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We narrowed to 174 results for: tub

Showing: 41 - 60 of 174 results

  1. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...many different things do you simply refer to as a “tube”? We took steps towards solving this problem by...Prescribing an official name to all of our plates, tubes, and racks meant we had fewer errors in our LIMS...

  2. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...synthetic biologists to perform large scale, one-tube cloning reactions. In this blog, we’ll cover the...assemble multiple DNA fragments together in a one-tube reaction. Even though most MoClo reactions use Golden...

  4. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ... to use multiple techniques for a particularly stubborn prep. Check the bacterial strain Increase the...desired density. The volume of your flask or culture tube should be at least four times greater than your ...

  5. MXS Chaining

    Type
    Blog Post
    ...β-Actin Actin 4 Citrine 516nm/ 529nm human α-Tubulin Tubulin Repeatable, directional cloning   The ...

  6. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ..., mastered the art of finding how-to videos on YouTube long before he learned (somewhat successfully) ...                Two of the platforms we use at Addgene: YouTube, for longer educational videos, and Twitter, for...

  7. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Antibodies may even aggregate just from dropping the tube they are contained in (Randolph et. al., 2018). ...aliquoting and storage process to prevent dropped tubes. Now that your antibody is produced, harvested, ...

  8. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...dilutions. Aliquot all of your reagents into single-use tubes and grab a fresh one for each set up.  Wipe down... al. "Detection of circulating Mycobacterium tuberculosis-specific DNA by droplet digital PCR for vaccine...

  9. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...templates. All PCRs are run as an 18 cycle, 25 μl single-tube reaction. If you are synthesizing a new DNA fragment...Watson, and Ingo H. Greger. "IVA cloning: a single-tube universal cloning system exploiting bacterial in...

  10. Viral Production at Addgene

    Type
    Blog Post
    ...matching system that follows the sample from the DNA tube all the way to the cleared viral preparation pool...ensuring the identity of each sample from the DNA tube all the way to the viral preparation. After production...

  12. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...components into plants. Nanoparticles (carbon nanotubes, Kwak et al., 2019), DNA nanostructures (Zhang... using chitosan-complexed single-walled carbon nanotube carriers. Nat Nanotechnol 14:447–455 . https:/...

  13. All Antibodies

    Type
    Collection
    ...Markers : Antibodies targeting popular markers like tubulin or epitope tags like Myc, GFP, and more. Neuroscience...

  14. Adenovirus Plasmids

    Type
    Collection
    ... 5 (HAdV5) genome that can be assembled in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder...

  15. AAV Molecular Tools

    Type
    Collection
    ...Cre-dependent Cre-dependent expression of human 2N4R microtubule-associated protein tau with P301L mutation 1 ...

  16. mTOR Pathway

    Type
    Collection
    ...Ribosomal protein S6 kinase B1 TSC1/2 TSC1 TSC2 Tuberous sclerosis VHL Von Hippel-Lindau tumor suppressor...

  17. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...buffer called sheath fluid and transported through tubes or capillaries to a laser. As the cells move through...positive for a desired parameter is collected in a tube or plate, while a droplet containing a negative ...

  18. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...with all of the plasmids in the library in a single tube. The number of unique plasmids in a pooled library... submitted as 15 µL of DNA in a 1.5 mL microfuge tube at a concentration of 0.1–1 µg/µL (or bacterial ...

  19. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...plastic in spin columns and buffer bottles Waste tubes are made of 100% post-consumer recycled plastic ...buffers and columns sold separately Some, waste tubes sold separately No Other Minimized amount...

  20. p53 Pathway

    Type
    Collection
    ...3 Siah Siah E3 ubiquitin protein ligase 1 TSC2 Tuberous sclerosis 2 TSP1 Thrombospondin 1 Return to top...
Showing: 41 - 60 of 174 results