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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 704 results for: des.1

Showing: 581 - 600 of 704 results

  1. Improving Transduction Efficiency with Exosome AAVs

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    Blog Post
    ...vector-exosomes), exosome AAVs, or exo-AAVs (Figure 1). The researchers discovered that these particles ...producer cells and purification protocols often describe a process that begins with cell lysis to release...

  3. 3 Challenges in Plant Synthetic Biology

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    Blog Post
    ...challenges involved in plant synthetic biology. Challenge #1: Public perception of synthetic biology One of the...have been a model organism for plant research for decades, and the biochemical pathways involved in central...

  4. Viral Vectors 101: Viral Vector Elements

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    Blog Post
    ...guide outlines these components.     Fig. 1 - Viral vector components of retrovirus  Adeno-associated...promoter should flank this component as well. On both sides of this promoter-gene combo are two long terminal...Laboratory Press; 1997. Principles of Retroviral Vector Design. Available from: https://www.ncbi.nlm.nih.gov/books...

  5. GCE4All: Making Genetic Code Expansion Accessible

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    Blog Post
    ...non-canonical amino acid at the site of interest (figure 1). While the codon used is often one of the three stop...through a seminal paper from Peter Schultz’s lab describing a technique that allowed for intentional incorporation...reagents accessible to your lab. The solution GCE provides almost endless possibilities for researchers, ...

  6. Antibodies 101: Isotypes

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    Blog Post
    ...region: IgM, IgD, IgG, IgE, and IgA.    Figure 1: Diagram of an antibody labeled with Fc, Fab, heavy...molecules are recruited by the antibody to help destroy and remove a pathogen. Different isotypes also ...well as the genes that encode those proteins are designated by the lower-case Greek letters μ, δ, γ, ε, and...

  7. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...advantage this cycle, consider the advice below:  1. Eligibility check Scrutinize every opportunity prior... such as citizenship status, academic year, and grades that serve as automated filters of consideration...and cover letter revised by your network, which includes your friends, family members, and professional...

  8. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    Blog Post
    ...give these stabilized promoters a try. References 1. Segall-Shapiro, Thomas H., Eduardo D. Sontag, and...of copy number (Segall-Shapiro et al., 2018). Designing stabilized promoters To keep gene expression stable...ideally be optimized to promote generation of the desired product while avoiding the accumulation of toxic...

  9. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    Blog Post
    ...native peptide bond at the split site. Figure 1: The base editor is split into two plasmids and co-transfected...showing the potential for broad use. This kit is designed for use with acceptor vectors from the MoClo Toolkit...tracing Article contributed by Erin Sanders CreLite, designed by the Eisenhoffer lab, is a new tool that increases...

  10. Plasmids 101: Cre-lox

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    Blog Post
    ...techniques?  Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination. I. ...using a resistance marker; however, it is often desirable to remove the marker after the initial selection... Combining this with some of the loxP methods described above, a genetic modification can be restricted...

  11. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post
    ...internalization of two different receptors- angiotensin II type 1 receptor and the β2 adrenoceptor. GPCRs in chemogenetics...new receptors were termed Designer Receptor Exclusively Activated by Designer Drugs (DREADDs) (Roth, 2016...What do smell, taste and sight have in common, besides being one of the five senses? GPCRs or G-protein-coupled...photons or odorants, to elicit downstream signaling cascades.  So let’s break down the name "G-protein coupled...then induce or inhibit intracellular signaling cascades as a response to the extracellular stimuli. Ligand...

  12. What's New in CRISPR - November 2021

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    Blog Post
    ... Science Find the SEND plasmids    Figure 1: SEND combines an endogenous Gag homolog, cargo RNA...different labs have recently published work that provides a solution to this problem. A miniature CRISPR-Cas...miniature CRISPR-Cas12f nuclease Quanjiang Ji’s lab describes an effective miniature Cas system that utilizes...Nature Biotechnology paper showcases an improved design of the guide RNA that caps the 3’ end with a structured...that mismatch repair (MMR) interfered with the desired resolution of prime editing intermediates. Building...sequence. As with other prime editing tools, the desired edits are templated by the 3′ extension of the ...

  13. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post
    ...incorporated at the termini of the crRNA molecule (usually 1-3 of the first 3 bps are modified) for optimal stability...to simply swap out several of the ribonucleotides for deoxynucleotides. A partially DNA gRNA is surprisingly...use in applications like siRNA and antisense oligonucleotides. Some of these synthetic modifications, specifically...photoactivatable and photocleavable gRNAs. Photocleavable guides are generated by introduction of a single photocleavable...Additional resources on the Addgene Blog  How to design your CRISPR gRNA CRISPR 101: Ribonucleoprotein ...

  14. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ... an entirely new affinity reagent.    Fig. 1: A comparison of an antibody (left) to several alternative... as the OG (as the kids say) affinity reagents deserved to be celebrated, there are many other amazing...its thorns and every affinity reagent has its downsides. These reduced-in-size options often have reduced...°C and can refold correctly after denaturing.  Designed ankyrin repeat proteins, or DARPins, are genetically...a wide variety of targets, including proteins, peptides, amino acids, drugs, metal ions, and cells. They...

  15. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    Blog Post
    ...see how you use them in your research!  References 1.Tang, Jonathan C.Y., et al. “A nanobody-based system...GFP-labeled cells. Once Tang and Cepko found a description of GFP-binding nanobodies, the project really...stable inside cells. Nanobodies binding GFP were designed in 2009. Subsequent work showed that nanobodies...green-red Cre-lox systems. T-DDOGs can also be designed with other DNA-binding domains, including the ...

  16. Viral Vectors 101: An Introduction to AAV

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    Blog Post
    ...many of which are available in rAAVs!   Figure 1: Diagram of AAV components      Cargo capacity...serotypes The names of AAVs contain two numerical designations, separated by a slash, that indicate the ITR...interested in expressing multiple small genes, you can design single rAAV vectors with multiple genes packaged... an option (McCarty et al., 2001). This AAV is designed so that the single stranded DNA folds back on ...

  17. Viral Vectors 101: Types of viruses

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    Blog Post
    ...efficiently with adenovirus and AAV.   Table 1: Types of viruses and their features Choosing ...about a specific virus? We have you covered with guides on lentivirus, retrovirus, adenovirus, and AAV....

  18. Antibodies 101: Secondary Antibodies

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    Blog Post
    ...with each protein of interest.    Figure 1: The direct approach (left) versus the indirect approach...affinity, many indirect antibody protocols are designed to take place over multiple days. Increased run...
Showing: 581 - 600 of 704 results