...be targeted with optical stimuli. The restricted light exposure to a specific area in combination with ...

... Since the signal level provided by SunTag is so high, cells may be imaged under lower illumination settings...

...productivity by providing world-wide access to curated, high-quality data and reagents Addgene makes it easy ...

...’s not our only job - we strive to also give you high-quality educational resources to help you do better...

...faculty and introducing the kits to clients who might initially be doubtful of online science lab classes...

...consider. Antibiotic selection Selection is straightforward in cases where the inserted DNA includes an...

...document may sound like something out of your nightmares, shared ownership of materials produced from ...

...troubleshooting tips to make plasmid usage as simple and straightforward as possible. If you have a question about a...

...pinpoint exactly when I realized I had made the right decision to choose Addgene for my co-op, but I know...

...Antibody dependent cellular cytotoxicity (ADCC) is a slightly more direct method than opsonization. In ADCC,...

...recommended names and synonyms will all get you to the right entry.) You can look up the cell lines you are currently...

...an opportune time or go all out with constitutive high level expression? Finally, you can add bells and...

...container Inverted fluorescence microscope Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5...each well of the 6-well dish. Incubate the cells overnight. If using freshly collected virus, filter through...If you anticipate that your viral stock will be higher titer, consider additional dilutions. Dilution ...

...fertilizer, seeds and a growth space with fluorescent lights. Other experiments require only seeds, petri dishes...

...biotin-avidin binding could provide a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions...

...elements Coding sequence features are relatively straightforward to define, but for control elements such as...

...serotypes are equal; while some serotypes are typically high yielding, for others, such as AAV2, low yields are...

... the same protein, their gene sequence may have slight or large variations depending on how closely related...

...of troubleshooting failed experiments. Pick the right strain While common E. coli strains like DH5-alpha...

...-enveloped virus (left) and an enveloped virus (right). Created using biorender.com   If you’re new to...

...amplify your insert DNA. It is important to use a high fidelity polymerase to minimize mutations. The fidelity...

...Then ask for their honest opinions -- this part might hurt, but if you pitch to enough people, you will...

... and human embryonic stem cells, they found very high recombination percentages (78-79% after two rounds...

...Watson-Crick base pairing, allowing for rational, straightforward design. Since the technique relies on base ...

...knowledge to impart. An undergraduate can mentor a high school student, a graduate student can mentor an...

...protocol may need to be adjusted to accommodate slight differences between products. Addgene does not ...mg/mL DAPI solution to 100 µL PBS. Protect from light. Prepare a 300 nM DAPI working solution by diluting...DAPI stock solution into 5 mL PBS. Protect from light. Procedure Section 1: Seeding cells Place a sterile...Pro-Tip The plate can be wrapped in foil to block light. Remove the secondary antibody and dispose of it...

...controller Hazardous waste container Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat inactivated FBS and 5...colonies for at least 2 of the dilutions. Pro-Tip The higher dilution wells tend to provide more accurate titers...dilutions, and single colonies are visible in the higher dilutions. Last reviewed on: August 31, 2023...

...and, depending upon who your challenger was, you might have to drastically alter your strategy. I realized...

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...back actual magic (which had been lost for an age) fight in the Napoleonic Wars and against something even...