We narrowed to 1,076 results for: TOR;
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TypeBlog Post... in science brings stronger networking across laboratory and disciplinary boundaries and can allow for...
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A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...excitation wavelength, available emission filters, and detector sensitivity) and the biological system (temperature... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog Post...secondary antibodies are commonly used in research laboratories to detect the different subclasses, but there... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...these strains should not be used for long term storage. Additional Resources Review of restriction modification... -
How to Make Friends and Meet People at a Scientific Conference
TypeBlog Post...You never know when you will meet a potential collaborator Some of these people you meet will review your... -
Antibodies 101: Fab Fragments
TypeBlog Post...secondary antibodies you already have in your refrigerator will not recognize a Fab fragment. In order ... -
To Each HIS Own
TypeBlog Post...affinity purification of human insulin-like growth factor I from culture medium of E.coli. Bio/Technology... -
Transferable Skills Guide: Problem Solving
TypeBlog Post... you can start by paying attention to how your mentors reach solutions and ask them to explain their logic... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...Fishman JB, Berg EA (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https... -
Plasmids 101: Cre-lox
TypeBlog Post...independently of any other accessory proteins or co-factors, thus allowing for broad applications in a variety... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog Post...applied antibodies and need to dry the membrane for storage, but plan to reprobe it later, strip it thoroughly... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...specific, individual proteins under endogenous regulatory control. But even with the advent of CRISPR/Cas9... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog Post...quickly. Just make sure to incorporate the dilution factor in your calculation from OD to protein concentration... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post...high-quality antibodies for IHC. Working with collaborators and support from the Brain Research Through ... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...Mol Syst Biol. 2014 Galit Lahav EYFP Gateway vectors with EYFP split at 175 Arabidopsis homolog of the... -
Mouse Modeling, Part 2: Breeding and Crossing Mice
TypeBlog Post...the appropriate primers to detect your targeting vector or gene of interest. 2. Breed your parental mice... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...impacts in the way we study and treat complex or multifactorial diseases, enabling faster diagnosis and treatment... -
Plasmids 101: Degron Tags
TypeBlog Post...usual. In the presence of a specific protease inhibitor, asunaprevir, the viral protease can no longer... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post...) For CrispyCrunch, we wanted to consider both factors, as a bench scientist would looking at a genome... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post...RNA delivery does however rely on cloning into a vector or in vitro synthesis, which could be less desirable... -
Protocol - How to Perform a Diagnostic Digest
TypeProtocol...sites. Often, the size of the plasmid insert and vector backbone are known and thus this technique can ...Restriction Digest If you clone an insert into a vector by a single restriction enzyme, you will need to...which orientation the insert is ligated into the vector backbone and therefore the only thing left to do... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post...acoustic reporter genes for real-time in vivo monitoring of tumors and tumor-homing bacteria. Nat Biotechnol... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post...given therapeutic approach brings to patients, doctors, and insurance providers, and is crucial to how... -
Career Insights: Technical Support Specialist
TypeBlog Post... and exciting to listen to the many individual stories and get (sometimes refreshing) insights into how... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post...to RNA deaminase ADAR2 to create a specific RNA editor. Cas13 enzymes are advantageous because they do... -
Lab to Office Culture Shock
TypeBlog Post...industry, nonprofit organization, law office, journal editor, etc.--so everyone’s experience will be a little... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog Post.... The pDonor plasmid pRE57-Ter with multiple terminators can serve to interrupt genes (or gene clusters... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post...clone the PCR products into a pBluescript cloning vector and sequence the resulting plasmid. It can take... -
Molecular Biology Protocol - Restriction Digest of Plasmid DNA
TypeProtocol...cut the vector, you will need to use a phosphatase to prevent re-circularization of the vector if you ...having difficulty finding an enzyme that cuts your vector's multiple cloning site (MCS), but does not cut ... -
Immunocytochemistry
TypeProtocol...water Microscope slide Anti-fade mounting medium Laboratory wipes 15 mL conical tubes 50 mL conical tubes...paraformaldehyde and follow your institution's laboratory safety guidelines for disposing of waste in the...remove the coverslip. Blot the coverslip with a laboratory wipe to remove excess liquid. Add 1 drop of anti-fade...