We narrowed to 682 results for: RON

1. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   ...abilities to turn genes on and off,” he said. “Some are strong and some more moderate; there is an allelic series...

2. How to Make Friends and Meet People at a Scientific Conference

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   ... At A Scientific Conference Dinner - by Julio Peironcely on the Thesis Whisperer "Not Networking - Building...

3. Plasmids 101: Yeast Vectors

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   ...are considered “high copy”. A fragment from the 2 micron circle (a natural yeast plasmid) allows for 50+...

4. CRISPRainbow and Genome Visualization

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   ...successfully labeled protein-coding genes with both intronic and exonic gRNAs. In fact, the method is specific...

5. Troubleshooting Your Plasmid Cloning Experiment

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   ... the correct plasmid. There’s a lot that can go wrong! With all the steps in the cloning process, there...

6. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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   ...practices for reproducibility: data management plans, electronic lab notebooks, wet lab protocols, reagent sharing...

7. Click Biology: How to Click and Play to Explore Biology

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   ...Let Dye: Self-Labeling Protein Tags Plasmids 101: Degron Tags Additional Resources on addgene.org New ...

8. Summer SciComm: Preprints

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   ...quality. But even journal-based peer review can be prone to biases or errors, with a lack of transparency...

9. Antibodies 101: Affinity Reagents

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   ...10kD synthetic binding proteins which use the Fibronectin type III domain as their scaffolding instead ...

10. The time and cost required to make a plasmid

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    ... resources, and, on the rare occasion things go wrong, replace them for free. Plasmids ship out 2-3 days...

11. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    ...many FRET-opsin GEVIs. With an increase in the neuron’s membrane potential, Ace absorbance increases and...

12. Celebrating 15 Years of Scientific Sharing

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    ...the hands of our finance team, now 3.5 Addgenies strong. The Addgene Board of Directors is mature and...

13. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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    ...segment of the government, so it was a really good environment to be looking for a job.” Thanks to Susan for...

14. Addgene's Tips for Plasmid Quality Control

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    ...repetitive regions (such as LTRs or ITRs), hairpins, or strong terminators. Detailed instructions on other factors...

15. Plasmids 101: Golden Gate Cloning

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    ...multimers using class-IIS restriction enzymes. Lee JH, Skowron PM, Rutkowska SM, Hong SS, Kim SC. Genet Anal.1996...

16. Science communication: 9 Strategies to Get Your Foot in the Door

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    ...organisation like Building with Biology. Local environmental groups, charities and NGOs are often looking...

17. Plasmids for Endogenous Gene Tagging in Human Cells

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    ...tags (e.g. FLAG and STREP) and auxin-inducible degrons (AID). Further, the Yamamoto PITCh system plasmids...

18. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ... For plasmids with highly repetitive sequences (prone to recombination in bacteria) such as lentiviral...

19. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    ...intracellular injections into the cytoplasm or pronucleus of the zygote which is both technically challenging...

20. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    ...control mice will have been raised in the same environment and will harbor similar epigenetic modifications...