We narrowed to 718 results for: des.1

...through the use of nanoparticles. [6].    References 1. Zhang B. 2021. “CRISPR/Cas gene therapy.” Journal...ability to influence editing efficiency. In 2022, despite the increasing effectiveness of CRISPR/Cas9, scientists...

...pitfalls? Here's what you need to know.   Figure 1: Management can seem a mystery at first! Image used...focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with actionable steps...

...challenges involved in plant synthetic biology. Challenge #1: Public perception of synthetic biology One of the...have been a model organism for plant research for decades, and the biochemical pathways involved in central...

...guide outlines these components.     Fig. 1 - Viral vector components of retrovirus  Adeno-associated...promoter should flank this component as well. On both sides of this promoter-gene combo are two long terminal...Laboratory Press; 1997. Principles of Retroviral Vector Design. Available from: https://www.ncbi.nlm.nih.gov/books...

...non-canonical amino acid at the site of interest (figure 1). While the codon used is often one of the three stop...through a seminal paper from Peter Schultz’s lab describing a technique that allowed for intentional incorporation...reagents accessible to your lab. The solution GCE provides almost endless possibilities for researchers, ...

...region: IgM, IgD, IgG, IgE, and IgA.    Figure 1: Diagram of an antibody labeled with Fc, Fab, heavy...molecules are recruited by the antibody to help destroy and remove a pathogen. Different isotypes also ...well as the genes that encode those proteins are designated by the lower-case Greek letters μ, δ, γ, ε, and...

...advantage this cycle, consider the advice below:  1. Eligibility check Scrutinize every opportunity prior... such as citizenship status, academic year, and grades that serve as automated filters of consideration...and cover letter revised by your network, which includes your friends, family members, and professional...

...give these stabilized promoters a try. References 1. Segall-Shapiro, Thomas H., Eduardo D. Sontag, and...of copy number (Segall-Shapiro et al., 2018). Designing stabilized promoters To keep gene expression stable...ideally be optimized to promote generation of the desired product while avoiding the accumulation of toxic...

...native peptide bond at the split site. Figure 1: The base editor is split into two plasmids and co-transfected...showing the potential for broad use. This kit is designed for use with acceptor vectors from the MoClo Toolkit...tracing Article contributed by Erin Sanders CreLite, designed by the Eisenhoffer lab, is a new tool that increases...

...techniques?  Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination. I. ...using a resistance marker; however, it is often desirable to remove the marker after the initial selection... Combining this with some of the loxP methods described above, a genetic modification can be restricted...

...internalization of two different receptors- angiotensin II type 1 receptor and the β2 adrenoceptor. GPCRs in chemogenetics...new receptors were termed Designer Receptor Exclusively Activated by Designer Drugs (DREADDs) (Roth, 2016...What do smell, taste and sight have in common, besides being one of the five senses? GPCRs or G-protein-coupled...photons or odorants, to elicit downstream signaling cascades.  So let’s break down the name "G-protein coupled...then induce or inhibit intracellular signaling cascades as a response to the extracellular stimuli. Ligand...

... Science Find the SEND plasmids    Figure 1: SEND combines an endogenous Gag homolog, cargo RNA...different labs have recently published work that provides a solution to this problem. A miniature CRISPR-Cas...miniature CRISPR-Cas12f nuclease Quanjiang Ji’s lab describes an effective miniature Cas system that utilizes...Nature Biotechnology paper showcases an improved design of the guide RNA that caps the 3’ end with a structured...that mismatch repair (MMR) interfered with the desired resolution of prime editing intermediates. Building...sequence. As with other prime editing tools, the desired edits are templated by the 3′ extension of the ...

... an entirely new affinity reagent.    Fig. 1: A comparison of an antibody (left) to several alternative... as the OG (as the kids say) affinity reagents deserved to be celebrated, there are many other amazing...its thorns and every affinity reagent has its downsides. These reduced-in-size options often have reduced...°C and can refold correctly after denaturing.  Designed ankyrin repeat proteins, or DARPins, are genetically...a wide variety of targets, including proteins, peptides, amino acids, drugs, metal ions, and cells. They...

...many of which are available in rAAVs!   Figure 1: Diagram of AAV components      Cargo capacity...serotypes The names of AAVs contain two numerical designations, separated by a slash, that indicate the ITR...interested in expressing multiple small genes, you can design single rAAV vectors with multiple genes packaged... an option (McCarty et al., 2001). This AAV is designed so that the single stranded DNA folds back on ...

...see how you use them in your research!  References 1.Tang, Jonathan C.Y., et al. “A nanobody-based system...GFP-labeled cells. Once Tang and Cepko found a description of GFP-binding nanobodies, the project really...stable inside cells. Nanobodies binding GFP were designed in 2009. Subsequent work showed that nanobodies...green-red Cre-lox systems. T-DDOGs can also be designed with other DNA-binding domains, including the ...

...efficiently with adenovirus and AAV.   Table 1: Types of viruses and their features Choosing ...about a specific virus? We have you covered with guides on lentivirus, retrovirus, adenovirus, and AAV....

...with each protein of interest.    Figure 1: The direct approach (left) versus the indirect approach...affinity, many indirect antibody protocols are designed to take place over multiple days. Increased run...

... trafficking of these biosensors.   Figure 1: Schematic of iGluSnFR3 (left). GluSnFR3 in pMinDisplay...sulfurtransferase (MPST) which catalyzes the desulfuration of 3-mercaptopyruvate and generates an enzyme-bound...transferred to thiol-containing acceptors. These hydropersulfides modulate protein activity and have important...

...manipulation, there are two main decisions to make: 1. Which modification? and 2. Which viral vector should...behavioral neuroscience, and address experimental design considerations used in all behavioral studies. ...ion channels. Similarly, chemogenetic tools are designed channels that are chemically activated. Both are...

... – a mechanical device that could be used to bend 1/8th inch steel wire into a backbone structure of a...BioMolecular Modeling and the CEO of 3-D Molecular Designs. Have you ever held your favorite protein in the...favorite protein in your hand? At 3D Molecular Designs, we create physical models of proteins using 3D...off a small, family-run business – 3D Molecular Designs – to commercialize some of the best instructional...reaction to this model on his blog. Or, for a description of how effective this model was in explaining... up close and personal, visit the 3D Molecular Designs web site. Or, if you are visiting a local classroom...check out our hands-on teaching tools that are designed to make the molecular world real for students....