We narrowed to 988 results for: igh

...editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR, Addgene gave to me: the PEDAR strategy...

...PES. Using a PES filter will maximize titer . DMEM high glucose, Corning 10-013-CV DMEM, low glucose (1 ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5...dissolved. Pro-Tip This step is challenging due to the high viscosity of PEG. Stirring under medium heat will...precipitation. Precipitation of the viruses can proceed overnight at 4 °C if needed. Transfer the entire sample ... prep . The clarified supernatant can be kept overnight at 4 °C before proceeding with the purification...

...Goedhart  Created in collaboration with Addgene writers High Complexity Golden Gate Assembly with NEB's DADExperts...

...annual #Deckthelab contest. The bar was set very high from last year’s impressive entries, but our community...

...with you.  Top 5 science comics:   1)   “Piled Higher and Deeper” by Jorge Cham   2)   “Boxplot” by Maki...

...Inoculating an Overnight Liquid Culture . After you have bacterial growth, add 500 μL of the overnight culture...bacteria onto an LB agar plate . Grow your bacteria overnight at the appropriate temperature. Growth conditions...page. The next day you will be able to start an overnight culture for plasmid DNA prep the following day...

...in 8,900 institutions across 111 countries. Just eight days into 2024, we've already shipped 2,078 items...

...journey. And if you have additional resources that might be helpful, please reach out to me by email.  About...

...topic page Read Joachim's Series on Choosing the B(right)est Fluorescent Protein Plasmids 101: Visualizing...

...organized by graduate student researchers. That’s right, #ActualLivingScientists, eager to communicate their...

...plasmids at Addgene, along with other tools like high resolution Ras sensors and crosslinked peptides ...

...and career goals, or to answer any questions you might have about Addgene. The page is divided into three...

...the Expression and Insert Species filters on the right-hand side of the search page to narrow your keyword...

... University with the backing of a prestigious Fulbright Fellowship. Dr. Kwapata now works as a lecturer...

...container Inverted fluorescence microscope Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5...each well of the 6-well dish. Incubate the cells overnight. If using freshly collected virus, filter through...If you anticipate that your viral stock will be higher titer, consider additional dilutions. Dilution ...

...winner will be determined by popular vote. That’s right - you get to choose the new name! Additionally, ...

...of the contest is our traditional approach. You might decorate your lab in pipette tip snowflakes, hang...

...fluorescent protein family that give off color in ambient light. Expressing these chromoproteins in E. coli would...

...plasmids at specific molar ratios. To get these ratios right, you need to do a few calculations. This calculator...

... about Crystal’s exciting career path, to gain insight into industry research, and to get advice on how...

...shape Addgene's future. Listen to this episode for insights into the process of getting an MD PhD, the inside...

...back on some fascinating Addgene history that you might not have known about. Here's the schedule for the...

...finally warming up in Boston and that means summer is right around the corner. Here at Addgene, we’re always...

...protocol may need to be adjusted to accommodate slight differences between products. Addgene does not ...mg/mL DAPI solution to 100 µL PBS. Protect from light. Prepare a 300 nM DAPI working solution by diluting...DAPI stock solution into 5 mL PBS. Protect from light. Procedure Section 1: Seeding cells Place a sterile...Pro-Tip The plate can be wrapped in foil to block light. Remove the secondary antibody and dispose of it...

...interneurons. Take a look to see if these new preps might enhance your research!   Table 1: New ready-to-use...

...plasmids shipped. Reaching one million plasmids shared might have you wondering how many plasmids leave Addgene...

... school for microbiology, and I ended up going straight to law school. This was in the early 80s, and ...

...open to industry scientists. This collection is highly diverse in terms of target organisms (from bacteria...

...for detecting genome edits, to discover why you might want to use Reddit to discuss your science and more...

...the labs they’re granted to, as well as shine a light on some of the great minds working in developing...