...not a big issue for cells, but it may confound research results or even kill your cells if your gene of...right tissue-specific promoters as described by researchers at the NIH here.  Note: A. Max Juchheim contributed...Resources on Addgene.org Find Plasmids for Your Research     ...

... generate full libraries of mutant proteins. Researchers from the Whitehead lab developed One pot saturation...saturation mutagenesis? How would you use it in your research? References 1. Natarajan, Chandrasekhar, et al...Central PMCID: PMC3159719. 6. Firnberg, Elad, and Marc Ostermeier. “PFunkel: Efficient, Expansive, User-Defined...

...application for basic and translational research.  Basic research applications of scFvs: The SunTag system...Plasmid Collection Find antibodies for neuroscience research from the Trimmer lab Check out Addgene's latest...

... COVID-19 pandemic. The Pandemic Pivot Back in March 2020, just four months after I started with Addgene...completely out of reach for Addgene.  So, onto research! I needed to design a system that would allow ...  Quintin Marcelino...

...would be directly reported by band intensity. Researchers use later steps in this chain (blue) to infer...controls (green). Created with BioRender.com. Researchers often rely on assumptions that their loading ...your particular setup. We recommend starting by searching the literature to see what is already known about...

...mouse genome. With these early technologies, a researcher would first introduce a mutation into a mouse...and rushed to test it. I still remember the day, March 16, 2014, that I got my first preliminary results...CRISPR-Cas9 is an acquired immune system in bacteria and archaea. As you know well if you’ve been following the ...

...couldn’t be luckier with my mentor, since she does research at UK in the same field that I do: prostate cancer...highlights included remarks by the Spanish Secretary of Research, Development, and Innovation, D. Carmen Vela, ...    Rosario Fernandez-Godino is a Postdoctoral Researcher at MEEI-Harvard Medical School, where she studies...

...efficiency of homology directed repair, with researchers hypothesizing that they can efficiently integrate...bp sequence around the desired insertion site to search for potential gRNAs. As with other homology-directed... under a Creative Commons 4.0 license. The research groups responsible for Easi-CRISPR have also recently...

...CRISPR Plasmids for Your Research Find CRISPR Pooled Libraries for Your Research ... human stem cell genome editing. Nucleic Acids Research 41:9049–9061 . https://doi.org/10.1093/nar/gkt555...

...high levels of spurious expression.  As such, researchers developed new tools called FLEx/DIO vectors where...expression levels over several weeks allows the researcher to determine when the optimal time to conduct...merely a new lot, be thoroughly tested in the researcher's system before beginning large scale experiments...

...stability, or other properties is an active area of research. If size is a concern for your application, SNAP-tag...ligand to use for your experiment. Conclusion Researchers and vendors continue to develop new ligands for...Proteins Inside Living Cells. Accounts of Chemical Research, 44(9), 784–792. https://doi.org/10.1021/ar200099f...

...Cpf1.   Figure 1: Multiplexing allows researchers to express multiple gRNA from a single construct...from a single lentiviral vector.” Nucleic Acids Research 42(19) (2014): e147. PubMed PMID: 25122746. PubMed... Our Guide Pages Find CRISPR Plasmids for Your Research Browse Our CRISPR Multiplexing Resources ...

...during shipping. Of the kits reviewed here, NEB’s Monarch®, QIAGEN’s QIAwave, and Milllipore Sigma’s GenElute...in plasmid purification kits Kit Series NEB Monarch QIAGEN QIAwave Millipore Sigma GenElute Reduced...recycled materials Sustainable forestry certification Starch-based compostable bags for kit components Recyclable...

...chromosomal DNA. Although they can be found in archaea and eukaryotes, they play the most significant ...eukaryotic, as well as common promoters used in research check out our promoters reference page . Selectable...fragmented into small pieces, labeled with a short DNA barcode, and amplified. These DNA fragments are attached...information to aid in the reproducibility of scientific research. Resources Genetic Code The genetic code can be...newest breakthroughs in plasmid technologies and research, to overviews of molecular biology basics and ...

...interesting view into how rapid innovation affects the research community. I’ve likened it to the celebration ...currently at in the CRISPR software space. The CRISPR research community hasn't had a chance to develop a consensus...technology has been surrounded by a whirlwind of research activity and development since its inaugural year...

...transcription actually begins. Only eukaryotes and archaea, however, contain this TATA box. Most prokaryotes... proteins. Common Eukaryotic Promoters Used in Research Promoter Expression Description CMV Constitutive...controlled operon. Common Bacterial Promoters used in Research Promoter Expression Description T7 Constitutive...newest breakthroughs in plasmid technologies and research to overviews of molecular biology basics and plasmid...

...say thank you to all the many scientists and researchers who deposit with us!  This year alone, we had...Depositors Hall of Fame  Thursday: 8 Great Depositor Research Stories   Friday: Specialized Depositor Shout-...

...looking to use antibody-based applications in their research.  Like all our eBooks, the Antibodies 101 eBook...For example, if you’re very new to antibodies as research tools, you may find “Chapter 1: What is an Antibody...

...evidence that laughter really can be good for you. A search on PubMed for the word “laughter” turns up a few...evidence. (What can I say, we are scientists). Researchers in Taiwan showed that a laughter program significantly...

[email protected]! Fun fact! Did you know you can search for plasmids from a publication by pasting the ...the paper's title into the Addgene website search bar?   We'll be celebrating all year long, so keep an ...