...reconstructed a transposase system based on the naturally mutated and non-functional transposase gene found...expression to study gene function. Because this transposase system was “awakened from a long evolutionary...

...along with a helper plasmid that expresses the transposase. The donor plasmid contains the gene(s) of interest...flanked by terminal repeat sequences, which the PB transposase recognizes. Following reprogramming, transgenes...transgenes can be removed by re-expressing the transposase. A challenge of using PB to generate iPSCs is that...

...exchanges bound GDP with GTP, resulting in the disassociation of the Gα subunit from the Gβγ dimer, activating... in response to the ligand, the complex will disassociate, resulting in a decreased GFP2 emission. The...

...exchanges bound GDP with GTP, resulting in the disassociation of the Gα subunit from the Gβγ dimer. The Gα...References and resources References Kroeze WK, Sassano MF, Huang X-P, Lansu K, McCorvy JD, Giguère PM,...

...protein (HRP), primarily used in Western blots and ELISAs, cannot be stored in sodium azide at all, as it...of signaling molecules. Assay Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that...

...lends itself well to assays like western blots and ELISAs. Chromogenic reactions produce a color change, ...immunohistochemistry, though they are also a common choice for ELISAs.  Biotin Biotin is used in a range of antibody-...

...cytometry’s standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry ...Introduction to Immunofluorescence Antibodies 101: ELISAs Antibodies 101: Introduction to Antibodies Resources...

...Types of ELISA There are four different types of ELISAs, which differ in the way the antigen is detected...available for your antigen as the other types of ELISAs require two different antibodies. As mentioned ...

... 137864 pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSAS) iCre-ERT2 (PAGSAS N-terminus) - Tamoxifen inducible CAG ...

...bypassed by leaving out Cas3 — a tactic some CRISPR transposase systems utilize with type I-F Cascades. ...Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S., Yamamoto, T., Sano...

...cotransfecting them with a plasmid expressing PiggyBac transposase. In rtTA-cell lines, it is better to first engineer...the mARG cassettes 2:1 (A:N-V) with PiggyBac transposase and outgrow the cells for a several generations...

...new optogenetic tool for localized microtubule disassembly, Opto-katanin (Meiring et al., 2022), that overcomes... optogenetic tool for localized, microtubule disassembly. Current Biology, 32(21), 4660-4674.e6. https...

.... Type I systems are also employed as CRISPR transposases, by leaving out Cas3. Type I is further subdivided...paradoxically called V-K, is a CRISPR-associated transposase (CAST) that can insert large fragments of DNA...

...in the experimental samples. Get more details on ELISAs in our article Antibodies 101: ELISA (Enzyme-linked...in a series of samples, such as Western blots or ELISAs, or for other experiments to study protein-protein...

...generates large genomic deletions. Insert NEW CRISPR transposases insert large fragments of DNA. Transcriptional...

...Use a CRISPRi system to target alpha-synuclein. Sastre et al. Sci Rep. 2023 Oct 18. Target neural oxytocin...

...TGCCAGCACAATAGATTGcaatggcaccgggttgGCAAGAGATCGCGTCGCA musashi-2a TAL3122 & TAL3123 TGGAAGCGGACGCGAGTCaagtcacctctggaagCCTGAACGATTCGCAACA...

...shows how antibodies are used to study proteins via ELISAs, western blots, immunoprecipitation, immunohistochemistry...

...CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3 system. We also describe...

...Cytology group! @EikeMahlandt @AnoeskaMoosdijk @Saskia_deMan @SILS_UvA @addgene #SciArt #fluorescence #LCAM...