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Description of the components of the 4 most common viruses - AAV, adenovirus, lentivirus, and gamma-retrovirus.

A review of the cellular functions and consequences of dsRNA and RNA:DNA hybrids.

Get the basics on monoclonal antibodies including how monoclonal antibodies are made and why use monoclonal antibodies for your experiment.

Learn about new plasmids at Addgene including AAV split base editors, Ace2-mScarlet voltage indicator, CyanoGate MoClo cloning toolkit, and CreLite.

Learn about some newly developed Cas proteins and how to choose a Cas protein based on site accessibility, specificity, and sensitivity.

Learn about the different ways you can introduce DNA or RNA into cells using methods such as transformation, transduction, conjugation, and transfection.

FlipGFP is a genetically encoded caspase reporter that contains a flipped beta strand of GFP. There is no fluorescence unless the caspase cuts the cleavage site between the strands.

Targeted mutagenesis helps molecular biologists make changes to a DNA sequence at a precise location. Read on to learn more about EvolvR, a CRISPR-Cas9 based targeted mutagenesis method from the Dueber Lab.

Addgenie Eric Perkins curates Addgene resources you can use in your chromatin research.

Learn how dry ice shipment can affect pH and discover precautions you can take to protect your virus samples.

Learn how you can test your fluorescent protein's propensity to aggregate in your experiments.

Learn how you can use the Broad Target Accelerator Plasmid Collections from to help enable precision cancer medicine research.

Check out how you can use the top lentivirus and AAV from 2016 in your own research.

Learn how DNA methylation may affect the activity of certain restriction endonucleases.

Find tips on how to perform both large and small scale plasmid verification and sequencing.

Learn about how ClonTracer, a lentiviral library for lineage tracing, was used to test if cancer cells have resistance to drugs before or after treatment.

Learn more about non-homologous end joining (NHEJ) and how it relates to genome engineering technologies such as CRISPR/Cas9.

Overview of Cre-lox recombination and how it is used to make DNA modifications in transgenic animals, embryonic stem cells, and tissue-specific cell types.

Learn about click biology and how you can use it to advance your experiments and explore biological phenomenon.

Twenty years have gone by fast! Join us to share some highlights of Addgene and our community as we close out our 20th anniversary celebration.