specific...

  6. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...recognizes a short, linear epitope derived from the P and V proteins of Simian virus 5 (SV5) (Randall et...doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski, P., & Randall, R. E. (1992). Construction of solid matrix-antibody-antigen...reporting of mechanical forces with PILATeS By Emily P. Bentley Time to get flexible! Alex Dunn’s lab and...plasmids here! Zhong, B. L., Elliot, J. M., Wang, P., Li, H., Hall, R. N., Wang, B., Prakash, M., & Dunn...

  7. An Introduction to Adenovirus

    Type
    Blog Post
    ...10.1016/B978-0-12-801238-3.02523-X. Hofmann, C., Löser, P., Cichon, G., Arnold, W., Both, G. W., & Strauss, ...doi.org/10.1136/jcp.s3-6.1.39. PMID: 4376152. Rowe, W. P., Huebner, R. J., Gilmore, L. K., Parrott, R. H., ...R. L., Garcia, K. Y., Goodrich, J. S., Lovett, S. P., Parker, K., Radune, D., Hatada, A., Pan, C. Y., ...Rizzo, K., Bertumen, J. B., Morales, C., Oluniyi, P. E., Nguyen, J., … Chiu, C. Y. (2023). Adeno-associated...

  8. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...References Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner...from this paper at Addgene. Amrani N, Gao XD, Liu P, Edraki A, Mir A, Ibraheim R, Gupta A, Sasaki KE, ...Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ (2018)...Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin...

  9. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...bloggers Roodolph (Roo) P. St Pierre and Rose C. St Pierre! Roodolph (Roo) P. St Pierre is currently ... was contributed by guest bloggers Roodolph (Roo) P. St Pierre and Rose C. St Pierre, founders of STEAMid...

  10. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...Y, Chik KK-H, Zhang X, Fung AY-F, Tsoi H-W, Cai J-P, Chan W-M, Ip JD, Chu AW-H, Zhou J, Lung DC, Kok K-H...Jia HP, Halabi C, Sigmund CD, Meyerholz DK, Kirby P, Look DC, Perlman S (2006) Lethal Infection of K18...doi.org/10.1128/jvi.02012-06 Ou X, Liu Y, Lei X, Li P, Mi D, Ren L, Guo L, Guo R, Chen T, Hu J, Xiang Z,...

  11. Bacterial Expression Systems

    Type
    Collection
    ..., gram-positive bacteria Gary Dunny 48095 pQF PQ5 p -isopropyl benzoate (cumate) Sphingomonas sp. , Caulobacter... smegmatis Matthias Wilmanns 44447 pLC290 pR/cmtO p -isopropyl benzoate (cumate) Methylobacterium extorquens...

  12. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Other Species Human Mouse Fly Yeast T. gondii E. coli P. aeruginosa S. pneumoniae M. tuberculosis M. smegmatis...aeruginosa PA14 234855 (Set 1) 1000000254 Inhibition P. aeruginosa X. Liu NA 1 5,981 (Set 1) 5,971 (Set 2...

  13. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ..., S., Amster, A., Arbuckle, J., Ayala, A., Baker, P. M., . . . Tasic, B. (2025). A suite of enhancer AAVs...., Fong, O., Yao, S., Mortrud, M., . . . Levi, B. P. (2021). Functional enhancer elements drive subclass-selective...

  14. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...N., Flad, K., Schmidt, M. F., Tabor, A., Gmeiner, P., Hübner, H., & Weikert, D. (2020). Fluorescent ligands...78827-9 Breen, C. J., Raverdeau, M., & Voorheis, H. P. (2016). Development of a quantitative fluorescence-based...W. C., Patel, R., Lu, R., Macklin, J. J., Keller, P. J., Ji, N., & Lavis, L. D. (2017). A general method...., Parisi, M., Cincotti, G., Rampioni, G., Visca, P., & Kolmakov, K. (2023). Expanding the microbiologist...Grondin, Y., Rogers, R., Bousfield, D., & Demokritou, P. (2019). Development & characterization of fluorescently...

  15. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Insert Promoter PI Publication 64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-1 Fujii Efficient isolation...

  16. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ....org/10.1177/002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding...In J. Sturtevant (Ed.), PLoS ONE (Vol. 7, Issue 6, p. e38313). Public Library of Science (PLoS). https:...

  17. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...References: Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR, Maguire A, Rex TS...https://doi.org/10.1038/nbt1153 Odom GL, Gregorevic P, Allen JM, Chamberlain JS (2011) Gene Therapy of mdx...://doi.org/10.1038/mt.2010.205 Trapani I, Colella P, Sommella A, Iodice C, Cesi G, Simone S, Marrocco ....201302948 Trapani I, Toriello E, de Simone S, Colella P, Iodice C, Polishchuk EV, Sommella A, Colecchi L, ...

  18. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... and Resources References Chen X, Choo H, Huang X-P, Yang X, Stone O, Roth BL, Jin J (2015) The First .../doi.org/10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez M, Hu X,...content/364/6436/eaav5282.long Redfern CH, Coward P, Degtyarev MY, Lee EK, Kwa AT, Hennighausen L, Bujard...

  19. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...resources References Chadeuf G, Ciron C, Moullier P, Salvetti A (2005) Evidence for Encapsidation of Prokaryotic...Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine M, Broucque F, Robin C, Hebben M,...Merten O-W, Blouin V, François A, Redon R, Moullier P, Léger A (2015) Advanced Characterization of DNA Molecules...

  20. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...colonies, but some bacteria, including E. faecalis and P. mirabilis, had only ~1 transfer per 1,000,000 colonies...human disease (S. aureus, Klebsiella pneumoniae, and P. aeruginosa) in addition to Vicrio casei, a bacteria...Enterobacter aerogenes, Klebsiella pneumoniae, and P. aeruginosa (all opportunistic pathogens) were grown...
Showing: 41 - 60 of 239 results