We narrowed to 883 results for: prot

... fluorescent protein database As recognized by the 2008 Nobel Prize, fluorescent proteins (FPs) have become...users can submit new fluorescent proteins, add data to existing proteins when missing, or edit inaccuracies... Table of Fluorescent Protein Properties provides a master list of all proteins in the database, and the...Every protein has a page with all the information in the database relating to that specific protein (for...EGFP).  Users can “favorite” certain proteins, or create custom protein collections (such as a set of FPs...Choosing the B(right)est Fluorescent Protein Choosing Your Fluorescent Proteins for Multicolor Imaging Resources...fluorescent protein plasmids Find plasmids for FRET Check out the Michael Davidson Fluorescent Protein Collection...

...sybodies in their coat protein to screen for binding to the target protein. The protocol describes going through...small (12-15 kDa) single-domain proteins that can bind effectively to protein targets, similar to how antibodies... sybodies against membrane proteins Developing antibodies against proteins embedded in membranes is tricky...abundant than some other proteins. The Seeger lab developed a robust in-vitro protocol that enables rapid selection...target protein. The Seeger lab designed 3 different mRNA sybody libraries for use in the protocol, which...biotinylated (for purification purposes) protein target of interest, the protocol goes through 3 major steps to ...to the protein of interest. The Seeger lab used the ELISA technique described in their protocol because...

...connected to a TEV protease cleavage site. TEV protease is a highly specific protease that is commonly ...activate a cascade of caspases, protease enzymes that cleave proteins. Executioner caspases are activated...commonly used to cleave proteins. This protease reporter based on sfCherry however did not initially fluoresce...References Cabantous, Stéphanie, et al. "A new protein-protein interaction sensor based on tripartite split-GFP...blog Read our fluorescent proteins blog posts Download the fluorescent proteins 101 eBook Read our plasmids...Browse the fluorescent protein collection Find empty backbones for fluorescent protein fusions Browse the ...fluorescence changes of the donor and acceptor after proteolytic cleavage. Secondly, fluorescence imaging of living...

...about these proteins is that they are monomeric fluorescent proteins. Fluorescent proteins that oligomerize...plasmids containing the fluorescent protein mNeonGreen. This fluorescent protein joins mTFP1 and mWasabi, as ...yellow-green fluorescent protein mNeonGreen is a yellow-green fluorescent protein that was derived from ...fluorescent protein wavelengths, it can be imaged either with standard green fluorescent protein filters ...fluorescent proteins. Find mNeonGreen plasmids here! mTFP1: a monomeric teal fluorescent protein mTPF1 is...tetrameric cyan fluorescent protein from the coral Clavularia sp. protein CFP484. Hui-wang Ai and colleagues...fluorescent protein The team that developed mTFP1 further sought to identify why this protein was blue-shifted...

...binding proteins waiting to be explored and exploited. With better characterization, these proteins could...polymerase and a few proteases knocked out making them particularly useful for protein production), then ...forceps. Several protein induction conditions may need to be tested, as with any protein purification, but...same location as a given protein, you have a potential receptor binding protein hit and can go back to ...of the recombinant proteins. Optimal salt concentration will vary with each protein, but several of the...Purposes Use Yeast Two Hybrid Systems to Probe Protein-Protein Interactions Use CRISPR For Genome-Wide Screens...their bacterial prey. Currently, receptor binding proteins (RBPs, the host recognition factors of phages)...

...track down every last one of those gene regulatory proteins and the places in the genome where they can be...ve recently adapted the CRISPR/Cas9 system for protein tagging. We spoke with Mendenhall and Myers about...that are currently available along with detailed protocols, go to www.addgene.org/crispr/tagging. Addgene... What motivated you to adapt CRISPR for use in protein tagging? Myers: Our labs work really closely together...way, you can actually use this for tagging any protein in all kinds of different assays, not just transcription...system allows you to add this same antibody to any protein to get really beautiful ChIP-Seq results. The FLAG...t think it interferes with the function of the protein. Myers: We’ve done comparisons of our new system...

... do so with fluorescent protein labels requires multiple fluorescent proteins whose excitation and emission...proliferation of fluorescent proteins in recent years, there are many fluorescent protein combinations that can...fluorescent proteins requires some thought. The first step in choosing fluorescent proteins for your multi-color...aware of what fluorescent proteins are available. With new fluorescent proteins being published every month...fluorescent proteins, I maintain an interactive graph and table of the best fluorescent proteins currently...Choosing compatible fluorescent proteins To choose a set of fluorescent proteins to be imaged together, you...between the two fluorescent proteins. If you must image fluorescent proteins whose spectra overlap, there...

...GFP can exist in a protonated and a deprotonated state (Tsien, 1998). The deprotonated state has an absorbance...for another protein. Effectively, it equips the protein of interest with an additional protein module that...naturally occurring co-factor binding proteins, systems wherein a protein binds a synthetic, fluorescent analogue...available green fluorescent proteins emit light. The pKa of fluorescent proteins can be accurately determined...the localization of the fusion protein. Also, in cases where the protein of interest is relatively small...2017). GFP can only be used for tagging proteins GFP fusion proteins are made by connecting its DNA code ...bright red fluorescent proteins (e.g. mScarlet) or infrared fluorescent proteins (Chernov et al., 2017)...

...antibody against the protein of interest. The Protein A fused MNase is then added and Protein A binds Immunoglobulin...targeting MNase to antibody bound proteins (Figure 1). Importantly, since Protein A has weaker affinity for mouse... use FRET to study protein interactions Learn about other types of fusion proteins Study chromatin interactions...ChIP-Seq, is the go-to method for mapping where a protein binds genome-wide, and has been widely applied ...Although ChIP-seq is a relatively simple and robust protocol it does have limitations. The enzyme-based CUT...limitations and makes it easier for you to map protein-DNA interaction with limited biological materials...initial fixation step. This serves to stabilize DNA-protein interactions for subsequent sheering of DNA by ...

...Overview of Affinity Tags for Protein Purification. Current Protocols in Protein Science 73: . https://doi.org...immunohistochemistry methods, these proteins are routinely fused to ‘tag’ or ‘label’ proteins to facilitate its detection... red fluorescent proteins) allow simultaneous identification of more than one protein of interest. However... that fluorescent proteins might not always be the best option. Fluorescent proteins can interfere with...with fused protein function Under some circumstances, the fused fluorescent proteins can be a roadblock... in the expression of the protein of interest. The green fluorescent protein (GFP) and mCherry, two of...trafficking of the fusion protein (Kimple et al., 2013). We found that the fluorescent proteins can, indeed, interfere...

...integral membrane proteins and membrane associated proteins (i.e. GPI-anchored proteins), which are more... Post on Protein Tags Read Other Posts on Fluorescent Proteins Browse Our Fluorescent Protein Pages Find...of fusion protein levels. It remains unclear whether the FP misfolding impacts fusion protein function ... of fusion protein activity. We encourage investigators to characterize FP-fusion proteins functionally...the untagged protein of interest. To avoid the accumulation of dark fluorescent proteins in your experiments...and use of fluorescent fusion proteins in cell biology."Current Protocols in Cell Biology (2005): 21-4....to many microscopy experiments with fluorescent proteins (FPs) localized to compartments other than the...

...finally characterization. Read and download the full protocol from the JOVE article here on Addgene's website...

... tagged with mEmerald. Plasmids to monitor protein-protein interactions through multicolor labeling and...from Joachim Goedhart: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection.  mEmerald...Updated May 10, 2021. In the world of fluorescent proteins and their use for imaging cell biology, Michael...format on Addgene’s Michael Davidson Fluorescent Protein Collection webpage. These plasmids are divided...plasmid backbones spanning a rainbow of fluorescent proteins “It’s a great collection with over 300 backbones...impressively big deposit. “He has every fluorescent protein under the sun, from the standard oldies but goodies...interest isn’t in the ORF collection, the fluorescent proteins are, which makes mixing and matching a relatively...

...approaches to study protein interactions is the pull-down assay, wherein a protein of interest can be ...IP/MS, a target protein is first immunoprecipitated - along with its associated protein complexes - from...using antibodies against the target protein. The captured protein complexes are then analysed by mass ...interacting proteins. Similarly, in ChIP-seq assays, chromatin fragments that are bound by a protein of interest...target protein. Both IP/MS and ChIP-seq methods provide a wealth of information about how protein networks...pre-validated antibody for each target protein. To study 20,000 human proteins, this would be time-consuming, ...tag the protein of interest with a small biochemical “epitope tag” such that these tagged proteins can be...

... of the fluorescent proteins from the injected construct. The fluorescent protein expressed in a single... zebrafish." Cold Spring Harbor Protocols 2011.1 (2011): pdb-prot5546. Richardson, Alexander, et al. "...fluorescent proteins topic page Resources on Addgene.org Browse Addgene’s fluorescent protein collection...of a transgene construct containing fluorescent proteins (XFP) of different colors to label an organ or... label neurons. Genes encoding the fluorescent proteins are organized in tandem with a single promoter..., stability, and brightness of the fluorescent proteins used in the system. One such system is Brainbow...activate the expression of a different fluorescent protein. Therefore, each cell could express a unique combination...

...choose your own protein purification adventure. You want to purify your favorite protein (YFP). You have...bind and release a protein of interest in response to light allows for tagless protein purification as well...the core of the protein. OptoNBs were also tagged with an infrared fluorescent protein tag which let the...purification of unmodified proteins. Affinity purification methods typically require a protein to be tagged, which...approaches to use with recombinant proteins. Purifying an untagged protein with a flip of a light switch would...to light-controlled protein binding, they probably don’t bind your favorite protein yet. More OptoNBs would... you wash several times to remove non-specific proteins, and then elute YFP.  Option #2: Opto-Nanobodies...

...system is likely applicable to many types of proteins. For proteins with a modular structure, constructing ...Other proteins may also be utliized as scaffolds, including the set of red fluorescent proteins. The T-DOGGs...Cre-lox Resource Guide Fluorescent Protein Resource Guide Fluorescent Protein Blog Posts ...depositors find new ways to make this workhorse protein even more useful! From FPs optimized for oxidizing...researcher Jonathan Tang wanted to express a variety of proteins in single cell types in the mouse, but knew it...showed that nanobodies could be fused to other proteins, and these fusions retained the ability to bind... Tang et al. tested pairs of their GFP-binding proteins (GBPs) to find those that could co-occupy GFP....

...basic-helix-loop-helix) protein. After fusing the CRY2 and CIB1 domains to separate proteins of interest, a researcher... oligomerization when fused to a protein of interest. Fusion protein oligomerization happens within seconds...photoactivatable fluorescent protein Dronpa. The authors tracked protein kinase A (PKA) activity at the...phosphatase activity, second messenger activity, and protein-protein interactions. We’re excited to see how you ...yellow fluorescent protein (YFP) with an excitation peak of 519 nm. These proteins have improved photophysical...robust and efficient oligomerization of target proteins in response to blue light. This tool, CRY2clust...also be used to control homo-oligomerization of a protein fused to CRY2, although some studies have shown...

...lacked an “off-switch” for Cas proteins. Discovery of anti-CRISPR proteins That is, until the discovery...of anti-CRISPR proteins in 2012 (Bondy-Denomy et al., 2012). Anti-CRISPR (Acr) proteins are phage-derived...Marino et al., 2020). Some Acr proteins are specific to a particular Cas protein while others can inhibit CRISPR...fourth Acr protein discovered that blocks type II-A CRISPR-Cas systems. A full list of Acr proteins organized... found here. How do anti-CRISPR proteins work? Anti-CRISPRs proteins are highly diverse but most block... be as effective. This is where Acr proteins come in. Acr proteins are small enough that they could be...technology is constantly evolving. Variants of Cas proteins can be used for genome editing, activating gene...

...Institute focuses on proteomics: developing and applying new technologies to quantify proteins in tissues, cells...locus and labels the proteins in its vicinity with biotin. The biotin-labeled proteins can then be isolated...investigated protein binding at the TERT gene, but this method enables the unbiased discovery of proteins involved... anti-GFP nanobody is able to bind fluorescent proteins derived from A. victoria the functionalized nanobodies...nanobodies described here can be used to target any protein fused with GFP, YFP, mCerulean, or other related...: 29915061.        A new method to identify proteins occupying a specific genomic locus: Caspex  Article...group, developed a CRISPR-based approach to detect proteins that associate with specific genomic loci. He ...