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We narrowed to 955 results for: ARC;

Showing: 601 - 620 of 955 results

  1. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...would be directly reported by band intensity. Researchers use later steps in this chain (blue) to infer...controls (green). Created with BioRender.com. Researchers often rely on assumptions that their loading ...your particular setup. We recommend starting by searching the literature to see what is already known about...

  2. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ... generate full libraries of mutant proteins. Researchers from the Whitehead lab developed One pot saturation...saturation mutagenesis? How would you use it in your research? References 1. Natarajan, Chandrasekhar, et al...Central PMCID: PMC3159719. 6. Firnberg, Elad, and Marc Ostermeier. “PFunkel: Efficient, Expansive, User-Defined...

  3. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...application for basic and translational research.  Basic research applications of scFvs: The SunTag system...Plasmid Collection Find antibodies for neuroscience research from the Trimmer lab Check out Addgene's latest...

  4. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ... COVID-19 pandemic. The Pandemic Pivot Back in March 2020, just four months after I started with Addgene...completely out of reach for Addgene.  So, onto research! I needed to design a system that would allow ...  Quintin Marcelino...

  5. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...efficiency of homology directed repair, with researchers hypothesizing that they can efficiently integrate...bp sequence around the desired insertion site to search for potential gRNAs. As with other homology-directed... under a Creative Commons 4.0 license. The research groups responsible for Easi-CRISPR have also recently...

  6. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...CRISPR Plasmids for Your Research Find CRISPR Pooled Libraries for Your Research ... human stem cell genome editing. Nucleic Acids Research 41:9049–9061 . https://doi.org/10.1093/nar/gkt555...

  7. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...mouse genome. With these early technologies, a researcher would first introduce a mutation into a mouse...and rushed to test it. I still remember the day, March 16, 2014, that I got my first preliminary results...CRISPR-Cas9 is an acquired immune system in bacteria and archaea. As you know well if you’ve been following the ...

  8. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...couldn’t be luckier with my mentor, since she does research at UK in the same field that I do: prostate cancer...highlights included remarks by the Spanish Secretary of Research, Development, and Innovation, D. Carmen Vela, ...    Rosario Fernandez-Godino is a Postdoctoral Researcher at MEEI-Harvard Medical School, where she studies...

  9. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...stability, or other properties is an active area of research. If size is a concern for your application, SNAP-tag...ligand to use for your experiment. Conclusion Researchers and vendors continue to develop new ligands for...Proteins Inside Living Cells. Accounts of Chemical Research, 44(9), 784–792. https://doi.org/10.1021/ar200099f...

  10. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...high levels of spurious expression.  As such, researchers developed new tools called FLEx/DIO vectors where...expression levels over several weeks allows the researcher to determine when the optimal time to conduct...merely a new lot, be thoroughly tested in the researcher's system before beginning large scale experiments...

  11. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...during shipping. Of the kits reviewed here, NEB’s Monarch®, QIAGEN’s QIAwave, and Milllipore Sigma’s GenElute...in plasmid purification kits Kit Series NEB Monarch QIAGEN QIAwave Millipore Sigma GenElute Reduced...recycled materials Sustainable forestry certification Starch-based compostable bags for kit components Recyclable...

  12. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...Cpf1.   Figure 1: Multiplexing allows researchers to express multiple gRNA from a single construct...from a single lentiviral vector.” Nucleic Acids Research 42(19) (2014): e147. PubMed PMID: 25122746. PubMed... Our Guide Pages Find CRISPR Plasmids for Your Research Browse Our CRISPR Multiplexing Resources ...

  14. 2022 Depositor Week

    Type
    Blog Post
    ...say thank you to all the many scientists and researchers who deposit with us!  This year alone, we had...Depositors Hall of Fame  Thursday: 8 Great Depositor Research Stories   Friday: Specialized Depositor Shout-...

  15. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    ...looking to use antibody-based applications in their research.  Like all our eBooks, the Antibodies 101 eBook...For example, if you’re very new to antibodies as research tools, you may find “Chapter 1: What is an Antibody...

  16. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

    Type
    Blog Post
    ...evidence that laughter really can be good for you. A search on PubMed for the word “laughter” turns up a few...evidence. (What can I say, we are scientists). Researchers in Taiwan showed that a laughter program significantly...

  18. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    ...I spent about seven years working in research labs, and then pivoted to writing full-time in mid-2020...synthetic biology. The guide is a curated list of research papers, blogs, free courses, YouTube channels,...

  19. Twenty Years of Sharing Science!

    Type
    Blog Post
    [email protected]! Fun fact! Did you know you can search for plasmids from a publication by pasting the ...the paper's title into the Addgene website search bar?   We'll be celebrating all year long, so keep an ...

  20. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ...communication is required to promote public support for research and to keep useful discoveries coming. At Addgene...are all run and organized by graduate student researchers. That’s right, #ActualLivingScientists, eager...
Showing: 601 - 620 of 955 results