We narrowed to 129 results for: sacs

1. Optogenetics + CRISPR, Using Light to Control Genome Editing

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   Blog Post

   ...photoswitchable spCas9, dCas9, and S. aureus Cas9 (saCas9) proteins, showing the versatility of this approach...This study generated the first optically active saCas9 protein. Stop light! Photoactive anti-CRISPRs ...

2. 15 Hot Plasmids from 2017

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   ...Features 85169  pJL-SaBE3  SaCas9 (NNGRRT)    85170  pJL-SaKKH-BE3  SaCas9 (NNGRRT)    85171  pBK-VQR-BE3...

3. CRISPR Guide

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   ...enzyme, additional Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence...meningitidis ), which are about the same size as SaCas9, with higher fidelity than SpCas9. FrCas9 (from...GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus sp. (AsCpf1...

4. Trimmer Lab NeuroMab Collection

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   ...receptor Rat Mouse IgG2a 188151 Anti-Fig4/Sac3 [N202/7R] Fig4/Sac3 Mouse Mouse IgG2a 188153 Anti-Cav1.2 Ca2...Chapsyn-110 Rat Mouse IgG2a 220408 Fig4/Sac3 [N202/38R] Fig4/Sac3 Mouse Mouse IgG2a 220409 TRIP8b (constant...

5. Fluorescent Protein Guide: Biosensors

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   ...PLoS One. 2014 Jun 5;9(6):e99095. Joseph Santos-Sacchi Chloride (Cl-) ClopHensorN chloride sensor (cytosolic...sensors for quantitative monitoring of pentose and disaccharide accumulation in bacteria. Biotechnol Biofuels...

6. Addgene's 20th Anniversary Party

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   Blog Post

   ...travel to Addgene's headquarters in Watertown, Massachusetts, for the occasion! Addgenies enjoying the ...

7. New Podcast Segment: Hot Plasmids

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   Blog Post

   ...this kit to complement unused auxotrophies in Saccharomyces strains.  Listen to Our Segment on The Yeast...

8. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

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   Blog Post

   ...Alex Chavez, a Clinical Fellow in Pathology at Masachusetts General Hospital and Postdoctoral Fellow in ...

9. #DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La

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   Blog Post

   ...finally December, and here at Addgene HQ in Massachusetts that means it’s time for near-freezing temperatures...

10. 2018 #DeckTheLab Contest Winners

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    Blog Post

    ... chilly this winter as the Addgenies are in Massachusetts, have a look at the Dyer Lab’s decorated office...

11. AAV Viral Preps

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    Collection

    ...Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation, CRISPR...

12. Plasmid Preps: Different Purity, Different Quantities, Different Uses

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    Blog Post

    ...be entirely endotoxin free. Endotoxin is a lipopolysaccharide of the cell wall of Gram negative bacteria...

13. Making Career Development an Integral Part of the Science Training System

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    Blog Post

    ...spearheaded by Cynthia Fuhrmann at University of Massachusetts Medical School. It has been supported by a Steering...

14. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

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    Blog Post

    ...Human Rights Commission and mediator for the Massachusetts small claims court summary process. Julie is...

15. Bringing Sustainable Practices to the Lab: Innovations

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    Blog Post

    ... that are better for the environment without sacrificing performance or quality. To do this, we can use...

16. Editor's Choice, September 2016

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    Blog Post

    ...Proteins," was contributed by guest blogger Jessica Sacher from the University of Alberta and describes a ...

17. Our Most Popular Blog Posts from 2019

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    Blog Post

    ...negative selection (ex: toxin-antitoxin systems and sacB counter-selection) tools used in cloning. 8. Bright...

18. What's New in CRISPR - March 2020

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    Blog Post

    ...that controls repression of gene expression in Saccharomyces cerevisiae. The system consists of dCas9, the...

19. The Stingy Scientist: How the Baby Gel Box Was Born

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    Blog Post

    ...recycle our discards according to the Cambridge, Massachusetts guidelines and wear an extra sweater so we don...

20. The Golden Stopwatch Award: Recognizing Facilitators of Research

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    Blog Post

    ...University of California, Irvine University of Massachusetts, Worcester  University of Nebraska Medical Center...