...cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast. 2004 Jun;21(8):661-70. doi...antibiotic selection markers for engineering in Saccharomyces cerevisiae. Microb Cell Fact. 2013 Oct 25;12...

...application and by color. External Resources European Saccharomyces Cerevisiae Archive for Functional Analysis (...Reagent Program (Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window...

...travel to Addgene's headquarters in Watertown, Massachusetts, for the occasion! Addgenies enjoying the ...

...this kit to complement unused auxotrophies in Saccharomyces strains.  Listen to Our Segment on The Yeast...

...Alex Chavez, a Clinical Fellow in Pathology at Masachusetts General Hospital and Postdoctoral Fellow in ...

...finally December, and here at Addgene HQ in Massachusetts that means it’s time for near-freezing temperatures...

... chilly this winter as the Addgenies are in Massachusetts, have a look at the Dyer Lab’s decorated office...

...be entirely endotoxin free. Endotoxin is a lipopolysaccharide of the cell wall of Gram negative bacteria...

...spearheaded by Cynthia Fuhrmann at University of Massachusetts Medical School. It has been supported by a Steering...

...Human Rights Commission and mediator for the Massachusetts small claims court summary process. Julie is...

...Proteins," was contributed by guest blogger Jessica Sacher from the University of Alberta and describes a ...

... that are better for the environment without sacrificing performance or quality. To do this, we can use...

...negative selection (ex: toxin-antitoxin systems and sacB counter-selection) tools used in cloning. 8. Bright...

...enzyme, additional Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence...meningitidis ), which are about the same size as SaCas9, with higher fidelity than SpCas9. FrCas9 (from...GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus sp. (AsCpf1...

...receptor Rat Mouse IgG2a 188151 Anti-Fig4/Sac3 [N202/7R] Fig4/Sac3 Mouse Mouse IgG2a 188153 Anti-Cav1.2 Ca2...Chapsyn-110 Rat Mouse IgG2a 220408 Fig4/Sac3 [N202/38R] Fig4/Sac3 Mouse Mouse IgG2a 220409 TRIP8b (constant...

...that controls repression of gene expression in Saccharomyces cerevisiae. The system consists of dCas9, the...

...recycle our discards according to the Cambridge, Massachusetts guidelines and wear an extra sweater so we don...

...Sontheimer and his group from the University of Massachusetts Medical School have developed a group of plasmids...

...University of California, Irvine University of Massachusetts, Worcester  University of Nebraska Medical Center...

...like GFP, some smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of interest. Promoter...