We narrowed to 768 results for: Dos

1. Why and how to start studying chromatin (with Addgene's help)

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   Blog Post

   ... posts are not comprehensive, so just because you do not see a name or gene on this list, that does not...

2. CasPEDIA: A Functional Classification of Cas Enzymes

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   Blog Post

   ...resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: ...

3. Viral Vectors 101: Viral Vector Elements

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   Blog Post

   ...and are slightly different in sequence. Both LTRs do share common repeat regions in addition to their ...virion assembly and particle release from late endosomes when AAV is trafficked. Rep and cap mediate AAV...

4. Visualizing Protein Turnover In Situ

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   Blog Post

   ...blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize protein turnover within a neuron? For...

5. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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   Blog Post

   ... mitigating the pH effects of dry ice storage. To do this, we stored samples on dry ice for 72 hours and...

6. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   Blog Post

   ...with the microscope that you will be using. If you do a head-to-head comparison right, it will be valuable...

7. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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   Blog Post

   ...Central PMCID: PMC5120861. While there is much work to do, these early pilot studies have demonstrated technical...

8. Top Requested Lentivirus and AAV of 2016

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   Blog Post

   ...lentiviral inventory is based on CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9...

9. Addgene's Tips for Plasmid Quality Control

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   Blog Post

   ...sequences to visually check for homology and mismatches. Do you have your own best sequencing practices or maybe...

10. New Tool for Lineage Tracing: The ClonTracer Library

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    Blog Post

    ...and Validation Group and says that she decided to do a post-doc at Novartis because she wanted first-hand...

11. CRISPR 101: Non-Homologous End Joining

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    Blog Post

    ... ends better substrates for ligation. Although we do not describe these steps here, the processing of ...

12. Plasmids 101: Cre-lox

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    Blog Post

    ...Have you used the Cre-lox system in your research? Do you have any tips or tricks, or clever cre-dependent...

13. Luciferase Plasmid Collection

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    Collection

    ... Plasmid Description Gene/Insert PI Return to Top Do you have suggestions for other plasmids that should...protein interactions , protein dimerization , endosomal escape of cell-penetrating peptides , and more...

14. Tetracycline Inducible Expression

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    Collection

    ...Science and Technology (Link opens in a new window) Do you have suggestions for other plasmids that should...

15. CRISPR 101: Cas9 vs. The Other Cas(s)

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    Blog Post

    ...resources on the Addgene Blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? The PAM requirement...

16. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type

    Blog Post

    ...H134R) both reach the brain, but individual neurons do not express as many copies of the ChRs as compared...

17. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    Blog Post

    ...colony of genetically engineered mice, what can you do? In this post, we’ll cover why and how to cross mice...

18. RaPID Detection of RNA-protein Interactions

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    Blog Post

    ...RaPID can be used to study RNA-protein interactions. Do you have a question that RaPID could help answer?...

19. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    Blog Post

    ...References: Monticelli, M., Ferro, T., Jaeken, J., dos Reis Ferreira, V. & Videira, P. A. Immunological ...

20. Addgene: The First Twenty Years

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    Blog Post

    ...invaluable resource. I really appreciate the work you do to make sharing reagents and protocols so easy!” ...