We narrowed to 772 results for: Dos

1. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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   Blog Post

   ...spatio-temporal dynamics of apoptosis in living animals. To do this they modified a different self-assembling split...

2. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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   Blog Post

   ...we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment? The three years...

3. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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   Blog Post

   ...communication is not just for neurons. Non-neuronal cells do this too. It's just that for non-neural cells, ion-based...

4. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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   Blog Post

   ...interest into a bacterial cell or a host’s genome. To do this scientists commonly use phagemids, a DNA cloning...

5. Visualizing Protein Turnover In Situ

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   ...blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize protein turnover within a neuron? For...

6. Top Requested Lentivirus and AAV of 2016

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   Blog Post

   ...lentiviral inventory is based on CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9...

7. A Quick Guide to a Career in Software Product Management

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   Blog Post

   ...go to grad school. But, I didn’t know what else to do. Fortunately, the head of software product management...

8. Summer SciComm: Preprints

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   Blog Post

   ...broader community, and preprints are a great way to do that. We are happy to receive deposits of plasmids...

9. CasPEDIA: A Functional Classification of Cas Enzymes

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   Blog Post

   ...resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: ...

10. Viral Vectors 101: Viral Vector Elements

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    Blog Post

    ...and are slightly different in sequence. Both LTRs do share common repeat regions in addition to their ...virion assembly and particle release from late endosomes when AAV is trafficked. Rep and cap mediate AAV...

11. All in a Twist: dsRNA

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    Blog Post

    ...produced dsRNA rarely reach this threshold and thus do not typically activate an inflammatory response. ...

12. Antibodies 101: Polyclonal Antibodies

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    Blog Post

    ...strength of staining and background noise. If you do use different lots of your polyclonal antibody, you...

13. Addgene's Tips for Plasmid Quality Control

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    Blog Post

    ...sequences to visually check for homology and mismatches. Do you have your own best sequencing practices or maybe...

14. New Tool for Lineage Tracing: The ClonTracer Library

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    Blog Post

    ...and Validation Group and says that she decided to do a post-doc at Novartis because she wanted first-hand...

15. CRISPR 101: Non-Homologous End Joining

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    Blog Post

    ... ends better substrates for ligation. Although we do not describe these steps here, the processing of ...

16. Plasmids 101: Cre-lox

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    Blog Post

    ...Have you used the Cre-lox system in your research? Do you have any tips or tricks, or clever cre-dependent...

17. RaPID Detection of RNA-protein Interactions

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    Blog Post

    ...RaPID can be used to study RNA-protein interactions. Do you have a question that RaPID could help answer?...

18. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    Blog Post

    ...References: Monticelli, M., Ferro, T., Jaeken, J., dos Reis Ferreira, V. & Videira, P. A. Immunological ...

19. Human Germline Editing Using CRISPR

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    Blog Post

    ...diagnosis (PGD) screens embryos to find those that do not carry a given dominant disease-causing gene, ...

20. Multiplex Genome Editing with CRISPR-Cpf1

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    Blog Post

    ...: A New Tool for CRISPR Genome Editing Which Cas9 Do I Choose for My CRISPR Experiment A Match Made in...