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We narrowed to 253 results for: sms

Showing: 41 - 60 of 253 results

  1. Plasmids 101: The Wide World of Natural Plasmids

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    Blog Post
    ... a variety of functions that allow their host organisms to thrive in competitive environments. The genes...encode genes that allow the host to infect other organisms. If you’ve ever wondered why E. coli is safe to...means to deliver DNA and other molecules to host organisms. For instance, vectors derived from the Agrobacterium...like by living where no one else wants to. Some organisms manage this with plasmids that allow them to eat... eat unusual substrates that other organisms can’t use. Other plasmids offer resistance to toxic substances... which protect the host from attacks by other organisms. To conserve energy, these specialized degradative...

  2. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...to one or a subset of organisms. Examples: “organism agnostic”, “model organisms”, “small genome”, “large...that is applicable to only one, or a subset of organisms. In only a few cases the software is organism ... programming language or another. Supported organisms: This section lists the genomes supported by each...organism agnostic and have currently available organisms listed in this section. CRISPR software advice... were developed or by their focus on specific organisms. For this reason I generally recommend that laboratories...purpose of applying CRISPR technologies to new organisms and will help you whether you have a full assembly...

  3. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...for ATP to re-activate DnaA. These regulatory mechanisms allow E. coli to control chromosomal replication..., it can be regulated indirectly via the same mechanisms that affect DnaA and IHF. RepA also controls ...needs to get before handcuffing occurs. These mechanisms to regulate plasmid replication through the dual...Figure 3: Comparison of replication regulation mechanisms of stringently controlled plasmids pSC101 and... or sequences necessary for initiation or the mechanisms that regulate their replication, but their copy...Espíndola-Serna, L., & Martínez-Antonio, A. (2011). Mechanisms and Controls of DNA Replication in Bacteria. ...

  4. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...many organisms. When supplemented with adenine analogs such as 2-fluoroadenine (2-FA), organisms with ...Fortunately, researchers studying plants and other organisms lacking this innate immunity need not worry and... of APT and any gene target of interest, only organisms that are actively silencing to a sufficient degree...University of Zurich. He is fascinated by how organisms exquisitely and robustly self-organize at the ....186452 Wilson RC, Doudna JA (2013) Molecular Mechanisms of RNA Interference. Annu Rev Biophys 42:217–...

  5. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post
    ...across organisms. When using the MK system, many of the parts can be used in a variety of organisms, saving...MoClo kit recommendations for different modal organisms and applications.    Plant applications We...libraries and tools available for certain model organisms and backgrounds, MoClo can be invaluable for more... complex editing and screening experiments in organisms that don’t yet have these tools built out. While...many MoClo kits are designed with specific model organisms in mind, others are designed for flexibility. ...

  7. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...the genetic code is universal, many different organisms actually prefer certain codons over others for...for codon-usage of 257,468 genes across 8,792 organisms (Nakamura et al., 2000).  What is codon optimization...mutations throughout a gene of interest based on an organisms codon usage bias to increase translational efficiency...codons. (B) Example of codon usage among different organisms. There are several codons that encode the same...scientists codon optimize genes from eukaryotic organisms for expression in prokaryotic systems or yeast...

  8. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ... ease of use and utility in a wide variety of organisms.  While much of current CRISPR research focuses... plant species, including commonly used model organisms like Arabidopsis and Medicago truncatula and several...universal functionality of the CRISPR system in most organisms, some plant-specific changes to CRISPR components...experimental design principles developed in other model organisms (see our CRISPR guide for common CRISPR principles...sequence) is consistent between plants and other organisms, but the promoter used to drive gRNA expression...

  9. Using AAV for Neuronal Tracing

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    Blog Post
    ...transport suggest that there are several parallel mechanisms in place (for review see Maday et al., 2014)....are capable of synaptic spread. The underlying mechanisms of trans-synaptic trafficking are not fully understood...these two modes of transport may share common mechanisms. Rare observations like these may be a starting...brain. A better understanding of the routes and mechanisms of intracellular transport of AAV could facilitate...Adeno-associated virus serotypes 1, 8 and 9 share conserved mechanisms for anterograde and retrograde axonal transport...E.L.F. (2014). Axonal Transport: Cargo-Specific Mechanisms of Motility and Regulation. Neuron 84(2), 292...

  10. Fluorescent Proteins 101: History of Fluorescent Proteins

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    Blog Post
    ...These molecules occur naturally in bioluminescent organisms, but their real power lies in the clever ways ...Although humans have known about bioluminescent organisms and their characteristic radiance for some time...collaborator Martin Chalfie expressed GFP in exogenous organisms (E. coli, and later C. elegans) for the first ...be responsible and began studying these marine organisms to find the culprits. He even obtained samples...

  11. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    ...this lies in the codon usage biases of different organisms. Different species have different preferences ...not much variation in GC content between many organisms and DNA synthesis companies often work within ...trend of sourcing genes from distantly related organisms. In 2006, less than 1% of the genes deposited ...accidental or intentional release of engineered organisms. “Is [the organism] engineered? Where did it come...

  12. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...host-defense mechanisms to enable multiplex gene editing in a variety of cells and organisms. However, lengthy...processing mechanism, which is conserved in all living organisms and has previously been used for multiplex Cas9...nuclease editing, in virtually any cell type or organisms of interest with only minimal genetic payloads...

  13. K. phaffii: Rising to the Occasion

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    Blog Post
    ...model organism Yeast are single cell, eukaryotic organisms formally classified as fungi. They reproduce by... methanol metabolism occurs in methylotrophic organisms. K. phaffii is an excellent model for peroxisome...et al., Komagataella phaffi as Emerging Model Organisms in Fundamental Research. Front. Microbiol. 11-...Resources on the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening...

  14. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...research projects.   Understanding drug resistance mechanisms of Mycobacterium tuberculosis Dr. Patricia Sheen...her group are interested in understanding the mechanisms behind Mycobacterium tuberculosis resistance ... investigates the molecular and cellular etiopathomechanisms of ischemic stroke, the most common type ...extreme life on earth including extremophile microorganisms, biomarkers, and recently, cancer research ...

  15. Viral Vectors 101: Viruses as Biological Tools

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    Blog Post
    ...easily deliver their “cargo” to target cells and organisms, and when that cargo is designed to make you sick...biological tools In nature, viruses infect cells and organisms (which we refer to as hosts) via viral particles...and fuse with. In nature, this determines which organisms and cells they infect. In the lab, this means ... makes them ideal for applications with whole organisms. Finally, viral delivery is typically straightforward...

  16. All in a Twist: dsRNA

    Type
    Blog Post
    ...knew about all the diverse in vitro production mechanisms. siRNA, also known as RNAi, are short, dsRNA ...previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources of dsRNA ...response. However, if dsRNA sensors are mutated or mechanisms which suppress endogenous dsRNA formation are...levels, possibly due to dysregulation of removal mechanisms, an array of diseases can result (Fanconi Anemia...

  17. Plasmids 101: Gateway Cloning

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    Blog Post
    ...variety of model organisms. Use the links below to find Gateway plasmids for your organisms of interest: ... Genomic DNA and is available for all kind of organisms from mammals to E. coli. Glossary of Gateway cloning.... A Genetic Switch: Phage (Lambda) and Higher Organisms (Cambridge, MA: Cell Press). Additional Resources...

  18. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...definitions vary in the literature, but generally organisms with multi-drug resistance are resistant to three...another bacterium died for instance). These three mechanisms of acquired drug resistance are referred to as...is an intense amount of competition between microorganisms for available resources. This competition drives... drives microbes to develop survival mechanisms. Some microbes even make their own antibiotic compounds...

  19. CRISPR 101: Homology Directed Repair

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    Blog Post
    ...the template DNA. There are several unique HDR mechanisms, but all templated-repair pathways have these...process Homology directed repair pathways HDR mechanisms can be divided into two main camps: non-conservative...Shrivastav M, et al Cell Research (2008). PubMed. Mechanisms of DNA double-strand break repair and their potential...). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development 141; (2014). PubMed...

  20. Typing CRISPR Systems

    Type
    Blog Post
    ...time, scientists discovered many related immune mechanisms that could target foreign DNA with unique properties...share sequence similarity but are grouped due to mechanisms of action and other analyses. This also means... These subtypes have similar compositions and mechanisms, with the biggest differences being the components...2024). Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing applications...
Showing: 41 - 60 of 253 results